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Migration of cells into and out of peripheral nerve isografts in the peripheral and central nervous systems of the adult mouse

Symons, Natalie A.; Danielsen, Nils LU and Harvey, Alan R. (2001) In European Journal of Neuroscience 14(3). p.522-532
Abstract
Peripheral nerve (PN) isografts provide a favourable environment for axon regeneration after peripheral and central nervous system (CNS) injury, but definitive information on the extent of cellular intermixing between donor and host tissues is lacking. We wished to compare migration patterns in fresh and predegenerate PN grafts, and also compare the extent of cell migration after transplantation to peripheral nervous system (PNS) versus CNS. To discern how host and donor cells interact after PIN transplantation, sciatic nerve segments were transplanted from inbred adult mice into PN defects (PN-PN grafts) or into lesioned cerebral cortex of opposite gender siblings. Migrating male cells were identified using a Y-chromosome-specific probe... (More)
Peripheral nerve (PN) isografts provide a favourable environment for axon regeneration after peripheral and central nervous system (CNS) injury, but definitive information on the extent of cellular intermixing between donor and host tissues is lacking. We wished to compare migration patterns in fresh and predegenerate PN grafts, and also compare the extent of cell migration after transplantation to peripheral nervous system (PNS) versus CNS. To discern how host and donor cells interact after PIN transplantation, sciatic nerve segments were transplanted from inbred adult mice into PN defects (PN-PN grafts) or into lesioned cerebral cortex of opposite gender siblings. Migrating male cells were identified using a Y-chromosome-specific probe and in situ hybridization methods, and characterized immunohistochemically. The extent of donor and host cellular intermixing was similar in fresh and predegenerate PN-PN isografts. There was substantial intermixing of donor and host cells by 8 days. Many host cells migrating into epineurial regions of grafts were immunopositive for F4/80 (macrophages). The endoneurium of grafted PN was also colonized by host cells; some were F4/80(+) but many were immunostained with S-100 (Schwann cell marker). Donor S-100(+) Schwann cells rapidly migrated out into proximal and distal host PN and by 12 weeks were found at least 2 mm from the grafts. Endoneurial microvessels in grafts were mostly donor-derived. By comparison, in male PN grafts to female CNS, even after 6 weeks few donor cells had migrated out into surrounding host cortex, despite the observation that almost all grafts contained regenerating axons and were thus attached to host CNS tissue. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
axon regeneration, macrophages, revascularization, Schwann cells, transplantation
in
European Journal of Neuroscience
volume
14
issue
3
pages
522 - 532
publisher
Wiley-Blackwell
external identifiers
  • wos:000171184400012
  • scopus:0035724850
ISSN
1460-9568
DOI
language
English
LU publication?
yes
id
3228c6d9-4326-4174-ac3c-6b5cd83a601a (old id 1118876)
date added to LUP
2008-07-16 09:34:25
date last changed
2018-05-29 11:22:34
@article{3228c6d9-4326-4174-ac3c-6b5cd83a601a,
  abstract     = {Peripheral nerve (PN) isografts provide a favourable environment for axon regeneration after peripheral and central nervous system (CNS) injury, but definitive information on the extent of cellular intermixing between donor and host tissues is lacking. We wished to compare migration patterns in fresh and predegenerate PN grafts, and also compare the extent of cell migration after transplantation to peripheral nervous system (PNS) versus CNS. To discern how host and donor cells interact after PIN transplantation, sciatic nerve segments were transplanted from inbred adult mice into PN defects (PN-PN grafts) or into lesioned cerebral cortex of opposite gender siblings. Migrating male cells were identified using a Y-chromosome-specific probe and in situ hybridization methods, and characterized immunohistochemically. The extent of donor and host cellular intermixing was similar in fresh and predegenerate PN-PN isografts. There was substantial intermixing of donor and host cells by 8 days. Many host cells migrating into epineurial regions of grafts were immunopositive for F4/80 (macrophages). The endoneurium of grafted PN was also colonized by host cells; some were F4/80(+) but many were immunostained with S-100 (Schwann cell marker). Donor S-100(+) Schwann cells rapidly migrated out into proximal and distal host PN and by 12 weeks were found at least 2 mm from the grafts. Endoneurial microvessels in grafts were mostly donor-derived. By comparison, in male PN grafts to female CNS, even after 6 weeks few donor cells had migrated out into surrounding host cortex, despite the observation that almost all grafts contained regenerating axons and were thus attached to host CNS tissue.},
  author       = {Symons, Natalie A. and Danielsen, Nils and Harvey, Alan R.},
  issn         = {1460-9568},
  keyword      = {axon regeneration,macrophages,revascularization,Schwann cells,transplantation},
  language     = {eng},
  number       = {3},
  pages        = {522--532},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Neuroscience},
  title        = {Migration of cells into and out of peripheral nerve isografts in the peripheral and central nervous systems of the adult mouse},
  url          = {http://dx.doi.org/},
  volume       = {14},
  year         = {2001},
}