Activation of neutral sphingomyelinase participates in ethanol-induced apoptosis in Hep G2 cells
(2000) In Alcohol and Alcoholism 35(6). p.569-573- Abstract
- The mechanism underlying ethanol-induced apoptosis in liver cells is not clear. Sphingomyelin (SM) metabolism is a novel signal transduction pathway that has an impact on apoptosis in many cell types. We investigated whether the SM pathway is involved in ethanol-induced apoptosis in the liver. Hep G2 cells were treated with ethanol followed by assaying apoptosis, sphingomyelinase (SMase) activity, caspase-3 activity, and the changes of SM content in the cells. We found that ethanol dose-dependently increased apoptosis and the effect was accompanied by increases of caspase-3 activity and neutral SMase activity. At concentrations of 80 and 160 mM, ethanol significantly increased caspase-3 activity by 120% and neutral SMase activity by 24%.... (More)
- The mechanism underlying ethanol-induced apoptosis in liver cells is not clear. Sphingomyelin (SM) metabolism is a novel signal transduction pathway that has an impact on apoptosis in many cell types. We investigated whether the SM pathway is involved in ethanol-induced apoptosis in the liver. Hep G2 cells were treated with ethanol followed by assaying apoptosis, sphingomyelinase (SMase) activity, caspase-3 activity, and the changes of SM content in the cells. We found that ethanol dose-dependently increased apoptosis and the effect was accompanied by increases of caspase-3 activity and neutral SMase activity. At concentrations of 80 and 160 mM, ethanol significantly increased caspase-3 activity by 120% and neutral SMase activity by 24%. The activity of acid SMase was only slightly increased without statistical significance. C(2)-ceramide, the exogenous SM metabolite, mimicked the effects of ethanol on apoptosis and caspase-3 activation. When the SM content was determined 24 h after treatment with ethanol, its level was 15% lower than that of controls. The results indicate that metabolism of SM triggered by neutral SMase participates in ethanol-induced apoptosis in Hep G2 cells and activation of caspase-3 is involved in the apoptotic pathway. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1117909
- author
- Liu, J J ; Wang, J Y ; Hertervig, Erik LU ; Cheng, Yajun LU ; Nilsson, Åke LU and Duan, Rui-Dong LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Alcohol and Alcoholism
- volume
- 35
- issue
- 6
- pages
- 569 - 573
- publisher
- Oxford University Press
- external identifiers
-
- pmid:11093963
- scopus:0033662390
- ISSN
- 1464-3502
- language
- English
- LU publication?
- yes
- id
- 336608cb-9501-4b49-9e0f-90d7371cf192 (old id 1117909)
- alternative location
- http://alcalc.oxfordjournals.org/cgi/content/abstract/35/6/569
- date added to LUP
- 2016-04-01 12:21:00
- date last changed
- 2024-10-09 06:54:17
@article{336608cb-9501-4b49-9e0f-90d7371cf192, abstract = {{The mechanism underlying ethanol-induced apoptosis in liver cells is not clear. Sphingomyelin (SM) metabolism is a novel signal transduction pathway that has an impact on apoptosis in many cell types. We investigated whether the SM pathway is involved in ethanol-induced apoptosis in the liver. Hep G2 cells were treated with ethanol followed by assaying apoptosis, sphingomyelinase (SMase) activity, caspase-3 activity, and the changes of SM content in the cells. We found that ethanol dose-dependently increased apoptosis and the effect was accompanied by increases of caspase-3 activity and neutral SMase activity. At concentrations of 80 and 160 mM, ethanol significantly increased caspase-3 activity by 120% and neutral SMase activity by 24%. The activity of acid SMase was only slightly increased without statistical significance. C(2)-ceramide, the exogenous SM metabolite, mimicked the effects of ethanol on apoptosis and caspase-3 activation. When the SM content was determined 24 h after treatment with ethanol, its level was 15% lower than that of controls. The results indicate that metabolism of SM triggered by neutral SMase participates in ethanol-induced apoptosis in Hep G2 cells and activation of caspase-3 is involved in the apoptotic pathway.}}, author = {{Liu, J J and Wang, J Y and Hertervig, Erik and Cheng, Yajun and Nilsson, Åke and Duan, Rui-Dong}}, issn = {{1464-3502}}, language = {{eng}}, number = {{6}}, pages = {{569--573}}, publisher = {{Oxford University Press}}, series = {{Alcohol and Alcoholism}}, title = {{Activation of neutral sphingomyelinase participates in ethanol-induced apoptosis in Hep G2 cells}}, url = {{http://alcalc.oxfordjournals.org/cgi/content/abstract/35/6/569}}, volume = {{35}}, year = {{2000}}, }