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Differential phosphorylation of Janus kinase 2, Stat5A and Stat5B in response to growth hormone in primary rat adipocytes

Ridderstråle, Martin LU and Groop, Leif LU (2001) In Molecular and Cellular Endocrinology 183(1-2). p.49-54
Abstract
In vitro growth hormone (GH) stimulation of Janus kinase 2 (Jak2) tyrosine phosphorylation and activation has been detected in rat adipocytes where GH exerts both chronic diabetogenic and acute insulin-like effects but not in adipocytes where only chronic diabetogenic effects are exerted. The 95 kDa transcription factor Stat5, which is tyrosine phosphorylated in response to GH in both cases, is here identified as the 5A-isoform. Stat5B was not tyrosine phosphorylated in response to GH in adipocytes but subject to a gel supershift indicating regulation by serine and/or threonine phosphorylation. The differential tyrosine phosphorylation of these proteins suggests involvement of a kinase other than Jak2 in Stat5A activation. However, in... (More)
In vitro growth hormone (GH) stimulation of Janus kinase 2 (Jak2) tyrosine phosphorylation and activation has been detected in rat adipocytes where GH exerts both chronic diabetogenic and acute insulin-like effects but not in adipocytes where only chronic diabetogenic effects are exerted. The 95 kDa transcription factor Stat5, which is tyrosine phosphorylated in response to GH in both cases, is here identified as the 5A-isoform. Stat5B was not tyrosine phosphorylated in response to GH in adipocytes but subject to a gel supershift indicating regulation by serine and/or threonine phosphorylation. The differential tyrosine phosphorylation of these proteins suggests involvement of a kinase other than Jak2 in Stat5A activation. However, in adipocytes where GH exerts both diabetogenic and insulin-like effects, and both Jak2 and Stat5A were activated, their phosphorylation kinetics and downregulation of tyrosine phosphorylation were almost identical. We conclude that Stat5A is important for the diabetogenic actions of GH and that Jak2 still is the most probable candidate kinase for Stat5A in primary adipocytes. (Less)
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Contribution to journal
publication status
published
subject
keywords
Growth hormone (GH), Janus kinase 2 (Jak2), Signal transducer and activator of transcription (Stat), Protein phosphorylation, Lipolysis, Rat adipocyte
in
Molecular and Cellular Endocrinology
volume
183
issue
1-2
pages
49 - 54
publisher
Elsevier
external identifiers
  • pmid:11604224
  • scopus:0035950420
ISSN
1872-8057
DOI
10.1016/S0303-7207(01)00608-6
language
English
LU publication?
yes
id
49294394-2579-4d17-98b2-50fbef269c01 (old id 1122521)
date added to LUP
2016-04-01 12:36:04
date last changed
2022-01-27 07:17:25
@article{49294394-2579-4d17-98b2-50fbef269c01,
  abstract     = {{In vitro growth hormone (GH) stimulation of Janus kinase 2 (Jak2) tyrosine phosphorylation and activation has been detected in rat adipocytes where GH exerts both chronic diabetogenic and acute insulin-like effects but not in adipocytes where only chronic diabetogenic effects are exerted. The 95 kDa transcription factor Stat5, which is tyrosine phosphorylated in response to GH in both cases, is here identified as the 5A-isoform. Stat5B was not tyrosine phosphorylated in response to GH in adipocytes but subject to a gel supershift indicating regulation by serine and/or threonine phosphorylation. The differential tyrosine phosphorylation of these proteins suggests involvement of a kinase other than Jak2 in Stat5A activation. However, in adipocytes where GH exerts both diabetogenic and insulin-like effects, and both Jak2 and Stat5A were activated, their phosphorylation kinetics and downregulation of tyrosine phosphorylation were almost identical. We conclude that Stat5A is important for the diabetogenic actions of GH and that Jak2 still is the most probable candidate kinase for Stat5A in primary adipocytes.}},
  author       = {{Ridderstråle, Martin and Groop, Leif}},
  issn         = {{1872-8057}},
  keywords     = {{Growth hormone (GH); Janus kinase 2 (Jak2); Signal transducer and activator of transcription (Stat); Protein phosphorylation; Lipolysis; Rat adipocyte}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{49--54}},
  publisher    = {{Elsevier}},
  series       = {{Molecular and Cellular Endocrinology}},
  title        = {{Differential phosphorylation of Janus kinase 2, Stat5A and Stat5B in response to growth hormone in primary rat adipocytes}},
  url          = {{http://dx.doi.org/10.1016/S0303-7207(01)00608-6}},
  doi          = {{10.1016/S0303-7207(01)00608-6}},
  volume       = {{183}},
  year         = {{2001}},
}