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Fatty acid specificity of hormone-sensitive lipase: implication in the selective hydrolysis of triacylglycerols

Raclot, Thierry; Holm, Cecilia LU and Langin, Dominique (2001) In Journal of Lipid Research 42(12). p.2049-2057
Abstract
The selective mobilization of fatty acids from white fat cells depends on their molecular structure, in particular the degree of unsaturation. The present study was designed to examine if the release of fatty acids by hormone-sensitive lipase (HSL) in vitro i) is influenced by the amount of unsaturation, ii) depends on the temperature, and iii) could explain the selective pattern of fatty acid mobilization and notably the preferential mobilization of certain highly unsaturated fatty acids. Recombinant rat and human HSL were incubated with a lipid emulsion. The hydrolysis of 35 individual fatty acids, ranging in chain length from 12 to 24 carbon atoms and in unsaturation from 0 to 6 double bonds was measured. Fatty acid composition of in... (More)
The selective mobilization of fatty acids from white fat cells depends on their molecular structure, in particular the degree of unsaturation. The present study was designed to examine if the release of fatty acids by hormone-sensitive lipase (HSL) in vitro i) is influenced by the amount of unsaturation, ii) depends on the temperature, and iii) could explain the selective pattern of fatty acid mobilization and notably the preferential mobilization of certain highly unsaturated fatty acids. Recombinant rat and human HSL were incubated with a lipid emulsion. The hydrolysis of 35 individual fatty acids, ranging in chain length from 12 to 24 carbon atoms and in unsaturation from 0 to 6 double bonds was measured. Fatty acid composition of in vitro released NEFA was compared with that of fat cell triacylglycerols (TAG), the ratio % NEFA/% TAG being defined as the relative hydrolysis. The relative hydrolysis of individual fatty acids differed widely, ranging from 0.44 (24:ln-9) to 1.49 (18:ln-7) with rat HSL, and from 0.38 (24:ln-9) to 1.67 (18:ln-7) with human HSL. No major difference was observed between rat and human HSL. The relative release was dependent on the number of double bonds according to chain length. The amount of fatty acid released by recombinant rat HSL was decreased but remained robust at 4 degreesC compared with 37 degreesC, and the relative hydrolysis of some individual fatty acids was affected. The relative hydrolysis of fatty acids moderately, weakly, and highly mobilized by adipose tissue in vivo was similar and close to unity in vitro. We conclude that i) the release of fatty acids by HSL is only slightly affected by their degree of unsaturation, ii) the ability of HSL to efficiently and selectively release fatty acids at low temperature could reflect a cold adaptability for poikilotherms or hibernators when endogenous lipids are needed, and iii) the selectivity of fatty acid hydrolysis by HSL does not fully account for the selective pattern of fatty acid mobilization, but could contribute to explain the preferential mobilization of some highly unsaturated fatty acids compared with others. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
lipolysis, fatty acid molecular structure, lipid emulsion, adipose tissue
in
Journal of Lipid Research
volume
42
issue
12
pages
2049 - 2057
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • wos:000172819800014
  • scopus:0035658279
ISSN
1539-7262
language
English
LU publication?
yes
id
26041ae8-8a19-49a3-8248-6d2b25012854 (old id 1118546)
alternative location
http://www.jlr.org/cgi/content/full/42/12/2049
date added to LUP
2008-07-10 10:42:16
date last changed
2018-05-29 09:33:29
@article{26041ae8-8a19-49a3-8248-6d2b25012854,
  abstract     = {The selective mobilization of fatty acids from white fat cells depends on their molecular structure, in particular the degree of unsaturation. The present study was designed to examine if the release of fatty acids by hormone-sensitive lipase (HSL) in vitro i) is influenced by the amount of unsaturation, ii) depends on the temperature, and iii) could explain the selective pattern of fatty acid mobilization and notably the preferential mobilization of certain highly unsaturated fatty acids. Recombinant rat and human HSL were incubated with a lipid emulsion. The hydrolysis of 35 individual fatty acids, ranging in chain length from 12 to 24 carbon atoms and in unsaturation from 0 to 6 double bonds was measured. Fatty acid composition of in vitro released NEFA was compared with that of fat cell triacylglycerols (TAG), the ratio % NEFA/% TAG being defined as the relative hydrolysis. The relative hydrolysis of individual fatty acids differed widely, ranging from 0.44 (24:ln-9) to 1.49 (18:ln-7) with rat HSL, and from 0.38 (24:ln-9) to 1.67 (18:ln-7) with human HSL. No major difference was observed between rat and human HSL. The relative release was dependent on the number of double bonds according to chain length. The amount of fatty acid released by recombinant rat HSL was decreased but remained robust at 4 degreesC compared with 37 degreesC, and the relative hydrolysis of some individual fatty acids was affected. The relative hydrolysis of fatty acids moderately, weakly, and highly mobilized by adipose tissue in vivo was similar and close to unity in vitro. We conclude that i) the release of fatty acids by HSL is only slightly affected by their degree of unsaturation, ii) the ability of HSL to efficiently and selectively release fatty acids at low temperature could reflect a cold adaptability for poikilotherms or hibernators when endogenous lipids are needed, and iii) the selectivity of fatty acid hydrolysis by HSL does not fully account for the selective pattern of fatty acid mobilization, but could contribute to explain the preferential mobilization of some highly unsaturated fatty acids compared with others.},
  author       = {Raclot, Thierry and Holm, Cecilia and Langin, Dominique},
  issn         = {1539-7262},
  keyword      = {lipolysis,fatty acid molecular structure,lipid emulsion,adipose tissue},
  language     = {eng},
  number       = {12},
  pages        = {2049--2057},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Journal of Lipid Research},
  title        = {Fatty acid specificity of hormone-sensitive lipase: implication in the selective hydrolysis of triacylglycerols},
  volume       = {42},
  year         = {2001},
}