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Activation of neutral sphingomyelinase participates in ethanol-induced apoptosis in Hep G2 cells

Liu, J J; Wang, J Y; Hertervig, Erik LU ; Cheng, Yajun LU ; Nilsson, Åke LU and Duan, Rui-Dong LU (2000) In Alcohol and Alcoholism 35(6). p.569-573
Abstract
The mechanism underlying ethanol-induced apoptosis in liver cells is not clear. Sphingomyelin (SM) metabolism is a novel signal transduction pathway that has an impact on apoptosis in many cell types. We investigated whether the SM pathway is involved in ethanol-induced apoptosis in the liver. Hep G2 cells were treated with ethanol followed by assaying apoptosis, sphingomyelinase (SMase) activity, caspase-3 activity, and the changes of SM content in the cells. We found that ethanol dose-dependently increased apoptosis and the effect was accompanied by increases of caspase-3 activity and neutral SMase activity. At concentrations of 80 and 160 mM, ethanol significantly increased caspase-3 activity by 120% and neutral SMase activity by 24%.... (More)
The mechanism underlying ethanol-induced apoptosis in liver cells is not clear. Sphingomyelin (SM) metabolism is a novel signal transduction pathway that has an impact on apoptosis in many cell types. We investigated whether the SM pathway is involved in ethanol-induced apoptosis in the liver. Hep G2 cells were treated with ethanol followed by assaying apoptosis, sphingomyelinase (SMase) activity, caspase-3 activity, and the changes of SM content in the cells. We found that ethanol dose-dependently increased apoptosis and the effect was accompanied by increases of caspase-3 activity and neutral SMase activity. At concentrations of 80 and 160 mM, ethanol significantly increased caspase-3 activity by 120% and neutral SMase activity by 24%. The activity of acid SMase was only slightly increased without statistical significance. C(2)-ceramide, the exogenous SM metabolite, mimicked the effects of ethanol on apoptosis and caspase-3 activation. When the SM content was determined 24 h after treatment with ethanol, its level was 15% lower than that of controls. The results indicate that metabolism of SM triggered by neutral SMase participates in ethanol-induced apoptosis in Hep G2 cells and activation of caspase-3 is involved in the apoptotic pathway. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Alcohol and Alcoholism
volume
35
issue
6
pages
569 - 573
publisher
Oxford University Press
external identifiers
  • pmid:11093963
  • scopus:0033662390
ISSN
1464-3502
language
English
LU publication?
yes
id
336608cb-9501-4b49-9e0f-90d7371cf192 (old id 1117909)
alternative location
http://alcalc.oxfordjournals.org/cgi/content/abstract/35/6/569
date added to LUP
2008-06-24 14:44:21
date last changed
2017-10-22 03:51:18
@article{336608cb-9501-4b49-9e0f-90d7371cf192,
  abstract     = {The mechanism underlying ethanol-induced apoptosis in liver cells is not clear. Sphingomyelin (SM) metabolism is a novel signal transduction pathway that has an impact on apoptosis in many cell types. We investigated whether the SM pathway is involved in ethanol-induced apoptosis in the liver. Hep G2 cells were treated with ethanol followed by assaying apoptosis, sphingomyelinase (SMase) activity, caspase-3 activity, and the changes of SM content in the cells. We found that ethanol dose-dependently increased apoptosis and the effect was accompanied by increases of caspase-3 activity and neutral SMase activity. At concentrations of 80 and 160 mM, ethanol significantly increased caspase-3 activity by 120% and neutral SMase activity by 24%. The activity of acid SMase was only slightly increased without statistical significance. C(2)-ceramide, the exogenous SM metabolite, mimicked the effects of ethanol on apoptosis and caspase-3 activation. When the SM content was determined 24 h after treatment with ethanol, its level was 15% lower than that of controls. The results indicate that metabolism of SM triggered by neutral SMase participates in ethanol-induced apoptosis in Hep G2 cells and activation of caspase-3 is involved in the apoptotic pathway.},
  author       = {Liu, J J and Wang, J Y and Hertervig, Erik and Cheng, Yajun and Nilsson, Åke and Duan, Rui-Dong},
  issn         = {1464-3502},
  language     = {eng},
  number       = {6},
  pages        = {569--573},
  publisher    = {Oxford University Press},
  series       = {Alcohol and Alcoholism},
  title        = {Activation of neutral sphingomyelinase participates in ethanol-induced apoptosis in Hep G2 cells},
  volume       = {35},
  year         = {2000},
}