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Identification of the major proteoglycans from human myometrium

Hjelm, Ann; Ekman-Ordeberg, Gunvor; Barchan, Karin and Malmström, Anders LU (2001) In Acta Obstetricia et Gynecologica Scandinavica 80(12). p.1084-1090
Abstract
Background. During pregnancy and parturition a remodeling within the extracellular matrix of the cervix and the corpus uteri occurs, which is of fundamental importance to a normal labor. The aim of this study is to identify the major proteoglycans in corpus uteri of non-pregnant subjects. Methods. From human uterine tissue proteoglycans were extracted and purified using CsCl-density gradient centrifugation, gel and ion-exchange chromatography. The proteoglycans were quantified and identified by Alcian Blue before and after ABC-digestion and by Western blotting. Results. The results showed that the corpus uteri contains a substantial amount of proteoglycans, 1.825 mug/mg wet weight. Decorin is dominating, constituting 63% of the total... (More)
Background. During pregnancy and parturition a remodeling within the extracellular matrix of the cervix and the corpus uteri occurs, which is of fundamental importance to a normal labor. The aim of this study is to identify the major proteoglycans in corpus uteri of non-pregnant subjects. Methods. From human uterine tissue proteoglycans were extracted and purified using CsCl-density gradient centrifugation, gel and ion-exchange chromatography. The proteoglycans were quantified and identified by Alcian Blue before and after ABC-digestion and by Western blotting. Results. The results showed that the corpus uteri contains a substantial amount of proteoglycans, 1.825 mug/mg wet weight. Decorin is dominating, constituting 63% of the total amount of proteoglycans. Heparan sulphate proteoglycans accounted for 20% and biglycan for 16%. Less than 1% consisted of the large proteoglycan versican. Conclusions. Further investigations must be performed to provide more information of the biological role of the proteoglycans in the uterus, especially during labor, by the presence of heparan sulphate proteoglycans and the minute presence of versican which indicate that the proteoglycan composition and organization is different to that of the cervix. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
uterus, heparan sulphate proteoglycan, decorin, biglycan
in
Acta Obstetricia et Gynecologica Scandinavica
volume
80
issue
12
pages
1084 - 1090
publisher
Wiley-Blackwell
external identifiers
  • wos:000173282600002
  • scopus:0035695796
ISSN
1600-0412
DOI
10.1034/j.1600-0412.2001.801202.x
language
English
LU publication?
yes
id
4f84cc1d-3b31-49b8-8bda-921019016231 (old id 1118518)
date added to LUP
2008-06-30 14:16:54
date last changed
2018-05-29 11:30:08
@article{4f84cc1d-3b31-49b8-8bda-921019016231,
  abstract     = {Background. During pregnancy and parturition a remodeling within the extracellular matrix of the cervix and the corpus uteri occurs, which is of fundamental importance to a normal labor. The aim of this study is to identify the major proteoglycans in corpus uteri of non-pregnant subjects. Methods. From human uterine tissue proteoglycans were extracted and purified using CsCl-density gradient centrifugation, gel and ion-exchange chromatography. The proteoglycans were quantified and identified by Alcian Blue before and after ABC-digestion and by Western blotting. Results. The results showed that the corpus uteri contains a substantial amount of proteoglycans, 1.825 mug/mg wet weight. Decorin is dominating, constituting 63% of the total amount of proteoglycans. Heparan sulphate proteoglycans accounted for 20% and biglycan for 16%. Less than 1% consisted of the large proteoglycan versican. Conclusions. Further investigations must be performed to provide more information of the biological role of the proteoglycans in the uterus, especially during labor, by the presence of heparan sulphate proteoglycans and the minute presence of versican which indicate that the proteoglycan composition and organization is different to that of the cervix.},
  author       = {Hjelm, Ann and Ekman-Ordeberg, Gunvor and Barchan, Karin and Malmström, Anders},
  issn         = {1600-0412},
  keyword      = {uterus,heparan sulphate proteoglycan,decorin,biglycan},
  language     = {eng},
  number       = {12},
  pages        = {1084--1090},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Obstetricia et Gynecologica Scandinavica},
  title        = {Identification of the major proteoglycans from human myometrium},
  url          = {http://dx.doi.org/10.1034/j.1600-0412.2001.801202.x},
  volume       = {80},
  year         = {2001},
}