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The effect of exercise training on hormone-sensitive lipase in rat intra-abdominal adipose tissue and muscle

Enevoldsen, L.H.; Stallknecht, B.; Langfort, J.; Petersen, L.N.; Holm, Cecilia LU ; Ploug, T. and Galbo, H. (2001) In Journal of Physiology 536(3). p.871-877
Abstract
1. Adrenaline-stimulated lipolysis in adipose tissue may increase with training. The rate-limiting step in adipose tissue lipolysis is catalysed by the enzyme hormone-sensitive lipase (HSL). We studied the effect of exercise training on the activity of the total and the activated form of HSL, referred to as HSL (DG) and HSL (TG), respectively, and on the concentration of HSL protein in retroperitoneal (RE) and mesenteric (ME) adipose tissue, and in the extensor digitorum. longus (EDL) and soleus muscles in rats. 2. Rats (weighing 96 + 1 g, mean +/- S.E.M.) were either swim trained (T, 18 weeks, n = 12) or sedentary (S, n = 12). Then RE and ME adipose tissue and the EDL and soleus muscles were incubated for 20 min with 4.4 muM adrenaline.... (More)
1. Adrenaline-stimulated lipolysis in adipose tissue may increase with training. The rate-limiting step in adipose tissue lipolysis is catalysed by the enzyme hormone-sensitive lipase (HSL). We studied the effect of exercise training on the activity of the total and the activated form of HSL, referred to as HSL (DG) and HSL (TG), respectively, and on the concentration of HSL protein in retroperitoneal (RE) and mesenteric (ME) adipose tissue, and in the extensor digitorum. longus (EDL) and soleus muscles in rats. 2. Rats (weighing 96 + 1 g, mean +/- S.E.M.) were either swim trained (T, 18 weeks, n = 12) or sedentary (S, n = 12). Then RE and ME adipose tissue and the EDL and soleus muscles were incubated for 20 min with 4.4 muM adrenaline. 3. HSL enzyme activities in adipose tissue were higher in T compared with S rats. Furthermore, in RE adipose tissue, training also doubled HSL protein concentration (P < 0.05). In ME adipose tissue, the HSL protein levels did not differ significantly between T and S rats. In muscle, HSL (TG) activity as well as HSL (TG)/HSL (DG) were. lower in T rats, whereas HSL (DG) activity did not differ between groups. Furthermore, HSL protein concentration in muscle did not differ between T and S rats (P > 0.05). 4. In conclusion, training increased the amount of HSL and the sensitivity of HSL to stimulation by adrenaline in intra-abdominal adipose tissue, the extent of the change differing between anatomical locations. In contrast, in skeletal muscle the amount of HSL was unchanged and its sensitivity to stimulation by adrenaline reduced after training. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Physiology
volume
536
issue
3
pages
871 - 877
publisher
The Physiological Society
external identifiers
  • wos:000172240300018
  • scopus:0035498538
ISSN
1469-7793
DOI
10.1111/j.1469-7793.2001.t01-1-00871.x
language
English
LU publication?
yes
id
5d00a36e-5657-43a8-bf19-7c6ccad88d77 (old id 1118696)
date added to LUP
2008-06-26 15:59:31
date last changed
2018-01-07 08:58:56
@article{5d00a36e-5657-43a8-bf19-7c6ccad88d77,
  abstract     = {1. Adrenaline-stimulated lipolysis in adipose tissue may increase with training. The rate-limiting step in adipose tissue lipolysis is catalysed by the enzyme hormone-sensitive lipase (HSL). We studied the effect of exercise training on the activity of the total and the activated form of HSL, referred to as HSL (DG) and HSL (TG), respectively, and on the concentration of HSL protein in retroperitoneal (RE) and mesenteric (ME) adipose tissue, and in the extensor digitorum. longus (EDL) and soleus muscles in rats. 2. Rats (weighing 96 + 1 g, mean +/- S.E.M.) were either swim trained (T, 18 weeks, n = 12) or sedentary (S, n = 12). Then RE and ME adipose tissue and the EDL and soleus muscles were incubated for 20 min with 4.4 muM adrenaline. 3. HSL enzyme activities in adipose tissue were higher in T compared with S rats. Furthermore, in RE adipose tissue, training also doubled HSL protein concentration (P &lt; 0.05). In ME adipose tissue, the HSL protein levels did not differ significantly between T and S rats. In muscle, HSL (TG) activity as well as HSL (TG)/HSL (DG) were. lower in T rats, whereas HSL (DG) activity did not differ between groups. Furthermore, HSL protein concentration in muscle did not differ between T and S rats (P &gt; 0.05). 4. In conclusion, training increased the amount of HSL and the sensitivity of HSL to stimulation by adrenaline in intra-abdominal adipose tissue, the extent of the change differing between anatomical locations. In contrast, in skeletal muscle the amount of HSL was unchanged and its sensitivity to stimulation by adrenaline reduced after training.},
  author       = {Enevoldsen, L.H. and Stallknecht, B. and Langfort, J. and Petersen, L.N. and Holm, Cecilia and Ploug, T. and Galbo, H.},
  issn         = {1469-7793},
  language     = {eng},
  number       = {3},
  pages        = {871--877},
  publisher    = {The Physiological Society},
  series       = {Journal of Physiology},
  title        = {The effect of exercise training on hormone-sensitive lipase in rat intra-abdominal adipose tissue and muscle},
  url          = {http://dx.doi.org/10.1111/j.1469-7793.2001.t01-1-00871.x},
  volume       = {536},
  year         = {2001},
}