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Electrically mediated drug delivery for treatment of an adenocarcinoma transplanted into rat liver

Engstrom, P.E.; Ivarsson, K.; Tranberg, K.G.; Stenram, Unne LU ; Salford, L.G. and Persson, B.R.R. (2001) In Anticancer research 21(3B). p.1817-1822
Abstract

BACKGROUND: In this study, electrochemotherapy (ECT), i.e. tumour treatment based on local augmentation of intracellular drug delivery from short, intense electric pulses, was evaluated in rats with an adenocarcinoma implanted into the liver. Tumour response and concentrations of macrophages and T-lymphocytes (CD4 and CD8) in and around the tumour were measured.

MATERIALS AND METHODS: Rats were treated with permeabilizing electric pulses, bleomycin, or both, eight days after implantation of the tumour, while one group received sham treatment.

RESULTS: Treatment with electric pulses and bleomycin resulted in a significantly reduced lesion volume and 92% cure rate (12 out of 13, p<0.0002 compared to the other treatment... (More)

BACKGROUND: In this study, electrochemotherapy (ECT), i.e. tumour treatment based on local augmentation of intracellular drug delivery from short, intense electric pulses, was evaluated in rats with an adenocarcinoma implanted into the liver. Tumour response and concentrations of macrophages and T-lymphocytes (CD4 and CD8) in and around the tumour were measured.

MATERIALS AND METHODS: Rats were treated with permeabilizing electric pulses, bleomycin, or both, eight days after implantation of the tumour, while one group received sham treatment.

RESULTS: Treatment with electric pulses and bleomycin resulted in a significantly reduced lesion volume and 92% cure rate (12 out of 13, p<0.0002 compared to the other treatment groups). The highest concentration of CD8 lymphocytes was found in tumours treated with electric pulses and bleomycin. Macrophages were found mainly in tumours treated with electric pulses, with or without bleomycin.

CONCLUSION: Electrochemotherapy using millisecond exponential pulses and bleomycin is efficient in a rat liver tumour model and appears to stimulate the host's immune system.

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organization
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published
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keywords
Adenocarcinoma, Alanine Transaminase, Animals, Antimetabolites, Antineoplastic, Bleomycin, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Combined Modality Therapy, Drug Delivery Systems, Electric Stimulation Therapy, Electroporation, Immunohistochemistry, Liver, Macrophages, Male, Neoplasm Transplantation, Rats, Rats, Wistar, Journal Article, Research Support, Non-U.S. Gov't
in
Anticancer research
volume
21
issue
3B
pages
6 pages
publisher
International Institute of Cancer Research
external identifiers
  • scopus:0034897388
ISSN
1791-7530
language
English
LU publication?
yes
id
b19d5306-088b-4c68-9913-a818c3abaab7 (old id 1122169)
date added to LUP
2008-06-26 16:41:02
date last changed
2018-10-03 10:12:19
@article{b19d5306-088b-4c68-9913-a818c3abaab7,
  abstract     = {<p>BACKGROUND: In this study, electrochemotherapy (ECT), i.e. tumour treatment based on local augmentation of intracellular drug delivery from short, intense electric pulses, was evaluated in rats with an adenocarcinoma implanted into the liver. Tumour response and concentrations of macrophages and T-lymphocytes (CD4 and CD8) in and around the tumour were measured.</p><p>MATERIALS AND METHODS: Rats were treated with permeabilizing electric pulses, bleomycin, or both, eight days after implantation of the tumour, while one group received sham treatment.</p><p>RESULTS: Treatment with electric pulses and bleomycin resulted in a significantly reduced lesion volume and 92% cure rate (12 out of 13, p&lt;0.0002 compared to the other treatment groups). The highest concentration of CD8 lymphocytes was found in tumours treated with electric pulses and bleomycin. Macrophages were found mainly in tumours treated with electric pulses, with or without bleomycin.</p><p>CONCLUSION: Electrochemotherapy using millisecond exponential pulses and bleomycin is efficient in a rat liver tumour model and appears to stimulate the host's immune system.</p>},
  author       = {Engstrom, P.E. and Ivarsson, K. and Tranberg, K.G. and Stenram, Unne and Salford, L.G. and Persson, B.R.R.},
  issn         = {1791-7530},
  keyword      = {Adenocarcinoma,Alanine Transaminase,Animals,Antimetabolites, Antineoplastic,Bleomycin,CD4-Positive T-Lymphocytes,CD8-Positive T-Lymphocytes,Combined Modality Therapy,Drug Delivery Systems,Electric Stimulation Therapy,Electroporation,Immunohistochemistry,Liver,Macrophages,Male,Neoplasm Transplantation,Rats,Rats, Wistar,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {08},
  number       = {3B},
  pages        = {1817--1822},
  publisher    = {International Institute of Cancer Research},
  series       = {Anticancer research},
  title        = {Electrically mediated drug delivery for treatment of an adenocarcinoma transplanted into rat liver},
  volume       = {21},
  year         = {2001},
}