Identification of the major proteoglycans from human myometrium
Hjelm, Ann ; Ekman-Ordeberg, Gunvor ; Barchan, Karin and Malmström, Anders LU
(2001)
In Acta Obstetricia et Gynecologica Scandinavica
80(12).
p.1084-1090
- Abstract
- Background. During pregnancy and parturition a remodeling within the extracellular matrix of the cervix and the corpus uteri occurs, which is of fundamental importance to a normal labor. The aim of this study is to identify the major proteoglycans in corpus uteri of non-pregnant subjects. Methods. From human uterine tissue proteoglycans were extracted and purified using CsCl-density gradient centrifugation, gel and ion-exchange chromatography. The proteoglycans were quantified and identified by Alcian Blue before and after ABC-digestion and by Western blotting. Results. The results showed that the corpus uteri contains a substantial amount of proteoglycans, 1.825 mug/mg wet weight. Decorin is dominating, constituting 63% of the total... (More)
- Background. During pregnancy and parturition a remodeling within the extracellular matrix of the cervix and the corpus uteri occurs, which is of fundamental importance to a normal labor. The aim of this study is to identify the major proteoglycans in corpus uteri of non-pregnant subjects. Methods. From human uterine tissue proteoglycans were extracted and purified using CsCl-density gradient centrifugation, gel and ion-exchange chromatography. The proteoglycans were quantified and identified by Alcian Blue before and after ABC-digestion and by Western blotting. Results. The results showed that the corpus uteri contains a substantial amount of proteoglycans, 1.825 mug/mg wet weight. Decorin is dominating, constituting 63% of the total amount of proteoglycans. Heparan sulphate proteoglycans accounted for 20% and biglycan for 16%. Less than 1% consisted of the large proteoglycan versican. Conclusions. Further investigations must be performed to provide more information of the biological role of the proteoglycans in the uterus, especially during labor, by the presence of heparan sulphate proteoglycans and the minute presence of versican which indicate that the proteoglycan composition and organization is different to that of the cervix. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1118518
- author
- Hjelm, Ann
; Ekman-Ordeberg, Gunvor
; Barchan, Karin
and Malmström, Anders
LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- uterus, heparan sulphate proteoglycan, decorin, biglycan
- in
- Acta Obstetricia et Gynecologica Scandinavica
- volume
- 80
- issue
- 12
- pages
- 1084 - 1090
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000173282600002
- scopus:0035695796
- ISSN
- 1600-0412
- DOI
- 10.1034/j.1600-0412.2001.801202.x
- language
- English
- LU publication?
- yes
- id
- 4f84cc1d-3b31-49b8-8bda-921019016231 (old id 1118518)
- date added to LUP
- 2016-04-01 15:29:02
- date last changed
- 2022-01-28 05:32:41
@article{4f84cc1d-3b31-49b8-8bda-921019016231,
abstract = {{Background. During pregnancy and parturition a remodeling within the extracellular matrix of the cervix and the corpus uteri occurs, which is of fundamental importance to a normal labor. The aim of this study is to identify the major proteoglycans in corpus uteri of non-pregnant subjects. Methods. From human uterine tissue proteoglycans were extracted and purified using CsCl-density gradient centrifugation, gel and ion-exchange chromatography. The proteoglycans were quantified and identified by Alcian Blue before and after ABC-digestion and by Western blotting. Results. The results showed that the corpus uteri contains a substantial amount of proteoglycans, 1.825 mug/mg wet weight. Decorin is dominating, constituting 63% of the total amount of proteoglycans. Heparan sulphate proteoglycans accounted for 20% and biglycan for 16%. Less than 1% consisted of the large proteoglycan versican. Conclusions. Further investigations must be performed to provide more information of the biological role of the proteoglycans in the uterus, especially during labor, by the presence of heparan sulphate proteoglycans and the minute presence of versican which indicate that the proteoglycan composition and organization is different to that of the cervix.}},
author = {{Hjelm, Ann and Ekman-Ordeberg, Gunvor and Barchan, Karin and Malmström, Anders}},
issn = {{1600-0412}},
keywords = {{uterus; heparan sulphate proteoglycan; decorin; biglycan}},
language = {{eng}},
number = {{12}},
pages = {{1084--1090}},
publisher = {{Wiley-Blackwell}},
series = {{Acta Obstetricia et Gynecologica Scandinavica}},
title = {{Identification of the major proteoglycans from human myometrium}},
url = {{http://dx.doi.org/10.1034/j.1600-0412.2001.801202.x}},
doi = {{10.1034/j.1600-0412.2001.801202.x}},
volume = {{80}},
year = {{2001}},
}