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Dose-effect relationship of apoptosis induced by fission-neutron in murine thymocytes

Yuan, Bin ; Li, Liang ; Xue, Wenchang ; Sun, Jianmin LU and Wang, Baoqin (2000) In Chinese Journal of Radiological Medical Protection 20. p.87-87
Abstract
Objective　To investigate the effectiveness of high LET fission-neutron to induce apoptosis in murine thymocytes and to compare it with that of low LET 60Co γ-ray. Methods Apoptosis induction was studied qualitatively by light and transmission electron microscopy and DNA gel electrophoresis,also quantitatively by flow cytometry(FCM) and diphenylamine (DPA)methods. Results DNA ladders of murine thymocytes were detectable,the typical apoptosis of thymocytes could be observed morphologically by means of light and electron microscopy at 6 h after fission-neutron irradiation with doses ranging from 0.5 to 5.0 Gy,meanwhile the percentages of apoptosis increased with increasing doses.After exposure to γ-rays with doses ranging from 1.0 to 30... (More)
Objective　To investigate the effectiveness of high LET fission-neutron to induce apoptosis in murine thymocytes and to compare it with that of low LET 60Co γ-ray. Methods Apoptosis induction was studied qualitatively by light and transmission electron microscopy and DNA gel electrophoresis,also quantitatively by flow cytometry(FCM) and diphenylamine (DPA)methods. Results DNA ladders of murine thymocytes were detectable,the typical apoptosis of thymocytes could be observed morphologically by means of light and electron microscopy at 6 h after fission-neutron irradiation with doses ranging from 0.5 to 5.0 Gy,meanwhile the percentages of apoptosis increased with increasing doses.After exposure to γ-rays with doses ranging from 1.0 to 30 Gy,the expermiental results were similar to those from neutron radiation.The incidence of apoptosis peaked at about 20 Gy，the percentages did not increase further when doses increased. Conclusion Apoptosis of murine thymocytes can be induced when mice are exposed to either fission-neutron (0.5-5.0 Gy) or to γ-ray (1-30 Gy).Although the relationship between apoptosis and radiation doses is similar,the percentage of apoptosis induced by neutron irradiation is higher than that induced by γ-irradiation.The RBE values of fission-neutron for inducing apoptosis murine thymocytes are 2.09 (by FCM method) and 2.37 (by DPA method),respectively.These results also suggest that fission-neutron-induced murine immune tissue is more severe than that induced by γ-rays at several hours post-irradiation and this might be the basis for heavy damage to immune tissues induced by fission-neutron-irradiation in later period. (Less)
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Chinese Journal of Radiological Medical Protection
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20
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87 - 87
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English
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The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Experimental Clinical Chemistry (013016010)
id
255e8063-6eb4-425b-943d-f4cc0b5fb829 (old id 1118417)
alternative location
http://en.cnki.com.cn/Article_en/CJFDTotal-ZHFS200002007.htm
date added to LUP
2016-04-04 14:23:39
date last changed
2018-11-21 21:20:05
@article{255e8063-6eb4-425b-943d-f4cc0b5fb829,
  abstract     = {{Objective　To investigate the effectiveness of high LET fission-neutron to induce apoptosis in murine thymocytes and to compare it with that of low LET 60Co γ-ray. Methods Apoptosis induction was studied qualitatively by light and transmission electron microscopy and DNA gel electrophoresis,also quantitatively by flow cytometry(FCM) and diphenylamine (DPA)methods. Results DNA ladders of murine thymocytes were detectable,the typical apoptosis of thymocytes could be observed morphologically by means of light and electron microscopy at 6 h after fission-neutron irradiation with doses ranging from 0.5 to 5.0 Gy,meanwhile the percentages of apoptosis increased with increasing doses.After exposure to γ-rays with doses ranging from 1.0 to 30 Gy,the expermiental results were similar to those from neutron radiation.The incidence of apoptosis peaked at about 20 Gy，the percentages did not increase further when doses increased. Conclusion Apoptosis of murine thymocytes can be induced when mice are exposed to either fission-neutron (0.5-5.0 Gy) or to γ-ray (1-30 Gy).Although the relationship between apoptosis and radiation doses is similar,the percentage of apoptosis induced by neutron irradiation is higher than that induced by γ-irradiation.The RBE values of fission-neutron for inducing apoptosis murine thymocytes are 2.09 (by FCM method) and 2.37 (by DPA method),respectively.These results also suggest that fission-neutron-induced murine immune tissue is more severe than that induced by γ-rays at several hours post-irradiation and this might be the basis for heavy damage to immune tissues induced by fission-neutron-irradiation in later period.}},
  author       = {{Yuan, Bin and Li, Liang and Xue, Wenchang and Sun, Jianmin and Wang, Baoqin}},
  language     = {{eng}},
  pages        = {{87--87}},
  series       = {{Chinese Journal of Radiological Medical Protection}},
  title        = {{Dose-effect relationship of apoptosis induced by fission-neutron in murine thymocytes}},
  url          = {{http://en.cnki.com.cn/Article_en/CJFDTotal-ZHFS200002007.htm}},
  volume       = {{20}},
  year         = {{2000}},
}