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Characterization of chromosomal abnormalities in uroepithelial carcinomas by G-banding, spectral karyotyping and fish analysis

Fadl-Elmula, Imad ; Kytola, Soili ; Pan, Yi ; Lui, Weng-Onn ; Derienzo, Gaetano ; Forsberg, Lars ; Mandahl, Nils LU ; Gorunova, Ludmila LU ; Bergerheim, Ulf S.R. and Heim, Sverre , et al. (2001) In International Journal of Cancer 92(6). p.824-831
Abstract
Chromosome analysis by G-banding, spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) was per formed on 24 short-term cultured transitional cell bladder carcinomas and 5 cell lines established from bladder carcinomas. Except for one tumor with an apparently normal chromosomal constitution, clonal chromosome abnormalities were detected in all examined cases by the combined approach. The application of SKY and FISH techniques improved the karyotypic descriptions, originally based on C-banding only, by identifying 32 additional numerical changes, by establishing the chromosomal origin of 27 markers and 2 ring chromosomes, by redefining 53 aberrations and by detecting 15 hidden chromosomal rearrangements. No recurrent... (More)
Chromosome analysis by G-banding, spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) was per formed on 24 short-term cultured transitional cell bladder carcinomas and 5 cell lines established from bladder carcinomas. Except for one tumor with an apparently normal chromosomal constitution, clonal chromosome abnormalities were detected in all examined cases by the combined approach. The application of SKY and FISH techniques improved the karyotypic descriptions, originally based on C-banding only, by identifying 32 additional numerical changes, by establishing the chromosomal origin of 27 markers and 2 ring chromosomes, by redefining 53 aberrations and by detecting 15 hidden chromosomal rearrangements. No recurrent translocation, however, was detected. The most prominent: karyotypic feature was thus the occurrence of deletions and losses of whole chromosome copies indicating the importance of tumor suppressor genes in transitional cell carcinoma pathogenesis. Invasive carcinomas were karyotypically more complex than were low grade superficial tumors. Specific leases of material from chromosome 9 and from chromosome arms I Ip and 8p, and gains of 8q and Iq seem to be early changes appearing in superficial tumors, whereas losses from 4p and 17p and the formation of an isochromosome for 5p were associated with more aggressive tumor phenotypes. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bladder cancer, SKY, FISH, G-banding, cancer cytogenetics, chromosomal abnormalities
in
International Journal of Cancer
volume
92
issue
6
pages
824 - 831
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000168834000008
  • scopus:0035876969
  • pmid:11351302
ISSN
0020-7136
DOI
10.1002/ijc.1267
language
English
LU publication?
yes
id
b90b44f9-6c40-4b6d-8e5b-01412a04885d (old id 1119186)
date added to LUP
2016-04-01 11:50:49
date last changed
2025-04-04 14:28:41
@article{b90b44f9-6c40-4b6d-8e5b-01412a04885d,
  abstract     = {{Chromosome analysis by G-banding, spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) was per formed on 24 short-term cultured transitional cell bladder carcinomas and 5 cell lines established from bladder carcinomas. Except for one tumor with an apparently normal chromosomal constitution, clonal chromosome abnormalities were detected in all examined cases by the combined approach. The application of SKY and FISH techniques improved the karyotypic descriptions, originally based on C-banding only, by identifying 32 additional numerical changes, by establishing the chromosomal origin of 27 markers and 2 ring chromosomes, by redefining 53 aberrations and by detecting 15 hidden chromosomal rearrangements. No recurrent translocation, however, was detected. The most prominent: karyotypic feature was thus the occurrence of deletions and losses of whole chromosome copies indicating the importance of tumor suppressor genes in transitional cell carcinoma pathogenesis. Invasive carcinomas were karyotypically more complex than were low grade superficial tumors. Specific leases of material from chromosome 9 and from chromosome arms I Ip and 8p, and gains of 8q and Iq seem to be early changes appearing in superficial tumors, whereas losses from 4p and 17p and the formation of an isochromosome for 5p were associated with more aggressive tumor phenotypes.}},
  author       = {{Fadl-Elmula, Imad and Kytola, Soili and Pan, Yi and Lui, Weng-Onn and Derienzo, Gaetano and Forsberg, Lars and Mandahl, Nils and Gorunova, Ludmila and Bergerheim, Ulf S.R. and Heim, Sverre and Larsson, Catharina}},
  issn         = {{0020-7136}},
  keywords     = {{bladder cancer; SKY; FISH; G-banding; cancer cytogenetics; chromosomal abnormalities}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{824--831}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{International Journal of Cancer}},
  title        = {{Characterization of chromosomal abnormalities in uroepithelial carcinomas by G-banding, spectral karyotyping and fish analysis}},
  url          = {{http://dx.doi.org/10.1002/ijc.1267}},
  doi          = {{10.1002/ijc.1267}},
  volume       = {{92}},
  year         = {{2001}},
}