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Increased expression of non-muscle myosin heavy chain-B in connective tissue cells of hypertrophic rat urinary bladder

Sjuve, Rolf; Haase, Hannelore; Ekblad, Eva LU ; Malmqvist, Ulf LU ; Morano, Ingo and Arner, Anders LU (2001) In Cell and Tissue Research1974-01-01+01:00 304(2). p.271-278
Abstract
Expression of the non-muscle myosin heavy chain-B (NM-MHC-B, also denoted as the embryonic smooth muscle myosin heavy chain, SMemb) was examined in rat urinary bladder during growth in response to a partial urinary outflow obstruction. Following obstruction, the weight of the urinary bladder increased more than five-fold within 10 days. Immunohistochemistry with a polyclonal antiserum against the C-terminal sequence of NM-MHC-B revealed very few NM-MHC-B immunoreactive cells in the control urinary bladders. In hypertrophic bladders, the number of NM-MHC-B immunoreactive cells markedly increased. The majority of such cells were found in the interstitium surrounding smooth muscle bundles and also in the subserosal and submucosal layers.... (More)
Expression of the non-muscle myosin heavy chain-B (NM-MHC-B, also denoted as the embryonic smooth muscle myosin heavy chain, SMemb) was examined in rat urinary bladder during growth in response to a partial urinary outflow obstruction. Following obstruction, the weight of the urinary bladder increased more than five-fold within 10 days. Immunohistochemistry with a polyclonal antiserum against the C-terminal sequence of NM-MHC-B revealed very few NM-MHC-B immunoreactive cells in the control urinary bladders. In hypertrophic bladders, the number of NM-MHC-B immunoreactive cells markedly increased. The majority of such cells were found in the interstitium surrounding smooth muscle bundles and also in the subserosal and submucosal layers. Western blot analysis showed that the NM-MHC-B expression was transient; the content of NM-MHC-B immunoreactive material had doubled 10 days after obstruction and then declined towards the control level after 6 weeks. Immunohistochemistry revealed co-localization of NM-MHC-B and vimentin within the same cells. NM-MHC-B did not co-localize with smooth muscle actin, suggesting that the source of NM-MHC-B is not a de-differentiated smooth muscle cell or myofibroblast but a non-muscle cell possibly reacting to tissue distension or stress. The NM-MHC-B-positive cells could have a role in the production of extracellular matrix and growth factors or be involved in modulation of spontaneous contractile activity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hypertrophy, Urinary bladder, Non-muscle myosin heavy chain-B (SMemb), Myosin, Rat (Sprague Dawley, female)
in
Cell and Tissue Research1974-01-01+01:00
volume
304
issue
2
pages
271 - 278
publisher
Springer
external identifiers
  • pmid:11396720
  • scopus:0035038958
ISSN
1432-0878
DOI
10.1007/s004410000262
language
English
LU publication?
yes
id
53fbbf17-274b-4ef1-87f0-3005135ff1d5 (old id 1122479)
date added to LUP
2008-07-15 09:10:34
date last changed
2018-01-07 05:14:44
@article{53fbbf17-274b-4ef1-87f0-3005135ff1d5,
  abstract     = {Expression of the non-muscle myosin heavy chain-B (NM-MHC-B, also denoted as the embryonic smooth muscle myosin heavy chain, SMemb) was examined in rat urinary bladder during growth in response to a partial urinary outflow obstruction. Following obstruction, the weight of the urinary bladder increased more than five-fold within 10 days. Immunohistochemistry with a polyclonal antiserum against the C-terminal sequence of NM-MHC-B revealed very few NM-MHC-B immunoreactive cells in the control urinary bladders. In hypertrophic bladders, the number of NM-MHC-B immunoreactive cells markedly increased. The majority of such cells were found in the interstitium surrounding smooth muscle bundles and also in the subserosal and submucosal layers. Western blot analysis showed that the NM-MHC-B expression was transient; the content of NM-MHC-B immunoreactive material had doubled 10 days after obstruction and then declined towards the control level after 6 weeks. Immunohistochemistry revealed co-localization of NM-MHC-B and vimentin within the same cells. NM-MHC-B did not co-localize with smooth muscle actin, suggesting that the source of NM-MHC-B is not a de-differentiated smooth muscle cell or myofibroblast but a non-muscle cell possibly reacting to tissue distension or stress. The NM-MHC-B-positive cells could have a role in the production of extracellular matrix and growth factors or be involved in modulation of spontaneous contractile activity.},
  author       = {Sjuve, Rolf and Haase, Hannelore and Ekblad, Eva and Malmqvist, Ulf and Morano, Ingo and Arner, Anders},
  issn         = {1432-0878},
  keyword      = {Hypertrophy,Urinary bladder,Non-muscle myosin heavy chain-B (SMemb),Myosin,Rat (Sprague Dawley,female)},
  language     = {eng},
  number       = {2},
  pages        = {271--278},
  publisher    = {Springer},
  series       = {Cell and Tissue Research1974-01-01+01:00},
  title        = {Increased expression of non-muscle myosin heavy chain-B in connective tissue cells of hypertrophic rat urinary bladder},
  url          = {http://dx.doi.org/10.1007/s004410000262},
  volume       = {304},
  year         = {2001},
}