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Timing the doxycycline yields different patterns of genomic recombination in brain neurons with a new inducible Cre transgene.

Lindeberg, J; Mattsson, Ragnar LU and Ebendal, T (2002) In Journal of Neuroscience Research 68(2). p.248-253
Abstract
We have developed a transgenic mouse expressing the Cre recombinase under control of a tetracycline-responsive promoter. Using a CamKIIalpha-driven tTA transgenic strain and a lacZ reporter mouse, we obtained the expected neuronal pattern of recombination in the olfactory lobe, cortex, striatum, hippocampus and Purkinje cells. Moreover, recombination can be completely abolished by feeding the mice doxycycline in their drinking water. We also show that it is possible to get a different pattern of recombination by changing the timing of the doxycycline-mediated shutdown of Cre expression. By starting the doxycycline treatment at birth, we restrict recombination to striatum only. This approach should be applicable to other inducible... (More)
We have developed a transgenic mouse expressing the Cre recombinase under control of a tetracycline-responsive promoter. Using a CamKIIalpha-driven tTA transgenic strain and a lacZ reporter mouse, we obtained the expected neuronal pattern of recombination in the olfactory lobe, cortex, striatum, hippocampus and Purkinje cells. Moreover, recombination can be completely abolished by feeding the mice doxycycline in their drinking water. We also show that it is possible to get a different pattern of recombination by changing the timing of the doxycycline-mediated shutdown of Cre expression. By starting the doxycycline treatment at birth, we restrict recombination to striatum only. This approach should be applicable to other inducible transgenic strains, thus increasing the number of available tissue-specific patterns for conditional knockouts. Also, our tetO-Cre transgene can be combined with any of the increasing number of tetracycline transactivator transgenic strains to direct specifically inducible genomic recombination to several areas of the brain. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Neuroscience Research
volume
68
issue
2
pages
248 - 253
publisher
John Wiley & Sons
external identifiers
  • scopus:0036217859
ISSN
1097-4547
DOI
10.1002/jnr.10213
language
English
LU publication?
no
id
364a8667-0c0b-44e0-9e3a-39d332a1c63e (old id 1124977)
date added to LUP
2008-05-26 15:28:17
date last changed
2017-01-01 06:42:06
@article{364a8667-0c0b-44e0-9e3a-39d332a1c63e,
  abstract     = {We have developed a transgenic mouse expressing the Cre recombinase under control of a tetracycline-responsive promoter. Using a CamKIIalpha-driven tTA transgenic strain and a lacZ reporter mouse, we obtained the expected neuronal pattern of recombination in the olfactory lobe, cortex, striatum, hippocampus and Purkinje cells. Moreover, recombination can be completely abolished by feeding the mice doxycycline in their drinking water. We also show that it is possible to get a different pattern of recombination by changing the timing of the doxycycline-mediated shutdown of Cre expression. By starting the doxycycline treatment at birth, we restrict recombination to striatum only. This approach should be applicable to other inducible transgenic strains, thus increasing the number of available tissue-specific patterns for conditional knockouts. Also, our tetO-Cre transgene can be combined with any of the increasing number of tetracycline transactivator transgenic strains to direct specifically inducible genomic recombination to several areas of the brain.},
  author       = {Lindeberg, J and Mattsson, Ragnar and Ebendal, T},
  issn         = {1097-4547},
  language     = {eng},
  number       = {2},
  pages        = {248--253},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Neuroscience Research},
  title        = {Timing the doxycycline yields different patterns of genomic recombination in brain neurons with a new inducible Cre transgene.},
  url          = {http://dx.doi.org/10.1002/jnr.10213},
  volume       = {68},
  year         = {2002},
}