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DNA copy number amplifications in sarcomas with homogeneously staining regions and double minutes

Menghi-Sartorio, Samantha; Mandahl, Nils LU ; Mertens, Fredrik LU ; Picci, Piero and Knuutila, Sakari (2001) In Cytometry 46(2). p.79-84
Abstract
To identify DNA amplifications in sarcomas, comparative genomic hybridization was performed on 27 cases that were likely to display high-level DNA copy number gains. In all cases, chromosome banding analysis had revealed homogeneously staining regions or double minutes, i.e., cytogenetic signs of gene amplification. In most cases, gains predominated over losses. Low-level amplifications (ratio 1.3:1.5) were seen in 20 cases. High-level amplifications (ratio >1.5) exceeded the frequencies seen in published, unselected sarcomas of similar histotypes and were detected in 16 tumors: 4/4 osteosarcomas, 5/8 malignant fibrous histiocytomas, 3/7 leiomyosarcomas, 1/2 myosarcomas, 0/1 liposarcoma, 0/1 rhabdomyosarcoma, 1/1 pleomorphic sarcoma,... (More)
To identify DNA amplifications in sarcomas, comparative genomic hybridization was performed on 27 cases that were likely to display high-level DNA copy number gains. In all cases, chromosome banding analysis had revealed homogeneously staining regions or double minutes, i.e., cytogenetic signs of gene amplification. In most cases, gains predominated over losses. Low-level amplifications (ratio 1.3:1.5) were seen in 20 cases. High-level amplifications (ratio >1.5) exceeded the frequencies seen in published, unselected sarcomas of similar histotypes and were detected in 16 tumors: 4/4 osteosarcomas, 5/8 malignant fibrous histiocytomas, 3/7 leiomyosarcomas, 1/2 myosarcomas, 0/1 liposarcoma, 0/1 rhabdomyosarcoma, 1/1 pleomorphic sarcoma, 0/1 myxofibrosarcoma, 1/1 malignant mesenchymona, and 1/1 malignant schwannoma, with two to four chromosomal regions involved in nine tumors. Recurrent amplifications involved 1p33-p32, 5p15-p14, 7pter-p12, 7q21-qter, 8q21.3-qter, 11q22-q23, 16p13.2-p12, 19q12-q13.1, 20q11.2-qter, and 22q12-q13. Most of the recurrent gains/amplifications we detected have been reported in sarcomas previously. A novel gain/amplification was seen at 2q14.3-q21 in five cases of four sarcoma types. The disparate pattern of amplified sequences, the poor correspondence between the localization of low- and high-level amplifications, and the chromosomal position of homogeneously staining regions suggest the involvement of many genes in the amplifications and that the genes rarely maintain their native position in these tumors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
gene amplification, comparative genomic hybridization, homogeneously staining chromosomal region, double minute chromosome, chromosomal aberration, DNA copy number change
in
Cytometry
volume
46
issue
2
pages
79 - 84
publisher
John Wiley & Sons
external identifiers
  • pmid:11309816
  • scopus:0035870705
ISSN
0196-4763
DOI
10.1002/cyto.1068
language
English
LU publication?
yes
id
e111bd23-8da2-4ce2-baee-e2c2ea179ec4 (old id 1120305)
date added to LUP
2008-07-04 16:00:22
date last changed
2018-01-07 09:35:36
@article{e111bd23-8da2-4ce2-baee-e2c2ea179ec4,
  abstract     = {To identify DNA amplifications in sarcomas, comparative genomic hybridization was performed on 27 cases that were likely to display high-level DNA copy number gains. In all cases, chromosome banding analysis had revealed homogeneously staining regions or double minutes, i.e., cytogenetic signs of gene amplification. In most cases, gains predominated over losses. Low-level amplifications (ratio 1.3:1.5) were seen in 20 cases. High-level amplifications (ratio >1.5) exceeded the frequencies seen in published, unselected sarcomas of similar histotypes and were detected in 16 tumors: 4/4 osteosarcomas, 5/8 malignant fibrous histiocytomas, 3/7 leiomyosarcomas, 1/2 myosarcomas, 0/1 liposarcoma, 0/1 rhabdomyosarcoma, 1/1 pleomorphic sarcoma, 0/1 myxofibrosarcoma, 1/1 malignant mesenchymona, and 1/1 malignant schwannoma, with two to four chromosomal regions involved in nine tumors. Recurrent amplifications involved 1p33-p32, 5p15-p14, 7pter-p12, 7q21-qter, 8q21.3-qter, 11q22-q23, 16p13.2-p12, 19q12-q13.1, 20q11.2-qter, and 22q12-q13. Most of the recurrent gains/amplifications we detected have been reported in sarcomas previously. A novel gain/amplification was seen at 2q14.3-q21 in five cases of four sarcoma types. The disparate pattern of amplified sequences, the poor correspondence between the localization of low- and high-level amplifications, and the chromosomal position of homogeneously staining regions suggest the involvement of many genes in the amplifications and that the genes rarely maintain their native position in these tumors.},
  author       = {Menghi-Sartorio, Samantha and Mandahl, Nils and Mertens, Fredrik and Picci, Piero and Knuutila, Sakari},
  issn         = {0196-4763},
  keyword      = {gene amplification,comparative genomic hybridization,homogeneously staining chromosomal region,double minute chromosome,chromosomal aberration,DNA copy number change},
  language     = {eng},
  number       = {2},
  pages        = {79--84},
  publisher    = {John Wiley & Sons},
  series       = {Cytometry},
  title        = {DNA copy number amplifications in sarcomas with homogeneously staining regions and double minutes},
  url          = {http://dx.doi.org/10.1002/cyto.1068},
  volume       = {46},
  year         = {2001},
}