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Reduced postnatal cerebral glucose metabolism measured by PET after asphyxia in near term fetal lambs

Thorngren-Jerneck, Kristina LU ; Ley, David LU ; Hellström-Westas, Lena LU ; Hernandez-Andrade, Edgar LU ; Lingman, Göran LU ; Ohlsson, Tomas G LU ; Oskarsson, Gylfi; Pesonen, Erkki LU ; Sandell, Anders LU and Strand, Sven-Erik LU , et al. (2001) In Journal of Neuroscience Research 66(5). p.844-850
Abstract
The effects of fetal asphyxia on cerebral function and development, involve the transition from fetal to neonatal life. Changes in cerebral glucose metabolism may be an early postnatal indicator of fetal asphyxia. The objective is to develop an experimental lamb model involving the transition from fetal to neonatal life and to examine the effect of fetal asphyxia with cerebral hypoxic ischemia on early postnatal cerebral glucose metabolism. Fetal asphyxia was induced by total umbilical cord occlusion in eight near-term fetal lambs (134-138 days) with the ewe under isoflurane-opiate anesthesia. The mean occlusion time until cardiac arrest was 14.5 (4.2) min (SD). Lambs were immediately delivered and standardized resuscitation was instituted... (More)
The effects of fetal asphyxia on cerebral function and development, involve the transition from fetal to neonatal life. Changes in cerebral glucose metabolism may be an early postnatal indicator of fetal asphyxia. The objective is to develop an experimental lamb model involving the transition from fetal to neonatal life and to examine the effect of fetal asphyxia with cerebral hypoxic ischemia on early postnatal cerebral glucose metabolism. Fetal asphyxia was induced by total umbilical cord occlusion in eight near-term fetal lambs (134-138 days) with the ewe under isoflurane-opiate anesthesia. The mean occlusion time until cardiac arrest was 14.5 (4.2) min (SD). Lambs were immediately delivered and standardized resuscitation was instituted after 2 min asystole. At 4 hr postnatal age, [18-F]Fluoro-2-deoxy-glucose (18-FDG) was injected intravenously in eight asphyxiated lambs and in eight controls. Cerebral glucose metabolism was examined by positron emission tomography (PET). As a result the mean arterial blood pressure, acid-base values, blood glucose and serum lactate at 4 hr postnatal age did not differ significantly between lambs subjected to umbilical cord occlusion and controls. EEG was abnormal in all lambs subjected to cord occlusion and normal in the controls at 4 hr postnatal age. Global cerebral metabolic rate (CMRgl) as determined by PET was significantly lower in lambs subjected to cord occlusion mean/median (SD) 22.2/19.6 (8.4) micromol/min/100 g) than in controls mean/median (SD) 37.8/35.9 (6.1); P < 0.01). Global CMRgl is significantly reduced in newborn lambs 4 hr after fetal asphyxia induced by umbilical cord occlusion. A reduction in CMRgl is an early indicator of global hypoxic cerebral ischemia. (Less)
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publication status
published
subject
keywords
fetal lamb, asphyxia, cerebral glucose metabolism, positron emission tomography
in
Journal of Neuroscience Research
volume
66
issue
5
pages
844 - 850
publisher
John Wiley & Sons
external identifiers
  • pmid:11746410
  • scopus:0035577285
ISSN
1097-4547
DOI
10.1002/jnr.10051
language
English
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yes
id
fbfbfb28-18bc-49a7-816f-aaeb85478920 (old id 1119931)
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2008-07-16 10:27:05
date last changed
2018-01-07 08:53:01
@article{fbfbfb28-18bc-49a7-816f-aaeb85478920,
  abstract     = {The effects of fetal asphyxia on cerebral function and development, involve the transition from fetal to neonatal life. Changes in cerebral glucose metabolism may be an early postnatal indicator of fetal asphyxia. The objective is to develop an experimental lamb model involving the transition from fetal to neonatal life and to examine the effect of fetal asphyxia with cerebral hypoxic ischemia on early postnatal cerebral glucose metabolism. Fetal asphyxia was induced by total umbilical cord occlusion in eight near-term fetal lambs (134-138 days) with the ewe under isoflurane-opiate anesthesia. The mean occlusion time until cardiac arrest was 14.5 (4.2) min (SD). Lambs were immediately delivered and standardized resuscitation was instituted after 2 min asystole. At 4 hr postnatal age, [18-F]Fluoro-2-deoxy-glucose (18-FDG) was injected intravenously in eight asphyxiated lambs and in eight controls. Cerebral glucose metabolism was examined by positron emission tomography (PET). As a result the mean arterial blood pressure, acid-base values, blood glucose and serum lactate at 4 hr postnatal age did not differ significantly between lambs subjected to umbilical cord occlusion and controls. EEG was abnormal in all lambs subjected to cord occlusion and normal in the controls at 4 hr postnatal age. Global cerebral metabolic rate (CMRgl) as determined by PET was significantly lower in lambs subjected to cord occlusion mean/median (SD) 22.2/19.6 (8.4) micromol/min/100 g) than in controls mean/median (SD) 37.8/35.9 (6.1); P &lt; 0.01). Global CMRgl is significantly reduced in newborn lambs 4 hr after fetal asphyxia induced by umbilical cord occlusion. A reduction in CMRgl is an early indicator of global hypoxic cerebral ischemia.},
  author       = {Thorngren-Jerneck, Kristina and Ley, David and Hellström-Westas, Lena and Hernandez-Andrade, Edgar and Lingman, Göran and Ohlsson, Tomas G and Oskarsson, Gylfi and Pesonen, Erkki and Sandell, Anders and Strand, Sven-Erik and Werner, Olof and Marsal, Karel},
  issn         = {1097-4547},
  keyword      = {fetal lamb,asphyxia,cerebral glucose metabolism,positron emission tomography},
  language     = {eng},
  number       = {5},
  pages        = {844--850},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Neuroscience Research},
  title        = {Reduced postnatal cerebral glucose metabolism measured by PET after asphyxia in near term fetal lambs},
  url          = {http://dx.doi.org/10.1002/jnr.10051},
  volume       = {66},
  year         = {2001},
}