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Determination of 5-hydroxy-N-methyl-2-pyrrolidone and 2-hydroxy-N-methylsuccinimide in human plasma and urine using liquid chromatography-electrospray tandem mass spectrometry

Carnerup, Martin LU ; Åkesson, Bengt LU and Jönsson, Bo A LU (2001) In Journal of Chromatography. B, Biomedical Sciences and Applications 761(1). p.107-113
Abstract
A method for simultaneous determination of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) was developed. These compounds are metabolites from N-methyl-2-pyrrolidone (NMP), a powerful and widely used organic solvent. 5-HNMP and 2-HMSI were purified from plasma and urine by solid-phase extraction using Isolute ENV+ columns, and analysed by liquid chromatography coupled to a mass spectrometer fitted with an atmospheric pressure turbo ion spray ionisation interface in the positive ion mode. The method was validated for plasma and urine concentrations from 0.12 to 25 microg/ml. The recoveries for 5-HNMP and 2-HMSI in plasma were 99 and 98%, respectively, and in urine 111 and 106%, respectively. For 5-HNMP... (More)
A method for simultaneous determination of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) was developed. These compounds are metabolites from N-methyl-2-pyrrolidone (NMP), a powerful and widely used organic solvent. 5-HNMP and 2-HMSI were purified from plasma and urine by solid-phase extraction using Isolute ENV+ columns, and analysed by liquid chromatography coupled to a mass spectrometer fitted with an atmospheric pressure turbo ion spray ionisation interface in the positive ion mode. The method was validated for plasma and urine concentrations from 0.12 to 25 microg/ml. The recoveries for 5-HNMP and 2-HMSI in plasma were 99 and 98%, respectively, and in urine 111 and 106%, respectively. For 5-HNMP and 2-HMSI, the within-day precision in plasma was 1-4 and 3-6%, respectively, and in urine 2-12 and 3-10%, respectively. The corresponding data for the between-day precision was 5 and 3-6%, respectively, and 4-6 and 7-8%, respectively. The detection limit for 5-HNMP was 4 ng/ml in plasma and 120 ng/ml in urine. For 2-HMSI, it was 5 ng/ml in plasma and 85 ng/ml in urine. The method is applicable for analysis of plasma and urine samples from workers exposed to NMP. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
5-Hydroxy-N-methyl-2-pyrrolidone, 2-Hydroxy-N-methylsuccinimide
in
Journal of Chromatography. B, Biomedical Sciences and Applications
volume
761
issue
1
pages
107 - 113
publisher
Elsevier
external identifiers
  • pmid:11585124
  • scopus:0035885241
ISSN
1387-2273
DOI
10.1016/S0378-4347(01)00312-7
language
English
LU publication?
yes
id
941bea92-203e-47b4-8b27-fd9b6d1d67cc (old id 1120673)
date added to LUP
2008-06-25 16:05:09
date last changed
2018-05-29 10:07:14
@article{941bea92-203e-47b4-8b27-fd9b6d1d67cc,
  abstract     = {A method for simultaneous determination of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) was developed. These compounds are metabolites from N-methyl-2-pyrrolidone (NMP), a powerful and widely used organic solvent. 5-HNMP and 2-HMSI were purified from plasma and urine by solid-phase extraction using Isolute ENV+ columns, and analysed by liquid chromatography coupled to a mass spectrometer fitted with an atmospheric pressure turbo ion spray ionisation interface in the positive ion mode. The method was validated for plasma and urine concentrations from 0.12 to 25 microg/ml. The recoveries for 5-HNMP and 2-HMSI in plasma were 99 and 98%, respectively, and in urine 111 and 106%, respectively. For 5-HNMP and 2-HMSI, the within-day precision in plasma was 1-4 and 3-6%, respectively, and in urine 2-12 and 3-10%, respectively. The corresponding data for the between-day precision was 5 and 3-6%, respectively, and 4-6 and 7-8%, respectively. The detection limit for 5-HNMP was 4 ng/ml in plasma and 120 ng/ml in urine. For 2-HMSI, it was 5 ng/ml in plasma and 85 ng/ml in urine. The method is applicable for analysis of plasma and urine samples from workers exposed to NMP.},
  author       = {Carnerup, Martin and Åkesson, Bengt and Jönsson, Bo A},
  issn         = {1387-2273},
  keyword      = {5-Hydroxy-N-methyl-2-pyrrolidone,2-Hydroxy-N-methylsuccinimide},
  language     = {eng},
  number       = {1},
  pages        = {107--113},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B, Biomedical Sciences and Applications},
  title        = {Determination of 5-hydroxy-N-methyl-2-pyrrolidone and 2-hydroxy-N-methylsuccinimide in human plasma and urine using liquid chromatography-electrospray tandem mass spectrometry},
  url          = {http://dx.doi.org/10.1016/S0378-4347(01)00312-7},
  volume       = {761},
  year         = {2001},
}