Advanced

Comparative genomic hybridization of postirradiation sarcomas

Tarkkanen, Maija; Wiklund, Tom A.; Virolainen, Martti J.; Larramendy, Marcelo L.; Mandahl, Nils LU ; Mertens, Fredrik LU ; Blomqvist, Carl P.; Tukiainen, Erkki J.; Miettinen, Markku M. A. and Elomaa, A. Inkeri, et al. (2001) In Cancer 92(7). p.1992-1998
Abstract
BACKGROUND. Radiotherapy is a known risk factor for sarcoma development. Postirradiation sarcomas arise within the radiation field after a latency period of several years and usually are highly malignant. Very little is yet known about their genetic changes. METHODS. Twenty-seven postirradiation sarcomas were analyzed by comparative genomic hybridization, which allows genome-wide screening of DNA sequence copy number changes. RESULTS. Copy-number aberrations were detected in 20 (74%) tumors. The mean number of aberrations per tumor was 5.3 with gains outnumbering losses. The most frequent gains affected the minimal common regions of 7q11.2-q21 and 7q22 in 30% and 7p15-pter in 26%. Gain of 8q23-qter was detected in 22%. The most frequent... (More)
BACKGROUND. Radiotherapy is a known risk factor for sarcoma development. Postirradiation sarcomas arise within the radiation field after a latency period of several years and usually are highly malignant. Very little is yet known about their genetic changes. METHODS. Twenty-seven postirradiation sarcomas were analyzed by comparative genomic hybridization, which allows genome-wide screening of DNA sequence copy number changes. RESULTS. Copy-number aberrations were detected in 20 (74%) tumors. The mean number of aberrations per tumor was 5.3 with gains outnumbering losses. The most frequent gains affected the minimal common regions of 7q11.2-q21 and 7q22 in 30% and 7p15-pter in 26%. Gain of 8q23-qter was detected in 22%. The most frequent losses affected 11q23-qter and 13q22-q32 in 22%. In osteosarcomas, the most frequent aberration was loss of 1p21-p31, in malignant fibrous histiocytomas (MFH) gain of 7cen-q22, and in fibrosarcomas gain of 7q22. The findings in postirradiation osteosarcomas and MFHs were compared with findings in sporadic osteosarcomas and MFHs, reported previously by the authors. In sporadic osteosarcomas, gains outnumbered losses, but, in postirradiation osteosarcomas, losses were more frequent than gains. Loss at 1p was rare in sporadic osteosarcoma (3%) but frequent (57%) in postirradiation osteosarcomas. Gains at 7q were frequent both in postirradiation and sporadic MFH. CONCLUSIONS. According to previous studies on different types of sporadic sarcomas, gains at 7q or 8q are associated with poor prognosis or large tumor size. Thus, the frequent gains at 7q and 8q might have been responsible in part for the poor prognosis of postirradiation sarcomas. Also, however, some of their clinical features, i.e., high malignancy grade, late diagnosis, and central location, are associated with a poor prognosis. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
comparative genomic hybridization, postirradiation sarcoma, angiosarcoma, osteosarcoma, malignant fibrous histiocytoma (MFH), fibrosarcoma
in
Cancer
volume
92
issue
7
pages
1992 - 1998
publisher
John Wiley & Sons
external identifiers
  • wos:000171476400031
  • scopus:0035477483
ISSN
1097-0142
DOI
10.1002/1097-0142(20011001)92:7<1992::AID-CNCR1719>3.0.CO;2-2
language
English
LU publication?
yes
id
d264f650-70eb-49b8-8ce2-a3bff734032c (old id 1118831)
date added to LUP
2008-07-16 09:40:21
date last changed
2018-05-29 12:20:00
@article{d264f650-70eb-49b8-8ce2-a3bff734032c,
  abstract     = {BACKGROUND. Radiotherapy is a known risk factor for sarcoma development. Postirradiation sarcomas arise within the radiation field after a latency period of several years and usually are highly malignant. Very little is yet known about their genetic changes. METHODS. Twenty-seven postirradiation sarcomas were analyzed by comparative genomic hybridization, which allows genome-wide screening of DNA sequence copy number changes. RESULTS. Copy-number aberrations were detected in 20 (74%) tumors. The mean number of aberrations per tumor was 5.3 with gains outnumbering losses. The most frequent gains affected the minimal common regions of 7q11.2-q21 and 7q22 in 30% and 7p15-pter in 26%. Gain of 8q23-qter was detected in 22%. The most frequent losses affected 11q23-qter and 13q22-q32 in 22%. In osteosarcomas, the most frequent aberration was loss of 1p21-p31, in malignant fibrous histiocytomas (MFH) gain of 7cen-q22, and in fibrosarcomas gain of 7q22. The findings in postirradiation osteosarcomas and MFHs were compared with findings in sporadic osteosarcomas and MFHs, reported previously by the authors. In sporadic osteosarcomas, gains outnumbered losses, but, in postirradiation osteosarcomas, losses were more frequent than gains. Loss at 1p was rare in sporadic osteosarcoma (3%) but frequent (57%) in postirradiation osteosarcomas. Gains at 7q were frequent both in postirradiation and sporadic MFH. CONCLUSIONS. According to previous studies on different types of sporadic sarcomas, gains at 7q or 8q are associated with poor prognosis or large tumor size. Thus, the frequent gains at 7q and 8q might have been responsible in part for the poor prognosis of postirradiation sarcomas. Also, however, some of their clinical features, i.e., high malignancy grade, late diagnosis, and central location, are associated with a poor prognosis.},
  author       = {Tarkkanen, Maija and Wiklund, Tom A. and Virolainen, Martti J. and Larramendy, Marcelo L. and Mandahl, Nils and Mertens, Fredrik and Blomqvist, Carl P. and Tukiainen, Erkki J. and Miettinen, Markku M. A. and Elomaa, A. Inkeri and Knuutila, Y. Sakari},
  issn         = {1097-0142},
  keyword      = {comparative genomic hybridization,postirradiation sarcoma,angiosarcoma,osteosarcoma,malignant fibrous histiocytoma (MFH),fibrosarcoma},
  language     = {eng},
  number       = {7},
  pages        = {1992--1998},
  publisher    = {John Wiley & Sons},
  series       = {Cancer},
  title        = {Comparative genomic hybridization of postirradiation sarcomas},
  url          = {http://dx.doi.org/10.1002/1097-0142(20011001)92:7<1992::AID-CNCR1719>3.0.CO;2-2},
  volume       = {92},
  year         = {2001},
}