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EGF and dextran-conjugated EGF induces differential phosphorylation of the EGF receptor

Hägg, Maria; Liljegren, Åsa; Carlsson, Jörgen; Rönnstrand, Lars LU and Lennartsson, Johan (2002) In International Journal of Molecular Medicine 10(5). p.655-659
Abstract
Dextran-conjugated EGF (EGF-dextran) has a potential use for targeted radionuclide therapy of tumors that overexpress the epidermal growth factor receptor (EGFR). There are plans to treat both bladder carcinomas and malignant gliomas with local injections of radiolabeled EGF-dextran since these tumors often express high levels of EGFR. In this report we show that EGF and EGF-dextran differentially activate the EGFR. In the human glioma cell line U-343, activation of the serine/threonine kinases Erk and Akt is identical upon stimulation with EGF or EGF-dextran. However, the effect on phospholipase Cgamma1 (PLCgamma1) phosphorylation differs. In cells stimulated with EGF-dextran, the PLCgamma1 phosphorylation is lower than in cells... (More)
Dextran-conjugated EGF (EGF-dextran) has a potential use for targeted radionuclide therapy of tumors that overexpress the epidermal growth factor receptor (EGFR). There are plans to treat both bladder carcinomas and malignant gliomas with local injections of radiolabeled EGF-dextran since these tumors often express high levels of EGFR. In this report we show that EGF and EGF-dextran differentially activate the EGFR. In the human glioma cell line U-343, activation of the serine/threonine kinases Erk and Akt is identical upon stimulation with EGF or EGF-dextran. However, the effect on phospholipase Cgamma1 (PLCgamma1) phosphorylation differs. In cells stimulated with EGF-dextran, the PLCgamma1 phosphorylation is lower than in cells stimulated with EGF. This observation could be explained by the fact that the PLCgamma1 association sites in the EGFR, tyrosine residues 992 and 1173, were phosphorylated to a lower degree when the receptor was stimulated with EGF-dextran as compared to with EGF. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Molecular Medicine
volume
10
issue
5
pages
655 - 659
publisher
D.A. Spandidos
external identifiers
  • pmid:12373311
  • scopus:0036835585
ISSN
1791-244X
language
English
LU publication?
yes
id
d21d5662-a75d-4e34-8ffc-8b64c6bf9e90 (old id 1123751)
date added to LUP
2008-05-22 13:08:53
date last changed
2017-01-01 07:10:25
@article{d21d5662-a75d-4e34-8ffc-8b64c6bf9e90,
  abstract     = {Dextran-conjugated EGF (EGF-dextran) has a potential use for targeted radionuclide therapy of tumors that overexpress the epidermal growth factor receptor (EGFR). There are plans to treat both bladder carcinomas and malignant gliomas with local injections of radiolabeled EGF-dextran since these tumors often express high levels of EGFR. In this report we show that EGF and EGF-dextran differentially activate the EGFR. In the human glioma cell line U-343, activation of the serine/threonine kinases Erk and Akt is identical upon stimulation with EGF or EGF-dextran. However, the effect on phospholipase Cgamma1 (PLCgamma1) phosphorylation differs. In cells stimulated with EGF-dextran, the PLCgamma1 phosphorylation is lower than in cells stimulated with EGF. This observation could be explained by the fact that the PLCgamma1 association sites in the EGFR, tyrosine residues 992 and 1173, were phosphorylated to a lower degree when the receptor was stimulated with EGF-dextran as compared to with EGF.},
  author       = {Hägg, Maria and Liljegren, Åsa and Carlsson, Jörgen and Rönnstrand, Lars and Lennartsson, Johan},
  issn         = {1791-244X},
  language     = {eng},
  number       = {5},
  pages        = {655--659},
  publisher    = {D.A. Spandidos},
  series       = {International Journal of Molecular Medicine},
  title        = {EGF and dextran-conjugated EGF induces differential phosphorylation of the EGF receptor},
  volume       = {10},
  year         = {2002},
}