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Analysis of the distribution and frequency of trisomy 7 in vivo in synovia from patients with osteoarthritis and pigmented villonodular synovitis

Dahlén, A LU ; Broberg, K LU ; Domanski, H A LU ; Toksvig-Larsen, S LU ; Lindstrand, A LU ; Mandahl, N LU and Mertens, F LU (2001) In Cancer Genetics and Cytogenetics 131(1). p.19-24
Abstract

Osteoarthritis (OA) and pigmented villonodular synovitis (PVNS) are disorders associated with trisomy 7. The aim of the present study was to determine the frequency and distribution of the cells with +7 in vivo by analyzing sections of paraffin-embedded synovia from patients affected by OA, PVNS, other forms of synovitis [hemorragic synovitis (HS) and chronic synovitis (CS)], and from individuals without joint disease. Fluorescence in situ hybridization (FISH), using a centromeric probe for chromosome 7, showed that the mean frequency of trisomic nuclei in 5-microm sections was highest in PVNS (9.0%), followed by CS (5.9%), OA (5.6%), and HS (4.6%), whereas trisomic nuclei were rare (0.7%) in normal tissue. When 8-microm sections were... (More)

Osteoarthritis (OA) and pigmented villonodular synovitis (PVNS) are disorders associated with trisomy 7. The aim of the present study was to determine the frequency and distribution of the cells with +7 in vivo by analyzing sections of paraffin-embedded synovia from patients affected by OA, PVNS, other forms of synovitis [hemorragic synovitis (HS) and chronic synovitis (CS)], and from individuals without joint disease. Fluorescence in situ hybridization (FISH), using a centromeric probe for chromosome 7, showed that the mean frequency of trisomic nuclei in 5-microm sections was highest in PVNS (9.0%), followed by CS (5.9%), OA (5.6%), and HS (4.6%), whereas trisomic nuclei were rare (0.7%) in normal tissue. When 8-microm sections were studied, the frequencies of trisomic cells in OA and control synovia increased to 6.7% and 1.5%, respectively. Trisomic nuclei were found in all cases, including those for which cytogenetic analysis of short-term cultures had not disclosed any trisomic cells. Overall, the trisomic cells were scattered within the tissue. However, small clusters of cells with +7 were found in three cases. By hematoxylin-eosin staining of the slides used for FISH analysis it could be shown that the clustered trisomic cells were proliferating synoviocytes within villous extensions of the synovial membrane.

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published
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keywords
Adult, Aged, Aged, 80 and over, Cell Count, Chromosomes, Human, Pair 7, Data Interpretation, Statistical, Female, Humans, In Situ Hybridization, Fluorescence, Karyotyping, Male, Middle Aged, Osteoarthritis, Synovial Fluid, Synovitis, Pigmented Villonodular, Trisomy, Journal Article, Research Support, Non-U.S. Gov't
in
Cancer Genetics and Cytogenetics
volume
131
issue
1
pages
6 pages
publisher
Elsevier
external identifiers
  • pmid:11734313
  • scopus:0035187116
  • pmid:11734313
ISSN
0165-4608
DOI
10.1016/S0165-4608(01)00488-5
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Occupational and Environmental Medicine (013078001), Division of Clinical Genetics (013022003), Department of Orthopaedics (Lund) (013028000), Pathology, (Lund) (013030000)
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a1eaf076-050a-40f6-bf06-b2313279f554 (old id 1120103)
date added to LUP
2016-04-01 16:03:00
date last changed
2020-01-12 18:59:00
@article{a1eaf076-050a-40f6-bf06-b2313279f554,
  abstract     = {<p>Osteoarthritis (OA) and pigmented villonodular synovitis (PVNS) are disorders associated with trisomy 7. The aim of the present study was to determine the frequency and distribution of the cells with +7 in vivo by analyzing sections of paraffin-embedded synovia from patients affected by OA, PVNS, other forms of synovitis [hemorragic synovitis (HS) and chronic synovitis (CS)], and from individuals without joint disease. Fluorescence in situ hybridization (FISH), using a centromeric probe for chromosome 7, showed that the mean frequency of trisomic nuclei in 5-microm sections was highest in PVNS (9.0%), followed by CS (5.9%), OA (5.6%), and HS (4.6%), whereas trisomic nuclei were rare (0.7%) in normal tissue. When 8-microm sections were studied, the frequencies of trisomic cells in OA and control synovia increased to 6.7% and 1.5%, respectively. Trisomic nuclei were found in all cases, including those for which cytogenetic analysis of short-term cultures had not disclosed any trisomic cells. Overall, the trisomic cells were scattered within the tissue. However, small clusters of cells with +7 were found in three cases. By hematoxylin-eosin staining of the slides used for FISH analysis it could be shown that the clustered trisomic cells were proliferating synoviocytes within villous extensions of the synovial membrane.</p>},
  author       = {Dahlén, A and Broberg, K and Domanski, H A and Toksvig-Larsen, S and Lindstrand, A and Mandahl, N and Mertens, F},
  issn         = {0165-4608},
  language     = {eng},
  number       = {1},
  pages        = {19--24},
  publisher    = {Elsevier},
  series       = {Cancer Genetics and Cytogenetics},
  title        = {Analysis of the distribution and frequency of trisomy 7 in vivo in synovia from patients with osteoarthritis and pigmented villonodular synovitis},
  url          = {http://dx.doi.org/10.1016/S0165-4608(01)00488-5},
  doi          = {10.1016/S0165-4608(01)00488-5},
  volume       = {131},
  year         = {2001},
}