The amino-terminal part of PRELP binds to heparin and heparan sulfate
(2000) In Journal of Biological Chemistry 275(52). p.40695-40702- Abstract
- PRELP (proline, arginine-rich end leucine-rich repeat protein) is an extracellular matrix leucine-rich repeat protein. The amino-terminal region of PRELP differs from that of other leucine-rich repeat proteins in containing a high number of proline and arginine residues. The clustered proline and basic residues are conserved in rat, bovine, and human PRELP. Although the function of PRELP is not yet known, the clustered arginine residues suggest a heparan sulfate/heparin-binding capacity. We show here that PRELP indeed binds heparin and heparan sulfate. Truncated PRELP without the amino-terminal region does not bind heparin. The dissociation constant for the interaction of PRELP with heparin was determined by an in solution binding assay... (More)
- PRELP (proline, arginine-rich end leucine-rich repeat protein) is an extracellular matrix leucine-rich repeat protein. The amino-terminal region of PRELP differs from that of other leucine-rich repeat proteins in containing a high number of proline and arginine residues. The clustered proline and basic residues are conserved in rat, bovine, and human PRELP. Although the function of PRELP is not yet known, the clustered arginine residues suggest a heparan sulfate/heparin-binding capacity. We show here that PRELP indeed binds heparin and heparan sulfate. Truncated PRELP without the amino-terminal region does not bind heparin. The dissociation constant for the interaction of PRELP with heparin was determined by an in solution binding assay and by surface plasmon resonance analysis to be in the range of 10-30 nm. A 6-mer heparin oligosaccharide was the smallest size showing binding to PRELP. The binding increased with increasing length up to an 18-mer and depended on the degree of sulfation of heparin as well as heparan sulfate. Sulfate groups at all positions were shown to be of importance for the binding. Fibroblasts bind PRELP, and this interaction is inhibited with heparin, suggesting a function for PRELP as a linker between the matrix and cell surface proteoglycans. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1118185
- author
- Bengtsson, Eva
; Aspberg, Anders
LU
; Heinegård, Dick LU ; Sommarin, Y and Spillmann, D
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 275
- issue
- 52
- pages
- 40695 - 40702
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- pmid:11007795
- scopus:0034731438
- ISSN
- 1083-351X
- DOI
- 10.1074/jbc.M007917200
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151)
- id
- f2a912cc-7459-49dd-a4e8-d844e3969352 (old id 1118185)
- date added to LUP
- 2016-04-01 12:25:04
- date last changed
- 2022-01-27 03:27:28
@article{f2a912cc-7459-49dd-a4e8-d844e3969352, abstract = {{PRELP (proline, arginine-rich end leucine-rich repeat protein) is an extracellular matrix leucine-rich repeat protein. The amino-terminal region of PRELP differs from that of other leucine-rich repeat proteins in containing a high number of proline and arginine residues. The clustered proline and basic residues are conserved in rat, bovine, and human PRELP. Although the function of PRELP is not yet known, the clustered arginine residues suggest a heparan sulfate/heparin-binding capacity. We show here that PRELP indeed binds heparin and heparan sulfate. Truncated PRELP without the amino-terminal region does not bind heparin. The dissociation constant for the interaction of PRELP with heparin was determined by an in solution binding assay and by surface plasmon resonance analysis to be in the range of 10-30 nm. A 6-mer heparin oligosaccharide was the smallest size showing binding to PRELP. The binding increased with increasing length up to an 18-mer and depended on the degree of sulfation of heparin as well as heparan sulfate. Sulfate groups at all positions were shown to be of importance for the binding. Fibroblasts bind PRELP, and this interaction is inhibited with heparin, suggesting a function for PRELP as a linker between the matrix and cell surface proteoglycans.}}, author = {{Bengtsson, Eva and Aspberg, Anders and Heinegård, Dick and Sommarin, Y and Spillmann, D}}, issn = {{1083-351X}}, language = {{eng}}, number = {{52}}, pages = {{40695--40702}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Biological Chemistry}}, title = {{The amino-terminal part of PRELP binds to heparin and heparan sulfate}}, url = {{http://dx.doi.org/10.1074/jbc.M007917200}}, doi = {{10.1074/jbc.M007917200}}, volume = {{275}}, year = {{2000}}, }