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Identification of conditionally expressed genes in Streptococcus pyogenes using RNA fingerprinting

Collin, Mattias LU and Olsén, Arne LU (2001) In FEMS Microbiology Letters 196(2). p.123-127
Abstract
RNA fingerprinting using arbitrarily primed reverse transcription-polymerase chain reaction was employed on isolated RNA from Streptococcus pyogenes bacteria in order to identify genes that were regulated in response to environmental changes. When S. pyogenes was cultured under glucose-rich growth conditions a number of transcriptionally up-regulated products were identified, cloned and sequenced. Using the Streptococcal Genome Sequencing Project database and similarity searches against the GenBank database the corresponding genes encoding enzyme IIB and IIC component of a putative phosphotransferase system were identified. Thus, we show that RNA fingerprinting could be a useful tool to identify unknown genes in S. pyogenes that are... (More)
RNA fingerprinting using arbitrarily primed reverse transcription-polymerase chain reaction was employed on isolated RNA from Streptococcus pyogenes bacteria in order to identify genes that were regulated in response to environmental changes. When S. pyogenes was cultured under glucose-rich growth conditions a number of transcriptionally up-regulated products were identified, cloned and sequenced. Using the Streptococcal Genome Sequencing Project database and similarity searches against the GenBank database the corresponding genes encoding enzyme IIB and IIC component of a putative phosphotransferase system were identified. Thus, we show that RNA fingerprinting could be a useful tool to identify unknown genes in S. pyogenes that are expressed under certain environmental conditions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEMS Microbiology Letters
volume
196
issue
2
pages
123 - 127
publisher
Elsevier
external identifiers
  • pmid:11267767
  • scopus:0035868453
ISSN
1574-6968
DOI
10.1016/S0378-1097(01)00049-0
language
English
LU publication?
yes
id
4dab93e9-7767-48bb-9f1d-205194d92d93 (old id 1123217)
date added to LUP
2008-06-26 09:17:04
date last changed
2018-11-21 20:41:50
@article{4dab93e9-7767-48bb-9f1d-205194d92d93,
  abstract     = {RNA fingerprinting using arbitrarily primed reverse transcription-polymerase chain reaction was employed on isolated RNA from Streptococcus pyogenes bacteria in order to identify genes that were regulated in response to environmental changes. When S. pyogenes was cultured under glucose-rich growth conditions a number of transcriptionally up-regulated products were identified, cloned and sequenced. Using the Streptococcal Genome Sequencing Project database and similarity searches against the GenBank database the corresponding genes encoding enzyme IIB and IIC component of a putative phosphotransferase system were identified. Thus, we show that RNA fingerprinting could be a useful tool to identify unknown genes in S. pyogenes that are expressed under certain environmental conditions.},
  author       = {Collin, Mattias and Olsén, Arne},
  issn         = {1574-6968},
  language     = {eng},
  number       = {2},
  pages        = {123--127},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {Identification of conditionally expressed genes in Streptococcus pyogenes using RNA fingerprinting},
  url          = {http://dx.doi.org/10.1016/S0378-1097(01)00049-0},
  volume       = {196},
  year         = {2001},
}