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No EWS/FLI1 fusion transcripts in giant-cell tumors of bone

Panagopoulos, Ioannis LU ; Mertens, Fredrik LU ; Domanski, Henryk LU ; Isaksson, Margareth LU ; Brosjö, Otte; Gustafson, Pelle LU and Mandahl, Nils LU (2001) In International Journal of Cancer 93(6). p.769-772
Abstract
Giant-cell tumor of bone (GCT) is a locally aggressive neoplasm of unknown etiology and pathogenesis. Cytogenetically, no consistent chromosomal alterations, apart from telomeric associations involving various chromosome ends, have been described. Recently, however, it was reported that by using highly sensitive nested RT-PCR, a high proportion of GCT displays chimeric EWS/FLI1 fusion transcripts, i.e., the molecular genetic feature previously known to be strongly associated with the Ewing family of tumors. Thus, we decided to perform single-step and nested RT-PCR analyses on fresh frozen samples from 10 cases of GCT, all of which had also been subjected to cytogenetic analysis. After short-term culturing, none of the samples displayed any... (More)
Giant-cell tumor of bone (GCT) is a locally aggressive neoplasm of unknown etiology and pathogenesis. Cytogenetically, no consistent chromosomal alterations, apart from telomeric associations involving various chromosome ends, have been described. Recently, however, it was reported that by using highly sensitive nested RT-PCR, a high proportion of GCT displays chimeric EWS/FLI1 fusion transcripts, i.e., the molecular genetic feature previously known to be strongly associated with the Ewing family of tumors. Thus, we decided to perform single-step and nested RT-PCR analyses on fresh frozen samples from 10 cases of GCT, all of which had also been subjected to cytogenetic analysis. After short-term culturing, none of the samples displayed any t(11;22)(q24;q12), the translocation characteristically giving rise to the EWS/FLI1 fusion, nor any other type of rearrangement of 11q24 or 22q12. Furthermore, in none of the cases did the RT-PCR analysis, whether single step or nested, result in products corresponding to a hybrid EWS/FLI1 transcript. On the basis of these results, we conclude that translocations leading to fusion of the EWS and FLI1 genes are not part of the pathogenesis of GCT. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
giant-cell tumor of bone, EWS, nested PCR, FLI1, gene fusion
in
International Journal of Cancer
volume
93
issue
6
pages
769 - 772
publisher
John Wiley & Sons
external identifiers
  • pmid:11519035
  • scopus:0035885264
ISSN
0020-7136
DOI
10.1002/ijc.1415
language
English
LU publication?
yes
id
aea85c14-f815-4df8-a10e-964349e17a8b (old id 1120339)
date added to LUP
2008-07-09 10:34:42
date last changed
2018-05-29 09:56:47
@article{aea85c14-f815-4df8-a10e-964349e17a8b,
  abstract     = {Giant-cell tumor of bone (GCT) is a locally aggressive neoplasm of unknown etiology and pathogenesis. Cytogenetically, no consistent chromosomal alterations, apart from telomeric associations involving various chromosome ends, have been described. Recently, however, it was reported that by using highly sensitive nested RT-PCR, a high proportion of GCT displays chimeric EWS/FLI1 fusion transcripts, i.e., the molecular genetic feature previously known to be strongly associated with the Ewing family of tumors. Thus, we decided to perform single-step and nested RT-PCR analyses on fresh frozen samples from 10 cases of GCT, all of which had also been subjected to cytogenetic analysis. After short-term culturing, none of the samples displayed any t(11;22)(q24;q12), the translocation characteristically giving rise to the EWS/FLI1 fusion, nor any other type of rearrangement of 11q24 or 22q12. Furthermore, in none of the cases did the RT-PCR analysis, whether single step or nested, result in products corresponding to a hybrid EWS/FLI1 transcript. On the basis of these results, we conclude that translocations leading to fusion of the EWS and FLI1 genes are not part of the pathogenesis of GCT.},
  author       = {Panagopoulos, Ioannis and Mertens, Fredrik and Domanski, Henryk and Isaksson, Margareth and Brosjö, Otte and Gustafson, Pelle and Mandahl, Nils},
  issn         = {0020-7136},
  keyword      = {giant-cell tumor of bone,EWS,nested PCR,FLI1,gene fusion},
  language     = {eng},
  number       = {6},
  pages        = {769--772},
  publisher    = {John Wiley & Sons},
  series       = {International Journal of Cancer},
  title        = {No EWS/FLI1 fusion transcripts in giant-cell tumors of bone},
  url          = {http://dx.doi.org/10.1002/ijc.1415},
  volume       = {93},
  year         = {2001},
}