Upregulation of Flt3 expression within the bone marrow Lin(-)Sca1(+)c-kit(+) stem cell compartment is accompanied by loss of self-renewal capacity
(2001) In Immunity 15(4). p.659-669- Abstract
- Flt3 has emerged as a potential regulator of hematopoietic stem cells (HSC). Sixty percent of cells in the mouse marrow Lin(-)Sca1(+)c-kit(+) HSC pool expressed flt3. Although single cell cloning showed comparable high proliferative, myeloid, B, and T cell potentials of Lin(-)Sca1(+)c-kit(+)flt3(+) and Lin(-)Sca1(+)c-kit(+)flt3(-) cells, only Lin(-)Sca1(+)c-kit(+)flt3(-) cells supported sustained multilineage reconstitution. In striking contrast, Lin(-)Sca1(+)c-kit(+)flt3(+) cells rapidly and efficiently reconstituted B and T lymphopoiesis, whereas myeloid reconstitution was exclusively short term. Unlike c-kit, activation of flt3 failed to support survival of HSC, whereas only flt3 mediated survival of Lin(-)Sca1(+)c-kit(+)flt3(+)... (More)
- Flt3 has emerged as a potential regulator of hematopoietic stem cells (HSC). Sixty percent of cells in the mouse marrow Lin(-)Sca1(+)c-kit(+) HSC pool expressed flt3. Although single cell cloning showed comparable high proliferative, myeloid, B, and T cell potentials of Lin(-)Sca1(+)c-kit(+)flt3(+) and Lin(-)Sca1(+)c-kit(+)flt3(-) cells, only Lin(-)Sca1(+)c-kit(+)flt3(-) cells supported sustained multilineage reconstitution. In striking contrast, Lin(-)Sca1(+)c-kit(+)flt3(+) cells rapidly and efficiently reconstituted B and T lymphopoiesis, whereas myeloid reconstitution was exclusively short term. Unlike c-kit, activation of flt3 failed to support survival of HSC, whereas only flt3 mediated survival of Lin(-)Sca1(+)c-kit(+)flt3(+) reconstituting cells. Phenotypic and functional analysis support that Lin(-)Sca1(+)c-kit(+)flt3(+) cells are progenitors for the common lymphoid progenitor. Thus, upregulation of flt3 expression on Lin(-)Sca1(+)c-kit(+) HSC cells is accompanied by loss of self-renewal capacity but sustained lymphoid-restricted reconstitution potential. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1119997
- author
- Adolfsson, Jörgen LU ; Borge, O J ; Bryder, David LU ; Theilgaard-Monch, K ; Åstrand-Grundström, Ingbritt LU ; Sitnicka Quinn, Ewa LU ; Sasaki, Yutaka LU and Jacobsen, Sten Eirik W LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Immunity
- volume
- 15
- issue
- 4
- pages
- 659 - 669
- publisher
- Cell Press
- external identifiers
-
- pmid:11672547
- scopus:0034749999
- ISSN
- 1074-7613
- DOI
- 10.1016/S1074-7613(01)00220-5
- language
- English
- LU publication?
- yes
- id
- 7bad8fbe-5572-4ef9-84d2-748f5600af5a (old id 1119997)
- date added to LUP
- 2016-04-01 11:48:08
- date last changed
- 2022-08-28 04:11:55
@article{7bad8fbe-5572-4ef9-84d2-748f5600af5a,
abstract = {{Flt3 has emerged as a potential regulator of hematopoietic stem cells (HSC). Sixty percent of cells in the mouse marrow Lin(-)Sca1(+)c-kit(+) HSC pool expressed flt3. Although single cell cloning showed comparable high proliferative, myeloid, B, and T cell potentials of Lin(-)Sca1(+)c-kit(+)flt3(+) and Lin(-)Sca1(+)c-kit(+)flt3(-) cells, only Lin(-)Sca1(+)c-kit(+)flt3(-) cells supported sustained multilineage reconstitution. In striking contrast, Lin(-)Sca1(+)c-kit(+)flt3(+) cells rapidly and efficiently reconstituted B and T lymphopoiesis, whereas myeloid reconstitution was exclusively short term. Unlike c-kit, activation of flt3 failed to support survival of HSC, whereas only flt3 mediated survival of Lin(-)Sca1(+)c-kit(+)flt3(+) reconstituting cells. Phenotypic and functional analysis support that Lin(-)Sca1(+)c-kit(+)flt3(+) cells are progenitors for the common lymphoid progenitor. Thus, upregulation of flt3 expression on Lin(-)Sca1(+)c-kit(+) HSC cells is accompanied by loss of self-renewal capacity but sustained lymphoid-restricted reconstitution potential.}},
author = {{Adolfsson, Jörgen and Borge, O J and Bryder, David and Theilgaard-Monch, K and Åstrand-Grundström, Ingbritt and Sitnicka Quinn, Ewa and Sasaki, Yutaka and Jacobsen, Sten Eirik W}},
issn = {{1074-7613}},
language = {{eng}},
number = {{4}},
pages = {{659--669}},
publisher = {{Cell Press}},
series = {{Immunity}},
title = {{Upregulation of Flt3 expression within the bone marrow Lin(-)Sca1(+)c-kit(+) stem cell compartment is accompanied by loss of self-renewal capacity}},
url = {{http://dx.doi.org/10.1016/S1074-7613(01)00220-5}},
doi = {{10.1016/S1074-7613(01)00220-5}},
volume = {{15}},
year = {{2001}},
}