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Airborne cell analysis

Santesson, Sabina LU ; Andersson, Martin; Degerman, Eva LU ; Johansson, Thomas LU ; Nilsson, Johan LU and Nilsson, Staffan LU (2000) In Analytical Chemistry 72(15). p.3412-3418
Abstract
A miniaturized analysis system for the study of living cells and biochemical reactions in microdroplets was developed. The technique utilizes an in-house-developed piezoelectric flow-through droplet dispenser for precise reagent supply and an ultrasonic levitator for contactless sample handling. A few-cell study was performed with living primary adipocytes. Droplets (500 nL) containing 3-15 individual cells were acoustically levitated. The addition of beta-adrenergic agonists into the levitated droplet using the droplet dispenser stimulated adipocyte lipolysis, leading to free fatty acid release and a consequent pH decrease of the surrounding buffer. The addition of insulin antagonized lipolysis and hence also the decrease in pH. The... (More)
A miniaturized analysis system for the study of living cells and biochemical reactions in microdroplets was developed. The technique utilizes an in-house-developed piezoelectric flow-through droplet dispenser for precise reagent supply and an ultrasonic levitator for contactless sample handling. A few-cell study was performed with living primary adipocytes. Droplets (500 nL) containing 3-15 individual cells were acoustically levitated. The addition of beta-adrenergic agonists into the levitated droplet using the droplet dispenser stimulated adipocyte lipolysis, leading to free fatty acid release and a consequent pH decrease of the surrounding buffer. The addition of insulin antagonized lipolysis and hence also the decrease in pH. The changes in pH, i.e., the cell response in the droplet, were followed using a pH-dependent fluorophore continuously monitored by fluorescence imaging detection. An image analysis computer program was employed to calculate the droplet intensities. To counteract droplet evaporation, found to affect the fluorescence intensities, a separate dispenser was used to continually add water, thus keeping the droplet volume constant. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
72
issue
15
pages
3412 - 3418
publisher
The American Chemical Society
external identifiers
  • pmid:10952520
  • scopus:0034254832
ISSN
1520-6882
DOI
10.1021/ac000140r
language
English
LU publication?
yes
id
b0fd5003-5ff2-4d09-a263-fb2b310b4cf7 (old id 1117953)
date added to LUP
2008-06-25 08:14:58
date last changed
2017-01-01 04:49:15
@article{b0fd5003-5ff2-4d09-a263-fb2b310b4cf7,
  abstract     = {A miniaturized analysis system for the study of living cells and biochemical reactions in microdroplets was developed. The technique utilizes an in-house-developed piezoelectric flow-through droplet dispenser for precise reagent supply and an ultrasonic levitator for contactless sample handling. A few-cell study was performed with living primary adipocytes. Droplets (500 nL) containing 3-15 individual cells were acoustically levitated. The addition of beta-adrenergic agonists into the levitated droplet using the droplet dispenser stimulated adipocyte lipolysis, leading to free fatty acid release and a consequent pH decrease of the surrounding buffer. The addition of insulin antagonized lipolysis and hence also the decrease in pH. The changes in pH, i.e., the cell response in the droplet, were followed using a pH-dependent fluorophore continuously monitored by fluorescence imaging detection. An image analysis computer program was employed to calculate the droplet intensities. To counteract droplet evaporation, found to affect the fluorescence intensities, a separate dispenser was used to continually add water, thus keeping the droplet volume constant.},
  author       = {Santesson, Sabina and Andersson, Martin and Degerman, Eva and Johansson, Thomas and Nilsson, Johan and Nilsson, Staffan},
  issn         = {1520-6882},
  language     = {eng},
  number       = {15},
  pages        = {3412--3418},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Airborne cell analysis},
  url          = {http://dx.doi.org/10.1021/ac000140r},
  volume       = {72},
  year         = {2000},
}