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Neuronal and fibre organization in neocortical grafts placed in post-ischaemic adult rat brain: a three-dimensional confocal microscopy study

Belichenko, P V; Mattsson, Bengt LU and Johansson, Barbro LU (2001) In Journal of Comparative Pathology 124(2-3). p.142-148
Abstract
The dendritic morphology in neocortical grafts was studied with three-dimensional confocal laser scanning microscopy after microinjection of Lucifer Yellow into individual cells. The grafts had been implanted into infarct cavities in the neocortex of hypertensive rats 46 weeks earlier. The carbocyanine dye method was used to identify afferent (host to transplant) and efferent (transplant to host) connections. Pyramidal, nonpyramidal and glial cells were present in the transplants. Some dendrites had an almost normal appearance, but abnormalities (atypical orientation of apical, basal or oblique apical dendrites) were observed. Some bi-apical pyramidal neurons and pyramidal neurons with obliquely oriented apical dendrites were also... (More)
The dendritic morphology in neocortical grafts was studied with three-dimensional confocal laser scanning microscopy after microinjection of Lucifer Yellow into individual cells. The grafts had been implanted into infarct cavities in the neocortex of hypertensive rats 46 weeks earlier. The carbocyanine dye method was used to identify afferent (host to transplant) and efferent (transplant to host) connections. Pyramidal, nonpyramidal and glial cells were present in the transplants. Some dendrites had an almost normal appearance, but abnormalities (atypical orientation of apical, basal or oblique apical dendrites) were observed. Some bi-apical pyramidal neurons and pyramidal neurons with obliquely oriented apical dendrites were also observed. Carbocyanine dye-labelled fibres of different diameter formed a dense network in the transplant, enabling the border between transplant and host tissue to be clearly recognized. No labelled fibres were observed to enter the host brain. Fibres with "boutons en passant" and no preferential orientation were noted. It is proposed that Lucifer Yellow microinjection may be a useful method in studies aimed at improving graft morphology. Failure to demonstrate host to transplant connections with the carbocyanine dye method was contrary to earlier studies in which tracers were applied in vivo. A combined use of in-vivo and post-mortem tracer techniques is needed to establish the reason for the discrepancy. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Comparative Pathology
volume
124
issue
2-3
pages
142 - 148
publisher
Elsevier
external identifiers
  • pmid:11222011
  • scopus:0034936745
ISSN
1532-3129
DOI
10.1053/jcpa.2000.0444
language
English
LU publication?
yes
id
b5d446d5-7bff-41cf-9666-fea6cbd23848 (old id 1121949)
date added to LUP
2008-06-24 12:57:27
date last changed
2018-01-07 05:24:00
@article{b5d446d5-7bff-41cf-9666-fea6cbd23848,
  abstract     = {The dendritic morphology in neocortical grafts was studied with three-dimensional confocal laser scanning microscopy after microinjection of Lucifer Yellow into individual cells. The grafts had been implanted into infarct cavities in the neocortex of hypertensive rats 46 weeks earlier. The carbocyanine dye method was used to identify afferent (host to transplant) and efferent (transplant to host) connections. Pyramidal, nonpyramidal and glial cells were present in the transplants. Some dendrites had an almost normal appearance, but abnormalities (atypical orientation of apical, basal or oblique apical dendrites) were observed. Some bi-apical pyramidal neurons and pyramidal neurons with obliquely oriented apical dendrites were also observed. Carbocyanine dye-labelled fibres of different diameter formed a dense network in the transplant, enabling the border between transplant and host tissue to be clearly recognized. No labelled fibres were observed to enter the host brain. Fibres with "boutons en passant" and no preferential orientation were noted. It is proposed that Lucifer Yellow microinjection may be a useful method in studies aimed at improving graft morphology. Failure to demonstrate host to transplant connections with the carbocyanine dye method was contrary to earlier studies in which tracers were applied in vivo. A combined use of in-vivo and post-mortem tracer techniques is needed to establish the reason for the discrepancy.},
  author       = {Belichenko, P V and Mattsson, Bengt and Johansson, Barbro},
  issn         = {1532-3129},
  language     = {eng},
  number       = {2-3},
  pages        = {142--148},
  publisher    = {Elsevier},
  series       = {Journal of Comparative Pathology},
  title        = {Neuronal and fibre organization in neocortical grafts placed in post-ischaemic adult rat brain: a three-dimensional confocal microscopy study},
  url          = {http://dx.doi.org/10.1053/jcpa.2000.0444},
  volume       = {124},
  year         = {2001},
}