Advanced

Improved pattern for genome-based screening identifies novel cell wall-attached proteins in gram-positive bacteria

Janulczyk, Robert LU and Rasmussen, Magnus LU (2001) In Infection and Immunity 69(6). p.4019-4026
Abstract
With a large number of sequenced microbial genomes available, tools for identifying groups or classes of proteins have become increasingly important. Here we present an improved pattern for the identification of cell wall-attached proteins (CWPs), a group of proteins with diverse and important functions in gram-positive bacteria. This tripartite pattern is based on analysis of 65 previously described cell wall-attached proteins and takes into account the three principal requirements for cell wall sorting; a sortase target region (LPXTGX), a membrane-spanning region, and a charged stop-transfer tail. In five different genomes of gram-positive bacteria, the tripartite pattern identified a total of 35 putative CWPs, 19 of which were novel.... (More)
With a large number of sequenced microbial genomes available, tools for identifying groups or classes of proteins have become increasingly important. Here we present an improved pattern for the identification of cell wall-attached proteins (CWPs), a group of proteins with diverse and important functions in gram-positive bacteria. This tripartite pattern is based on analysis of 65 previously described cell wall-attached proteins and takes into account the three principal requirements for cell wall sorting; a sortase target region (LPXTGX), a membrane-spanning region, and a charged stop-transfer tail. In five different genomes of gram-positive bacteria, the tripartite pattern identified a total of 35 putative CWPs, 19 of which were novel. The specificity and sensitivity of the tripartite pattern are higher than those of the classical pattern, which is based solely on the sortase target region. Several putative CWPs with atypical sortase target regions were identified. In the complete genome of the important human pathogen Streptococcus pyogenes, the tripartite pattern identified 14 putative CWPs. Seven of the putative S. pyogenes proteins were novel, and two of these were a 5' nucleotidase and a pullulanase. This study represents the first whole-genome screening for CWPs, and we conclude that the tripartite pattern is highly suitable for this purpose. Identification of CWPs using this pattern offers important possibilities in the study of the pathogenesis and physiology of gram-positive bacteria. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Infection and Immunity
volume
69
issue
6
pages
4019 - 4026
publisher
American Society for Microbiology
external identifiers
  • pmid:11349071
  • scopus:0035010116
ISSN
1098-5522
DOI
10.1128/IAI.69.6.4019-4026.2001
language
English
LU publication?
yes
id
93130ccc-db8e-4b31-9ce3-3f44a72fabeb (old id 1122728)
date added to LUP
2008-06-30 16:26:51
date last changed
2018-05-29 12:28:54
@article{93130ccc-db8e-4b31-9ce3-3f44a72fabeb,
  abstract     = {With a large number of sequenced microbial genomes available, tools for identifying groups or classes of proteins have become increasingly important. Here we present an improved pattern for the identification of cell wall-attached proteins (CWPs), a group of proteins with diverse and important functions in gram-positive bacteria. This tripartite pattern is based on analysis of 65 previously described cell wall-attached proteins and takes into account the three principal requirements for cell wall sorting; a sortase target region (LPXTGX), a membrane-spanning region, and a charged stop-transfer tail. In five different genomes of gram-positive bacteria, the tripartite pattern identified a total of 35 putative CWPs, 19 of which were novel. The specificity and sensitivity of the tripartite pattern are higher than those of the classical pattern, which is based solely on the sortase target region. Several putative CWPs with atypical sortase target regions were identified. In the complete genome of the important human pathogen Streptococcus pyogenes, the tripartite pattern identified 14 putative CWPs. Seven of the putative S. pyogenes proteins were novel, and two of these were a 5' nucleotidase and a pullulanase. This study represents the first whole-genome screening for CWPs, and we conclude that the tripartite pattern is highly suitable for this purpose. Identification of CWPs using this pattern offers important possibilities in the study of the pathogenesis and physiology of gram-positive bacteria.},
  author       = {Janulczyk, Robert and Rasmussen, Magnus},
  issn         = {1098-5522},
  language     = {eng},
  number       = {6},
  pages        = {4019--4026},
  publisher    = {American Society for Microbiology},
  series       = {Infection and Immunity},
  title        = {Improved pattern for genome-based screening identifies novel cell wall-attached proteins in gram-positive bacteria},
  url          = {http://dx.doi.org/10.1128/IAI.69.6.4019-4026.2001},
  volume       = {69},
  year         = {2001},
}