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Differential usage of IκBα and IκBβ in regulation of apoptosis versus gene expression

Lindgren, Hanna; Olsson, Anders; Pero, Ronald LU and Leanderson, Tomas LU (2003) In Biochemical and Biophysical Research Communications 301(1). p.204-211
Abstract
n this study we use the N-substituted benzamides declopramide (3-CPA) and N-acetyl declopramide (Na-3-CPA) to investigate the involvement of the transcription factor NF-κB in the induction of apoptosis and surface immunoglobulin κ (Igκ) expression in the mouse pre-B cell line 70Z/3. We first showed that 3-CPA-induced apoptosis at doses around 500 μM and that the 3-CPA-induced apoptosis could be suppressed by over-expression of the Bcl-2 protein. Na-3-CPA was shown to be non-apoptotic at doses up to 1–2 mM. On the other hand, Na-3-CPA inhibited LPS-induced Igκ expression while 3-CPA had no effect. Further analysis showed that while 3-CPA inhibited breakdown of IκBα, Na-3-CPA inhibited breakdown of IκBβ. In addition, we used a 70Z/3 cell... (More)
n this study we use the N-substituted benzamides declopramide (3-CPA) and N-acetyl declopramide (Na-3-CPA) to investigate the involvement of the transcription factor NF-κB in the induction of apoptosis and surface immunoglobulin κ (Igκ) expression in the mouse pre-B cell line 70Z/3. We first showed that 3-CPA-induced apoptosis at doses around 500 μM and that the 3-CPA-induced apoptosis could be suppressed by over-expression of the Bcl-2 protein. Na-3-CPA was shown to be non-apoptotic at doses up to 1–2 mM. On the other hand, Na-3-CPA inhibited LPS-induced Igκ expression while 3-CPA had no effect. Further analysis showed that while 3-CPA inhibited breakdown of IκBα, Na-3-CPA inhibited breakdown of IκBβ. In addition, we used a 70Z/3 cell line expressing a dominant negative IκBα (70Z/3ΔNIκBα). The 70Z/3ΔNIκBα cell line was shown to be more sensitive to apoptosis and cytotoxicity induced by 3-CPA as well as by LPS, probably due to a defect in NF-κB rescue mechanism. Taken together, our data implicate distinct roles for IκBα and IκBβ in regulating various NF-κB activities. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
NF-κB, Apoptosis, IκB, N-substituted benzamides
in
Biochemical and Biophysical Research Communications
volume
301
issue
1
pages
204 - 211
publisher
Elsevier
external identifiers
  • pmid:12535663
  • wos:000181052800033
  • scopus:0037473754
ISSN
1090-2104
DOI
10.1016/S0006-291X(02)03012-7
language
English
LU publication?
yes
id
6bee2753-f53b-4a11-b6b7-ba6e7490503a (old id 111940)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12535663&dopt=Abstract
date added to LUP
2007-06-26 10:47:20
date last changed
2018-05-29 10:16:42
@article{6bee2753-f53b-4a11-b6b7-ba6e7490503a,
  abstract     = {n this study we use the N-substituted benzamides declopramide (3-CPA) and N-acetyl declopramide (Na-3-CPA) to investigate the involvement of the transcription factor NF-κB in the induction of apoptosis and surface immunoglobulin κ (Igκ) expression in the mouse pre-B cell line 70Z/3. We first showed that 3-CPA-induced apoptosis at doses around 500 μM and that the 3-CPA-induced apoptosis could be suppressed by over-expression of the Bcl-2 protein. Na-3-CPA was shown to be non-apoptotic at doses up to 1–2 mM. On the other hand, Na-3-CPA inhibited LPS-induced Igκ expression while 3-CPA had no effect. Further analysis showed that while 3-CPA inhibited breakdown of IκBα, Na-3-CPA inhibited breakdown of IκBβ. In addition, we used a 70Z/3 cell line expressing a dominant negative IκBα (70Z/3ΔNIκBα). The 70Z/3ΔNIκBα cell line was shown to be more sensitive to apoptosis and cytotoxicity induced by 3-CPA as well as by LPS, probably due to a defect in NF-κB rescue mechanism. Taken together, our data implicate distinct roles for IκBα and IκBβ in regulating various NF-κB activities.},
  author       = {Lindgren, Hanna and Olsson, Anders and Pero, Ronald and Leanderson, Tomas},
  issn         = {1090-2104},
  keyword      = {NF-κB,Apoptosis,IκB,N-substituted benzamides},
  language     = {eng},
  number       = {1},
  pages        = {204--211},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Differential usage of IκBα and IκBβ in regulation of apoptosis versus gene expression},
  url          = {http://dx.doi.org/10.1016/S0006-291X(02)03012-7},
  volume       = {301},
  year         = {2003},
}