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Regeneration of detrusor muscle after subtotal cystectomy in the rat; effects on contractile proteins and bladder mechanics.

Frederiksen, Hans LU ; Sjuve, Rolf; Arner, Anders LU and Uvelius, Bengt LU (2001) In Neurourology and Urodynamics 20(6). p.685-697
Abstract
The aim of the present study was to determine to what extent adult rats can produce new contracting bladder muscle and to see if such newly formed bladder tissue possesses characteristic mechanical properties or whether the ability to recover mechanically is so pronounced that the prehistory of the bladder is unimportant. Subtotal cystectomy was performed in adult female rats, leading to a pronounced decrease in total bladder weight. At 10 weeks, bladder weight had normalized. The histological appearance of such bladders was similar to that of the controls. Active and passive length-tension relations for the detrusor muscle were determined in controls and up to 10 weeks after surgery. Immediately after surgery active and passive forces... (More)
The aim of the present study was to determine to what extent adult rats can produce new contracting bladder muscle and to see if such newly formed bladder tissue possesses characteristic mechanical properties or whether the ability to recover mechanically is so pronounced that the prehistory of the bladder is unimportant. Subtotal cystectomy was performed in adult female rats, leading to a pronounced decrease in total bladder weight. At 10 weeks, bladder weight had normalized. The histological appearance of such bladders was similar to that of the controls. Active and passive length-tension relations for the detrusor muscle were determined in controls and up to 10 weeks after surgery. Immediately after surgery active and passive forces showed a leftward shift and maximum active force decreased markedly. With time the length-tension curves shifted back to normal, but a decreased active force still remained at 10 weeks. Detrusor actin concentration and detrusor myosin/actin ratio were unaffected by the subtotal cystectomy. Intermediate filament protein/actin ratio showed a significant but transitory increase. We conclude that there is a remarkable recovery of detrusor muscle function after subtotal cystectomy, leading to a normalization of optimum length for active force and a net synthesis of contractile and cytoskeletal proteins. The ability to produce active force does, however, not fully recover. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
urinary bladder, detrusor, smooth muscle, rat, length-tension relations, cystectomy, regeneration
in
Neurourology and Urodynamics
volume
20
issue
6
pages
685 - 697
publisher
John Wiley & Sons
external identifiers
  • wos:000171745100007
  • scopus:0034777979
ISSN
0733-2467
DOI
10.1002/nau.1020
language
English
LU publication?
yes
id
5e358632-3bf2-489b-b7b1-8396afb0cb7c (old id 1121008)
date added to LUP
2008-06-27 11:59:26
date last changed
2018-01-07 05:07:46
@article{5e358632-3bf2-489b-b7b1-8396afb0cb7c,
  abstract     = {The aim of the present study was to determine to what extent adult rats can produce new contracting bladder muscle and to see if such newly formed bladder tissue possesses characteristic mechanical properties or whether the ability to recover mechanically is so pronounced that the prehistory of the bladder is unimportant. Subtotal cystectomy was performed in adult female rats, leading to a pronounced decrease in total bladder weight. At 10 weeks, bladder weight had normalized. The histological appearance of such bladders was similar to that of the controls. Active and passive length-tension relations for the detrusor muscle were determined in controls and up to 10 weeks after surgery. Immediately after surgery active and passive forces showed a leftward shift and maximum active force decreased markedly. With time the length-tension curves shifted back to normal, but a decreased active force still remained at 10 weeks. Detrusor actin concentration and detrusor myosin/actin ratio were unaffected by the subtotal cystectomy. Intermediate filament protein/actin ratio showed a significant but transitory increase. We conclude that there is a remarkable recovery of detrusor muscle function after subtotal cystectomy, leading to a normalization of optimum length for active force and a net synthesis of contractile and cytoskeletal proteins. The ability to produce active force does, however, not fully recover.},
  author       = {Frederiksen, Hans and Sjuve, Rolf and Arner, Anders and Uvelius, Bengt},
  issn         = {0733-2467},
  keyword      = {urinary bladder,detrusor,smooth muscle,rat,length-tension relations,cystectomy,regeneration},
  language     = {eng},
  number       = {6},
  pages        = {685--697},
  publisher    = {John Wiley & Sons},
  series       = {Neurourology and Urodynamics},
  title        = {Regeneration of detrusor muscle after subtotal cystectomy in the rat; effects on contractile proteins and bladder mechanics.},
  url          = {http://dx.doi.org/10.1002/nau.1020},
  volume       = {20},
  year         = {2001},
}