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The fate of mechanically induced cartilage in an unloaded environment

de Rooij, Philippe P.; Siebrecht, Maikel A. N.; Tägil, Magnus LU and Aspenberg, Per LU (2001) In Journal of Biomechanics 34(7). p.961-966
Abstract
According to mechanobiologic theories, persistent intermittent mechanical stimulation is required to maintain differentiated cartilage. In a rat model for bone repair, we studied the fate of mechanically induced cartilage after unloading. In three groups of rats, regenerating mesenchymal tissue was submitted to different loading conditions in bone chambers. Two groups were immediately killed after loading periods of 3 or 6 weeks (the 3-group and the 6-group). The third group was loaded for 3 weeks and then kept unloaded for another 3 weeks (the (3 + 3)-group). Cartilage was found in all loaded groups. Without loading, cartilage does not appear in this model. In the 3-group there was no clear ongoing endochondral ossification, the 6-group... (More)
According to mechanobiologic theories, persistent intermittent mechanical stimulation is required to maintain differentiated cartilage. In a rat model for bone repair, we studied the fate of mechanically induced cartilage after unloading. In three groups of rats, regenerating mesenchymal tissue was submitted to different loading conditions in bone chambers. Two groups were immediately killed after loading periods of 3 or 6 weeks (the 3-group and the 6-group). The third group was loaded for 3 weeks and then kept unloaded for another 3 weeks (the (3 + 3)-group). Cartilage was found in all loaded groups. Without loading, cartilage does not appear in this model. In the 3-group there was no clear ongoing endochondral ossification, the 6-group showed ossification in 2 out of 5 cartilage containing specimens, and in the (3 + 3)-group all cartilage was undergoing ossification. These results suggest a tendency of the cartilage to be maintained also under unloaded conditions until it is reached by bone that can replace it through endochondral ossification.Additional measurements showed less amount of new bone in the loaded specimens. In most of the loaded specimens in the 3-group, necrotic bone fragments were seen embedded in the fibrous tissue layer close to the loading piston, indicating that bone tissue had been resorbed due to the hydrostatic compressive load. In some specimens, a continuous cartilage layer covered the end of the specimen and seemed to protect the underlying bone from pressure-induced resorption. We suggest that one of the functions of the cartilage forming in the compressive loaded parts of a bone callus is to protect the surrounding bone callus from pressure-induced fluid flow leading to resorption. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Tissue differentiation, Mechanical load, Endochondral ossification, Fluid pressure
in
Journal of Biomechanics
volume
34
issue
7
pages
961 - 966
publisher
Elsevier
external identifiers
  • pmid:11410179
  • scopus:0034983364
ISSN
1873-2380
DOI
10.1016/S0021-9290(01)00044-6
language
English
LU publication?
yes
id
7d0c1185-777e-41e3-81e4-40ed6fd56cb2 (old id 1121338)
date added to LUP
2008-06-26 12:02:46
date last changed
2018-05-29 11:36:55
@article{7d0c1185-777e-41e3-81e4-40ed6fd56cb2,
  abstract     = {According to mechanobiologic theories, persistent intermittent mechanical stimulation is required to maintain differentiated cartilage. In a rat model for bone repair, we studied the fate of mechanically induced cartilage after unloading. In three groups of rats, regenerating mesenchymal tissue was submitted to different loading conditions in bone chambers. Two groups were immediately killed after loading periods of 3 or 6 weeks (the 3-group and the 6-group). The third group was loaded for 3 weeks and then kept unloaded for another 3 weeks (the (3 + 3)-group). Cartilage was found in all loaded groups. Without loading, cartilage does not appear in this model. In the 3-group there was no clear ongoing endochondral ossification, the 6-group showed ossification in 2 out of 5 cartilage containing specimens, and in the (3 + 3)-group all cartilage was undergoing ossification. These results suggest a tendency of the cartilage to be maintained also under unloaded conditions until it is reached by bone that can replace it through endochondral ossification.Additional measurements showed less amount of new bone in the loaded specimens. In most of the loaded specimens in the 3-group, necrotic bone fragments were seen embedded in the fibrous tissue layer close to the loading piston, indicating that bone tissue had been resorbed due to the hydrostatic compressive load. In some specimens, a continuous cartilage layer covered the end of the specimen and seemed to protect the underlying bone from pressure-induced resorption. We suggest that one of the functions of the cartilage forming in the compressive loaded parts of a bone callus is to protect the surrounding bone callus from pressure-induced fluid flow leading to resorption.},
  author       = {de Rooij, Philippe P. and Siebrecht, Maikel A. N. and Tägil, Magnus and Aspenberg, Per},
  issn         = {1873-2380},
  keyword      = {Tissue differentiation,Mechanical load,Endochondral ossification,Fluid pressure},
  language     = {eng},
  number       = {7},
  pages        = {961--966},
  publisher    = {Elsevier},
  series       = {Journal of Biomechanics},
  title        = {The fate of mechanically induced cartilage in an unloaded environment},
  url          = {http://dx.doi.org/10.1016/S0021-9290(01)00044-6},
  volume       = {34},
  year         = {2001},
}