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Gender and strain influence on neurogenesis in dentate gyrus of young rats

Perfilieva, Ekaterina ; Risedal, Anette LU ; Nyberg, Jenny ; Johansson, Barbro LU and Eriksson, Peter S (2001) In Journal of Cerebral Blood Flow and Metabolism 21(3). p.211-217
Abstract
To investigate whether rat hippocampal neurogenesis varies with strain and gender, the authors examined proliferating progenitor cells and their progeny in young male and female Sprague-Dawley (SD) and spontaneously hypertensive rats (SHR) using the thymidine analog bromodeoxyuridine (BrdU) combined with immunohistochemistry for the neuronal marker Calbindin D28k and glial fibrillary acidic protein. Rats were given 7 consecutive daily BrdU injections and were killed 1 day or 4 weeks later to allow for discrimination between proliferation and cell survival. Stereologic analysis of the numbers of BrdU-immunoreactive cells in the dentate gyrus revealed both a strain difference with significantly higher cell proliferation and net neurogenesis... (More)
To investigate whether rat hippocampal neurogenesis varies with strain and gender, the authors examined proliferating progenitor cells and their progeny in young male and female Sprague-Dawley (SD) and spontaneously hypertensive rats (SHR) using the thymidine analog bromodeoxyuridine (BrdU) combined with immunohistochemistry for the neuronal marker Calbindin D28k and glial fibrillary acidic protein. Rats were given 7 consecutive daily BrdU injections and were killed 1 day or 4 weeks later to allow for discrimination between proliferation and cell survival. Stereologic analysis of the numbers of BrdU-immunoreactive cells in the dentate gyrus revealed both a strain difference with significantly higher cell proliferation and net neurogenesis in SHR than in SD and a gender difference with males from both strains producing significantly more cells than their female counterparts. Whereas the number of progenitors four weeks after BrdU injections was still significantly greater in male than in female SHRs, resulting in a greater net neurogenesis in the male, the number of BrdU-immunoreactive cells did not differ between male and female SD rats, suggesting a greater survival of newly generated cells in the dentate gyrus in female than in male SD rats. No sex or strain difference was observed in the relative ratio of neurogenesis and gliogenesis. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Neurogenesis, Progenitor cells, Gender difference, Strain difference, SHR, Sprague-Dawley
in
Journal of Cerebral Blood Flow and Metabolism
volume
21
issue
3
pages
211 - 217
publisher
Nature Publishing Group
external identifiers
  • pmid:11295875
  • scopus:0035098306
ISSN
1559-7016
DOI
10.1097/00004647-200103000-00004
language
English
LU publication?
yes
id
644e46e8-c465-406a-b23e-3f4eeaf0cf8e (old id 1121943)
date added to LUP
2016-04-01 17:10:50
date last changed
2025-04-04 15:02:47
@article{644e46e8-c465-406a-b23e-3f4eeaf0cf8e,
  abstract     = {{To investigate whether rat hippocampal neurogenesis varies with strain and gender, the authors examined proliferating progenitor cells and their progeny in young male and female Sprague-Dawley (SD) and spontaneously hypertensive rats (SHR) using the thymidine analog bromodeoxyuridine (BrdU) combined with immunohistochemistry for the neuronal marker Calbindin D28k and glial fibrillary acidic protein. Rats were given 7 consecutive daily BrdU injections and were killed 1 day or 4 weeks later to allow for discrimination between proliferation and cell survival. Stereologic analysis of the numbers of BrdU-immunoreactive cells in the dentate gyrus revealed both a strain difference with significantly higher cell proliferation and net neurogenesis in SHR than in SD and a gender difference with males from both strains producing significantly more cells than their female counterparts. Whereas the number of progenitors four weeks after BrdU injections was still significantly greater in male than in female SHRs, resulting in a greater net neurogenesis in the male, the number of BrdU-immunoreactive cells did not differ between male and female SD rats, suggesting a greater survival of newly generated cells in the dentate gyrus in female than in male SD rats. No sex or strain difference was observed in the relative ratio of neurogenesis and gliogenesis.}},
  author       = {{Perfilieva, Ekaterina and Risedal, Anette and Nyberg, Jenny and Johansson, Barbro and Eriksson, Peter S}},
  issn         = {{1559-7016}},
  keywords     = {{Neurogenesis; Progenitor cells; Gender difference; Strain difference; SHR; Sprague-Dawley}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{211--217}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Journal of Cerebral Blood Flow and Metabolism}},
  title        = {{Gender and strain influence on neurogenesis in dentate gyrus of young rats}},
  url          = {{http://dx.doi.org/10.1097/00004647-200103000-00004}},
  doi          = {{10.1097/00004647-200103000-00004}},
  volume       = {{21}},
  year         = {{2001}},
}