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Different effect on axonal outgrowth of application of nonabsorbable or absorbable tubes around a nerve repair

Danielsson, Pär A.; Adolfsson, Lars and Dahlin, Lars LU (2001) In Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00 35(4). p.347-353
Abstract
We studied regeneration distance of rat sciatic nerve, with the sensory pinch reflex test and immunocytochemical staining for neurofilaments, four to 21 days after transsection, repair, and enclosure of the repair site in either a non-absorbable silicone tube or an absorbable polyglycolic acid (PGA) tube. The size of both tube-types was carefully selected so that they did not compress the repaired nerve. The opposite nerve was repaired and not inserted in a tube (control). The regeneration distances in repaired nerves enclosed in silicone tube were significantly longer than the control side at all time points, a result not seen when PGA tube was used. The number of proliferating non-neuronal cells (incorporation of 5-bromodeoxyuridine... (More)
We studied regeneration distance of rat sciatic nerve, with the sensory pinch reflex test and immunocytochemical staining for neurofilaments, four to 21 days after transsection, repair, and enclosure of the repair site in either a non-absorbable silicone tube or an absorbable polyglycolic acid (PGA) tube. The size of both tube-types was carefully selected so that they did not compress the repaired nerve. The opposite nerve was repaired and not inserted in a tube (control). The regeneration distances in repaired nerves enclosed in silicone tube were significantly longer than the control side at all time points, a result not seen when PGA tube was used. The number of proliferating non-neuronal cells (incorporation of 5-bromodeoxyuridine (BrdU)) was studied just proximal to the site of nerve repair after six days. Numerous stained cells were seen, but there where no significant differences between the groups. We conclude that outgrowth of sensory axons after transsection and repair of rat sciatic nerve with sutures can be increased by enclosing the site of repair in a silicone tube but not in a PGA tube. The effect is probably not related to the number of proliferative non-neuronal cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Regeneration, Nerve, Sensory, Fibre, Silicone, Tube, Polyglycolic, Acid, Pga, Macrophages, 5-BROMODEOXYURIDINE, Brdu, Schwann, Cells, Neurofilaments
in
Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00
volume
35
issue
4
pages
347 - 353
publisher
Taylor & Francis
external identifiers
  • wos:000173155800002
  • scopus:0035666463
ISSN
1651-2073
DOI
10.1080/028443101317149309
language
English
LU publication?
yes
id
46f88840-1eae-44fb-8cea-4da52cb34955 (old id 1118528)
date added to LUP
2008-06-26 11:31:52
date last changed
2018-05-29 09:59:33
@article{46f88840-1eae-44fb-8cea-4da52cb34955,
  abstract     = {We studied regeneration distance of rat sciatic nerve, with the sensory pinch reflex test and immunocytochemical staining for neurofilaments, four to 21 days after transsection, repair, and enclosure of the repair site in either a non-absorbable silicone tube or an absorbable polyglycolic acid (PGA) tube. The size of both tube-types was carefully selected so that they did not compress the repaired nerve. The opposite nerve was repaired and not inserted in a tube (control). The regeneration distances in repaired nerves enclosed in silicone tube were significantly longer than the control side at all time points, a result not seen when PGA tube was used. The number of proliferating non-neuronal cells (incorporation of 5-bromodeoxyuridine (BrdU)) was studied just proximal to the site of nerve repair after six days. Numerous stained cells were seen, but there where no significant differences between the groups. We conclude that outgrowth of sensory axons after transsection and repair of rat sciatic nerve with sutures can be increased by enclosing the site of repair in a silicone tube but not in a PGA tube. The effect is probably not related to the number of proliferative non-neuronal cells.},
  author       = {Danielsson, Pär A. and Adolfsson, Lars and Dahlin, Lars},
  issn         = {1651-2073},
  keyword      = {Regeneration,Nerve,Sensory,Fibre,Silicone,Tube,Polyglycolic,Acid,Pga,Macrophages,5-BROMODEOXYURIDINE,Brdu,Schwann,Cells,Neurofilaments},
  language     = {eng},
  number       = {4},
  pages        = {347--353},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00},
  title        = {Different effect on axonal outgrowth of application of nonabsorbable or absorbable tubes around a nerve repair},
  url          = {http://dx.doi.org/10.1080/028443101317149309},
  volume       = {35},
  year         = {2001},
}