No EWS/FLI1 fusion transcripts in giant-cell tumors of bone
(2001) In International Journal of Cancer 93(6). p.769-772- Abstract
- Giant-cell tumor of bone (GCT) is a locally aggressive neoplasm of unknown etiology and pathogenesis. Cytogenetically, no consistent chromosomal alterations, apart from telomeric associations involving various chromosome ends, have been described. Recently, however, it was reported that by using highly sensitive nested RT-PCR, a high proportion of GCT displays chimeric EWS/FLI1 fusion transcripts, i.e., the molecular genetic feature previously known to be strongly associated with the Ewing family of tumors. Thus, we decided to perform single-step and nested RT-PCR analyses on fresh frozen samples from 10 cases of GCT, all of which had also been subjected to cytogenetic analysis. After short-term culturing, none of the samples displayed any... (More)
- Giant-cell tumor of bone (GCT) is a locally aggressive neoplasm of unknown etiology and pathogenesis. Cytogenetically, no consistent chromosomal alterations, apart from telomeric associations involving various chromosome ends, have been described. Recently, however, it was reported that by using highly sensitive nested RT-PCR, a high proportion of GCT displays chimeric EWS/FLI1 fusion transcripts, i.e., the molecular genetic feature previously known to be strongly associated with the Ewing family of tumors. Thus, we decided to perform single-step and nested RT-PCR analyses on fresh frozen samples from 10 cases of GCT, all of which had also been subjected to cytogenetic analysis. After short-term culturing, none of the samples displayed any t(11;22)(q24;q12), the translocation characteristically giving rise to the EWS/FLI1 fusion, nor any other type of rearrangement of 11q24 or 22q12. Furthermore, in none of the cases did the RT-PCR analysis, whether single step or nested, result in products corresponding to a hybrid EWS/FLI1 transcript. On the basis of these results, we conclude that translocations leading to fusion of the EWS and FLI1 genes are not part of the pathogenesis of GCT. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1120339
- author
- Panagopoulos, Ioannis LU ; Mertens, Fredrik LU ; Domanski, Henryk LU ; Isaksson, Margareth LU ; Brosjö, Otte ; Gustafson, Pelle LU and Mandahl, Nils LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- giant-cell tumor of bone, EWS, nested PCR, FLI1, gene fusion
- in
- International Journal of Cancer
- volume
- 93
- issue
- 6
- pages
- 769 - 772
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:11519035
- scopus:0035885264
- ISSN
- 0020-7136
- DOI
- 10.1002/ijc.1415
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000), Division of Clinical Genetics (013022003), Department of Orthopaedics (Lund) (013028000)
- id
- aea85c14-f815-4df8-a10e-964349e17a8b (old id 1120339)
- date added to LUP
- 2016-04-01 11:41:03
- date last changed
- 2022-01-26 08:39:24
@article{aea85c14-f815-4df8-a10e-964349e17a8b, abstract = {{Giant-cell tumor of bone (GCT) is a locally aggressive neoplasm of unknown etiology and pathogenesis. Cytogenetically, no consistent chromosomal alterations, apart from telomeric associations involving various chromosome ends, have been described. Recently, however, it was reported that by using highly sensitive nested RT-PCR, a high proportion of GCT displays chimeric EWS/FLI1 fusion transcripts, i.e., the molecular genetic feature previously known to be strongly associated with the Ewing family of tumors. Thus, we decided to perform single-step and nested RT-PCR analyses on fresh frozen samples from 10 cases of GCT, all of which had also been subjected to cytogenetic analysis. After short-term culturing, none of the samples displayed any t(11;22)(q24;q12), the translocation characteristically giving rise to the EWS/FLI1 fusion, nor any other type of rearrangement of 11q24 or 22q12. Furthermore, in none of the cases did the RT-PCR analysis, whether single step or nested, result in products corresponding to a hybrid EWS/FLI1 transcript. On the basis of these results, we conclude that translocations leading to fusion of the EWS and FLI1 genes are not part of the pathogenesis of GCT.}}, author = {{Panagopoulos, Ioannis and Mertens, Fredrik and Domanski, Henryk and Isaksson, Margareth and Brosjö, Otte and Gustafson, Pelle and Mandahl, Nils}}, issn = {{0020-7136}}, keywords = {{giant-cell tumor of bone; EWS; nested PCR; FLI1; gene fusion}}, language = {{eng}}, number = {{6}}, pages = {{769--772}}, publisher = {{John Wiley & Sons Inc.}}, series = {{International Journal of Cancer}}, title = {{No EWS/FLI1 fusion transcripts in giant-cell tumors of bone}}, url = {{http://dx.doi.org/10.1002/ijc.1415}}, doi = {{10.1002/ijc.1415}}, volume = {{93}}, year = {{2001}}, }