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A role for hormone-sensitive lipase in the selective mobilization of adipose tissue fatty acids

Raclot, Thierry; Holm, Cecilia LU and Langin, Dominique (2001) In Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1532(1-2). p.88-96
Abstract
The mobilization of fatty acids from rat and human fat cells is selective according to molecular structure, and notably carbon atom chain length. This study aimed at examining whether the release of individual fatty acids from triacylglycerols (TAG) by hormone-sensitive lipase (HSL) plays a role in the selectivity of fatty acid mobilization. Recombinant rat and human HSL were incubated with a lipid emulsion. The hydrolysis of 18 individual fatty acids, ranging in chain length from 12 to 24 carbon atoms and in unsaturation degree from 0 to 3 double bond(s), was measured by comparing the composition of non-esterified fatty acids (NEFA) to that of the original TAG. The relative hydrolysis (% in NEFA/% in TAG) differed between fatty acids,... (More)
The mobilization of fatty acids from rat and human fat cells is selective according to molecular structure, and notably carbon atom chain length. This study aimed at examining whether the release of individual fatty acids from triacylglycerols (TAG) by hormone-sensitive lipase (HSL) plays a role in the selectivity of fatty acid mobilization. Recombinant rat and human HSL were incubated with a lipid emulsion. The hydrolysis of 18 individual fatty acids, ranging in chain length from 12 to 24 carbon atoms and in unsaturation degree from 0 to 3 double bond(s), was measured by comparing the composition of non-esterified fatty acids (NEFA) to that of the original TAG. The relative hydrolysis (% in NEFA/% in TAG) differed between fatty acids, being about 5-fold and 3-fold higher for the most(18:1n-7) than for the least (24:0) readily released fatty acid by recombinant rat and human HSL, respectively. Relationships were found between the chain length of fatty acids and their relative hydrolysis. Among 12-24 carbon atom saturated fatty acids, the relative hydrolysis markedly decreased (by about 5- and 3-times for recombinant rat and human HSL, respectively) with increasing chain length. We conclude that fatty acids are selectively released from TAG by HSL according to carbon atom chain length. These data provide insight on the mechanism by which fatty acids are selectively mobilized from fat cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adipose tissue, Triacylglycerol, Lipolysis, Fatty acid molecular structure, Lipid emulsion
in
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
volume
1532
issue
1-2
pages
88 - 96
publisher
Elsevier
external identifiers
  • wos:000169590100009
  • scopus:0035978432
ISSN
1388-1981
DOI
language
English
LU publication?
yes
id
4219dafe-a5aa-4819-bc29-32ef169acb44 (old id 1119096)
date added to LUP
2008-07-10 10:38:43
date last changed
2018-05-29 09:40:19
@article{4219dafe-a5aa-4819-bc29-32ef169acb44,
  abstract     = {The mobilization of fatty acids from rat and human fat cells is selective according to molecular structure, and notably carbon atom chain length. This study aimed at examining whether the release of individual fatty acids from triacylglycerols (TAG) by hormone-sensitive lipase (HSL) plays a role in the selectivity of fatty acid mobilization. Recombinant rat and human HSL were incubated with a lipid emulsion. The hydrolysis of 18 individual fatty acids, ranging in chain length from 12 to 24 carbon atoms and in unsaturation degree from 0 to 3 double bond(s), was measured by comparing the composition of non-esterified fatty acids (NEFA) to that of the original TAG. The relative hydrolysis (% in NEFA/% in TAG) differed between fatty acids, being about 5-fold and 3-fold higher for the most(18:1n-7) than for the least (24:0) readily released fatty acid by recombinant rat and human HSL, respectively. Relationships were found between the chain length of fatty acids and their relative hydrolysis. Among 12-24 carbon atom saturated fatty acids, the relative hydrolysis markedly decreased (by about 5- and 3-times for recombinant rat and human HSL, respectively) with increasing chain length. We conclude that fatty acids are selectively released from TAG by HSL according to carbon atom chain length. These data provide insight on the mechanism by which fatty acids are selectively mobilized from fat cells.},
  author       = {Raclot, Thierry and Holm, Cecilia and Langin, Dominique},
  issn         = {1388-1981},
  keyword      = {Adipose tissue,Triacylglycerol,Lipolysis,Fatty acid molecular structure,Lipid emulsion},
  language     = {eng},
  number       = {1-2},
  pages        = {88--96},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids},
  title        = {A role for hormone-sensitive lipase in the selective mobilization of adipose tissue fatty acids},
  url          = {http://dx.doi.org/},
  volume       = {1532},
  year         = {2001},
}