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Reduction of proliferating non-neuronal cells in dried nerve segments partly impairs nerve regeneration

Widerberg, Annika LU and Dahlin, Lars LU (2000) In NeuroReport 11(12). p.2707-2707
Abstract
To determine whether drying of nerve grafts can affect axonal outgrowth and proliferation of non-neuronal cells, nerve segments dried for 0-60 min were used as nerve grafts to bridge a gap in transected rat sciatic nerves. Axonal outgrowth was measured by pinch reflex test and confirmed by immunocytochemical staining of neurofilaments. The proliferation of non-neuronal cells was measured by incorporation of BrdU in the dried nerve segments. Drying of the nerve segment for 60 min reduced the length of axonal outgrowth to 66 and 76% 3 and 6 days, respectively, after the grafting procedure. At that time point the number of proliferating cells was reduced by 51%. It is concluded that the number of proliferating non-neuronal cells is reduced in... (More)
To determine whether drying of nerve grafts can affect axonal outgrowth and proliferation of non-neuronal cells, nerve segments dried for 0-60 min were used as nerve grafts to bridge a gap in transected rat sciatic nerves. Axonal outgrowth was measured by pinch reflex test and confirmed by immunocytochemical staining of neurofilaments. The proliferation of non-neuronal cells was measured by incorporation of BrdU in the dried nerve segments. Drying of the nerve segment for 60 min reduced the length of axonal outgrowth to 66 and 76% 3 and 6 days, respectively, after the grafting procedure. At that time point the number of proliferating cells was reduced by 51%. It is concluded that the number of proliferating non-neuronal cells is reduced in dried nerve segments which only partly impairs axonal outgrowth. Factors other than Schwann cells are probably important for an optimal mileue for regeneration in nerve grafts. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
NeuroReport
volume
11
issue
12
pages
2707 - 2707
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:10976948
  • scopus:0034698976
ISSN
1473-558X
language
English
LU publication?
yes
id
be87604d-82d5-4c5a-8088-566e30ef6d19 (old id 1117725)
date added to LUP
2008-06-25 09:49:32
date last changed
2017-01-01 05:08:15
@article{be87604d-82d5-4c5a-8088-566e30ef6d19,
  abstract     = {To determine whether drying of nerve grafts can affect axonal outgrowth and proliferation of non-neuronal cells, nerve segments dried for 0-60 min were used as nerve grafts to bridge a gap in transected rat sciatic nerves. Axonal outgrowth was measured by pinch reflex test and confirmed by immunocytochemical staining of neurofilaments. The proliferation of non-neuronal cells was measured by incorporation of BrdU in the dried nerve segments. Drying of the nerve segment for 60 min reduced the length of axonal outgrowth to 66 and 76% 3 and 6 days, respectively, after the grafting procedure. At that time point the number of proliferating cells was reduced by 51%. It is concluded that the number of proliferating non-neuronal cells is reduced in dried nerve segments which only partly impairs axonal outgrowth. Factors other than Schwann cells are probably important for an optimal mileue for regeneration in nerve grafts.},
  author       = {Widerberg, Annika and Dahlin, Lars},
  issn         = {1473-558X},
  language     = {eng},
  number       = {12},
  pages        = {2707--2707},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {NeuroReport},
  title        = {Reduction of proliferating non-neuronal cells in dried nerve segments partly impairs nerve regeneration},
  volume       = {11},
  year         = {2000},
}