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Proteoglycans and hyaluronan in human fetal membranes

Meinert, Mette; Eriksen, Gitte V.; Petersen, Astrid C.; Helmig, Rikke B.; Laurent, Claude; Uldbjerg, Niels and Malmström, Anders LU (2001) In American Journal of Obstetrics and Gynecology 184(4). p.679-685
Abstract
OBJECTIVE: The aim of this study was to describe the distributions of major extracellular matrix components, such as proteoglycans, collagen acid hyaluronan, in the fetal membranes at term. STUDY DESIGN: Fetal membranes were obtained from elective cesarean deliveries at term. Guanidinium extracts were analyzed for proteoglycans with alcian blue precipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western blotting and for hyaluronan with a radioimmunoassay. Collagen was measured by estimating hydroxyproline content. Tissue sections were immunostained for decorin and biglycan and stained for hyaluronan with a biotin-labeled hyaluronan-binding protein. RESULTS: The fetal membranes contained predominantly smaller... (More)
OBJECTIVE: The aim of this study was to describe the distributions of major extracellular matrix components, such as proteoglycans, collagen acid hyaluronan, in the fetal membranes at term. STUDY DESIGN: Fetal membranes were obtained from elective cesarean deliveries at term. Guanidinium extracts were analyzed for proteoglycans with alcian blue precipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western blotting and for hyaluronan with a radioimmunoassay. Collagen was measured by estimating hydroxyproline content. Tissue sections were immunostained for decorin and biglycan and stained for hyaluronan with a biotin-labeled hyaluronan-binding protein. RESULTS: The fetal membranes contained predominantly smaller proteoglycans, such as biglycan and decorin. The amnion consisted of typical fibrous connective tissue with a high concentration of collagen. The amnion was dominated by decorin located in close connection with the collagen fibrils. The chorion was composed of a fibroblastic part containing collagen and decorin and a trophoblastic part mainly containing biglycan. In addition, large amounts of hyaluronan were found, especially in the amnion and in the decidual cell layers. CONCLUSION: The distributions of proteoglycans, collagen, and hyaluronan in human fetal membranes may explain the biomechanical properties of this tissue. We suggest that changes in the relative proportions of these extracellular molecules are crucial for the proposed maturation process in the fetal membranes during the last weeks of pregnancy. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of Obstetrics and Gynecology
volume
184
issue
4
pages
679 - 685
publisher
Elsevier
external identifiers
  • wos:000167841300026
  • scopus:0035077381
ISSN
1097-6868
DOI
10.1067/mob.2001.110294
language
English
LU publication?
yes
id
97d5c59e-dd8f-480a-be26-d6f6102ae53a (old id 1119358)
date added to LUP
2008-07-04 15:57:39
date last changed
2018-06-03 03:42:59
@article{97d5c59e-dd8f-480a-be26-d6f6102ae53a,
  abstract     = {OBJECTIVE: The aim of this study was to describe the distributions of major extracellular matrix components, such as proteoglycans, collagen acid hyaluronan, in the fetal membranes at term. STUDY DESIGN: Fetal membranes were obtained from elective cesarean deliveries at term. Guanidinium extracts were analyzed for proteoglycans with alcian blue precipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western blotting and for hyaluronan with a radioimmunoassay. Collagen was measured by estimating hydroxyproline content. Tissue sections were immunostained for decorin and biglycan and stained for hyaluronan with a biotin-labeled hyaluronan-binding protein. RESULTS: The fetal membranes contained predominantly smaller proteoglycans, such as biglycan and decorin. The amnion consisted of typical fibrous connective tissue with a high concentration of collagen. The amnion was dominated by decorin located in close connection with the collagen fibrils. The chorion was composed of a fibroblastic part containing collagen and decorin and a trophoblastic part mainly containing biglycan. In addition, large amounts of hyaluronan were found, especially in the amnion and in the decidual cell layers. CONCLUSION: The distributions of proteoglycans, collagen, and hyaluronan in human fetal membranes may explain the biomechanical properties of this tissue. We suggest that changes in the relative proportions of these extracellular molecules are crucial for the proposed maturation process in the fetal membranes during the last weeks of pregnancy.},
  author       = {Meinert, Mette and Eriksen, Gitte V. and Petersen, Astrid C. and Helmig, Rikke B. and Laurent, Claude and Uldbjerg, Niels and Malmström, Anders},
  issn         = {1097-6868},
  language     = {eng},
  number       = {4},
  pages        = {679--685},
  publisher    = {Elsevier},
  series       = {American Journal of Obstetrics and Gynecology},
  title        = {Proteoglycans and hyaluronan in human fetal membranes},
  url          = {http://dx.doi.org/10.1067/mob.2001.110294},
  volume       = {184},
  year         = {2001},
}