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A PKCbeta isoform mediates phorbol ester-induced activation of Erk1/2 and expression of neuronal differentiation genes in neuroblastoma cells

Trollér, Ulrika LU ; Zeidman, Ruth LU ; Svensson, Karin and Larsson, Christer LU (2001) In FEBS Letters 508(1). p.126-130
Abstract
Protein kinase C (PKC) activation induces neuronal differentiation of SH-SY5Y neuroblastoma cells. This study examines the role of PKCbeta isoforms in this process. The PKCbeta-specific inhibitor LY379196 had no effect on 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced neurite outgrowth from SH-SY5Y neuroblastoma cells. On the other hand, PKCbeta inhibition suppressed the TPA-stimulated increase in neuropeptide Y mRNA, activation of neuropeptide Y gene promoter elements, and phosphorylation of Erk1/2. The TPA-induced increase in neuropeptide Y expression was also inhibited by the MEK inhibitor PD98059. These data indicate that activation of a PKCbeta isoform, through a pathway involving Erk1/2, leads to increased expression of neuronal... (More)
Protein kinase C (PKC) activation induces neuronal differentiation of SH-SY5Y neuroblastoma cells. This study examines the role of PKCbeta isoforms in this process. The PKCbeta-specific inhibitor LY379196 had no effect on 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced neurite outgrowth from SH-SY5Y neuroblastoma cells. On the other hand, PKCbeta inhibition suppressed the TPA-stimulated increase in neuropeptide Y mRNA, activation of neuropeptide Y gene promoter elements, and phosphorylation of Erk1/2. The TPA-induced increase in neuropeptide Y expression was also inhibited by the MEK inhibitor PD98059. These data indicate that activation of a PKCbeta isoform, through a pathway involving Erk1/2, leads to increased expression of neuronal differentiation genes in neuroblastoma cells. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
keywords
Growth-associated protein 43, Mitogen-activated protein kinase, Neuroblastoma cell, Neuronal differentiation, Neuropeptide Y, Protein kinase C
in
FEBS Letters
volume
508
issue
1
pages
126 - 130
publisher
Wiley-Blackwell
external identifiers
  • pmid:11707282
  • scopus:0035834240
ISSN
1873-3468
DOI
10.1016/S0014-5793(01)03043-5
language
English
LU publication?
yes
id
f7d7255a-be38-4748-a8ed-3051a1441985 (old id 1119765)
date added to LUP
2008-07-16 11:22:26
date last changed
2018-05-29 10:41:38
@article{f7d7255a-be38-4748-a8ed-3051a1441985,
  abstract     = {Protein kinase C (PKC) activation induces neuronal differentiation of SH-SY5Y neuroblastoma cells. This study examines the role of PKCbeta isoforms in this process. The PKCbeta-specific inhibitor LY379196 had no effect on 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced neurite outgrowth from SH-SY5Y neuroblastoma cells. On the other hand, PKCbeta inhibition suppressed the TPA-stimulated increase in neuropeptide Y mRNA, activation of neuropeptide Y gene promoter elements, and phosphorylation of Erk1/2. The TPA-induced increase in neuropeptide Y expression was also inhibited by the MEK inhibitor PD98059. These data indicate that activation of a PKCbeta isoform, through a pathway involving Erk1/2, leads to increased expression of neuronal differentiation genes in neuroblastoma cells.},
  author       = {Trollér, Ulrika and Zeidman, Ruth and Svensson, Karin and Larsson, Christer},
  issn         = {1873-3468},
  keyword      = {Growth-associated protein 43,Mitogen-activated protein kinase,Neuroblastoma cell,Neuronal differentiation,Neuropeptide Y,Protein kinase C},
  language     = {eng},
  number       = {1},
  pages        = {126--130},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {A PKCbeta isoform mediates phorbol ester-induced activation of Erk1/2 and expression of neuronal differentiation genes in neuroblastoma cells},
  url          = {http://dx.doi.org/10.1016/S0014-5793(01)03043-5},
  volume       = {508},
  year         = {2001},
}