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Retinal integration of grafts of brain-derived precursor cell lines implanted subretinally into adult, normal rats

Warfvinge, Karin LU ; Kamme, Christina; Englund Johansson, Ulrica LU and Wictorin, Klas LU (2001) In Experimental Neurology 169(1). p.1-12
Abstract
The ability of in vitro-expanded neural precursor cells or cell lines to differentiate following transplantation has significant implications for current research on central nervous system repair. Recently, interest has been focussed on grafts of such neural precursors implanted also into the eye or retina. Here, we demonstrate with a non-traumatizing subretinal transplantation method, that grafts of the two immortalized brain-derived cell lines C 17-2 (from postnatal mouse cerebellum) and RN33B (from the embryonic rat medullary raphe) survive for at least up to four weeks, after implantation into the adult normal rat retina. For both cell lines, implanted cells gradually integrate into all major retinal cell layers, including the retinal... (More)
The ability of in vitro-expanded neural precursor cells or cell lines to differentiate following transplantation has significant implications for current research on central nervous system repair. Recently, interest has been focussed on grafts of such neural precursors implanted also into the eye or retina. Here, we demonstrate with a non-traumatizing subretinal transplantation method, that grafts of the two immortalized brain-derived cell lines C 17-2 (from postnatal mouse cerebellum) and RN33B (from the embryonic rat medullary raphe) survive for at least up to four weeks, after implantation into the adult normal rat retina. For both cell lines, implanted cells gradually integrate into all major retinal cell layers, including the retinal pigment epithelium, and judged by the morphology differentiate into both glial- and neuron-like cells, as shown by thymidine autoradiography, mouse-specific in situ hybridization, and using immunohistochemistry to detect the reporter gene LacZ. Our results suggest that these and other similar neural cell lines could be very useful in the continuous experiments in models of retinal disorders to further assess both the cell replacement and ex vivo gene therapy approaches. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Neurology
volume
169
issue
1
pages
1 - 12
publisher
Academic Press
external identifiers
  • pmid:11312552
  • scopus:0035004933
ISSN
0014-4886
DOI
10.1006/exnr.2001.7661
language
English
LU publication?
yes
id
62b2bd81-75f0-4171-abfc-776463f35429 (old id 1120771)
date added to LUP
2008-07-17 11:09:20
date last changed
2018-05-29 11:54:35
@article{62b2bd81-75f0-4171-abfc-776463f35429,
  abstract     = {The ability of in vitro-expanded neural precursor cells or cell lines to differentiate following transplantation has significant implications for current research on central nervous system repair. Recently, interest has been focussed on grafts of such neural precursors implanted also into the eye or retina. Here, we demonstrate with a non-traumatizing subretinal transplantation method, that grafts of the two immortalized brain-derived cell lines C 17-2 (from postnatal mouse cerebellum) and RN33B (from the embryonic rat medullary raphe) survive for at least up to four weeks, after implantation into the adult normal rat retina. For both cell lines, implanted cells gradually integrate into all major retinal cell layers, including the retinal pigment epithelium, and judged by the morphology differentiate into both glial- and neuron-like cells, as shown by thymidine autoradiography, mouse-specific in situ hybridization, and using immunohistochemistry to detect the reporter gene LacZ. Our results suggest that these and other similar neural cell lines could be very useful in the continuous experiments in models of retinal disorders to further assess both the cell replacement and ex vivo gene therapy approaches.},
  author       = {Warfvinge, Karin and Kamme, Christina and Englund Johansson, Ulrica and Wictorin, Klas},
  issn         = {0014-4886},
  language     = {eng},
  number       = {1},
  pages        = {1--12},
  publisher    = {Academic Press},
  series       = {Experimental Neurology},
  title        = {Retinal integration of grafts of brain-derived precursor cell lines implanted subretinally into adult, normal rats},
  url          = {http://dx.doi.org/10.1006/exnr.2001.7661},
  volume       = {169},
  year         = {2001},
}