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Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats

Kaastad, T S; Reikeras, O; Halvorsen, V; Falch, J A; Obrant, Karl LU and Nordsletten, L (2001) In Calcified Tissue International 69(2). p.102-108
Abstract
Vitamin D (vit D) deficiency is common in the elderly, and the aim of this study was to investigate whether vit D deprivation in ovariectomized (ovx) and normal rats would reduce fracture strength. Forty mature female Wistar rats were randomized into four groups: two were ovariectomized (ovx) and two were sham-operated (sham). One ovx and one sham group were fed a vit D-deficient diet (Ovx-D and Sham-D), and the control groups were fed normal rat chow (Ovx and Sham) for 12 weeks. Vit D deficiency was substantiated after 12 weeks by undetectable serum concentrations of 25OHD in the Sham-D and Ovx-D groups. 85Sr activity was lower in Sham-D than in the other groups (P < 0.005). Tibial and femoral weights and lengths showed no differences.... (More)
Vitamin D (vit D) deficiency is common in the elderly, and the aim of this study was to investigate whether vit D deprivation in ovariectomized (ovx) and normal rats would reduce fracture strength. Forty mature female Wistar rats were randomized into four groups: two were ovariectomized (ovx) and two were sham-operated (sham). One ovx and one sham group were fed a vit D-deficient diet (Ovx-D and Sham-D), and the control groups were fed normal rat chow (Ovx and Sham) for 12 weeks. Vit D deficiency was substantiated after 12 weeks by undetectable serum concentrations of 25OHD in the Sham-D and Ovx-D groups. 85Sr activity was lower in Sham-D than in the other groups (P < 0.005). Tibial and femoral weights and lengths showed no differences. Distal tibial trabecular bone volume was reduced in both ovx groups compare with sham (P < 0.005). Bone mineral density (BMD) was higher in sham than in Sham-D and both ovx groups (P < 0.005). Femoral area moment of inertia increased and ultimate stress decreased in Ovx-D compared with ovx (P < 0.05). Other biomechanical properties of the femoral shafts did not differ significantly. The femoral neck was significantly weaker in Ovx-D than in the other groups. In conclusion, ovx decreased tibial trabecular bone volume and both ovx and vit D depletion reduced femoral BMD in rats. Vit D depletion reduced the ultimate stress in the femoral shaft, and the combined depletion of estrogen and vit D significantly reduced the fracture strength in the femoral neck. This fits well with clinical evidence of how postmenopausal status combined with vit D deficiency lead to an increased risk of hip fractures, making this animal model a possible tool for investigating measures to prevent such fractures. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Calcified Tissue International
volume
69
issue
2
pages
102 - 108
publisher
Springer
external identifiers
  • pmid:11683422
  • scopus:0034887490
ISSN
1432-0827
DOI
10.1007/s00223-001-0009-2
language
English
LU publication?
yes
id
eef03470-2d5a-4693-a4a9-81851a569fb7 (old id 1121547)
date added to LUP
2008-07-01 10:52:23
date last changed
2018-01-07 08:36:47
@article{eef03470-2d5a-4693-a4a9-81851a569fb7,
  abstract     = {Vitamin D (vit D) deficiency is common in the elderly, and the aim of this study was to investigate whether vit D deprivation in ovariectomized (ovx) and normal rats would reduce fracture strength. Forty mature female Wistar rats were randomized into four groups: two were ovariectomized (ovx) and two were sham-operated (sham). One ovx and one sham group were fed a vit D-deficient diet (Ovx-D and Sham-D), and the control groups were fed normal rat chow (Ovx and Sham) for 12 weeks. Vit D deficiency was substantiated after 12 weeks by undetectable serum concentrations of 25OHD in the Sham-D and Ovx-D groups. 85Sr activity was lower in Sham-D than in the other groups (P &lt; 0.005). Tibial and femoral weights and lengths showed no differences. Distal tibial trabecular bone volume was reduced in both ovx groups compare with sham (P &lt; 0.005). Bone mineral density (BMD) was higher in sham than in Sham-D and both ovx groups (P &lt; 0.005). Femoral area moment of inertia increased and ultimate stress decreased in Ovx-D compared with ovx (P &lt; 0.05). Other biomechanical properties of the femoral shafts did not differ significantly. The femoral neck was significantly weaker in Ovx-D than in the other groups. In conclusion, ovx decreased tibial trabecular bone volume and both ovx and vit D depletion reduced femoral BMD in rats. Vit D depletion reduced the ultimate stress in the femoral shaft, and the combined depletion of estrogen and vit D significantly reduced the fracture strength in the femoral neck. This fits well with clinical evidence of how postmenopausal status combined with vit D deficiency lead to an increased risk of hip fractures, making this animal model a possible tool for investigating measures to prevent such fractures.},
  author       = {Kaastad, T S and Reikeras, O and Halvorsen, V and Falch, J A and Obrant, Karl and Nordsletten, L},
  issn         = {1432-0827},
  language     = {eng},
  number       = {2},
  pages        = {102--108},
  publisher    = {Springer},
  series       = {Calcified Tissue International},
  title        = {Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats},
  url          = {http://dx.doi.org/10.1007/s00223-001-0009-2},
  volume       = {69},
  year         = {2001},
}