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Genetic dissection of lymphopenia from autoimmunity by introgression of mutated Ian5 gene onto the F344 rat.

Moralejo, Daniel H ; Park, Hyunhee A ; Speros, Sara J ; MacMurray, Armand J ; Kwitek, Anne E ; Jacob, Howard J ; Lander, Eric S and Lernmark, Åke LU orcid (2003) In Journal of Autoimmunity 21(4). p.315-324
Abstract
Peripheral T cell lymphopenia (lyp) in the BioBreeding (BB) rat is linked to a frameshift mutation in Ian5, a member of the Immune Associated Nucleotide (Ian) gene family on rat chromosome 4. This lymphopenia leads to type 1 (insulin-dependent) diabetes mellitus (T1DM) at rates up to 100% when combined with the BB rat MHC RT1 u/u genotype. In order, to better study the lymphopenia phenotype without possible confounding effects of diabetes or other autoimmune disease, we generated congenic F344.lyp rats by introgression of lyp on diabetes-resistant MHC RT1 lv1/lv1 F344 rats. Analysis of thymic CD4 and CD8 T lymphocytes revealed no difference in the percentage of CD4(-)CD8(+)and CD4(+)CD8(-)subsets in lyp/lyp compared to +/+ F344 rats. The... (More)
Peripheral T cell lymphopenia (lyp) in the BioBreeding (BB) rat is linked to a frameshift mutation in Ian5, a member of the Immune Associated Nucleotide (Ian) gene family on rat chromosome 4. This lymphopenia leads to type 1 (insulin-dependent) diabetes mellitus (T1DM) at rates up to 100% when combined with the BB rat MHC RT1 u/u genotype. In order, to better study the lymphopenia phenotype without possible confounding effects of diabetes or other autoimmune disease, we generated congenic F344.lyp rats by introgression of lyp on diabetes-resistant MHC RT1 lv1/lv1 F344 rats. Analysis of thymic CD4 and CD8 T lymphocytes revealed no difference in the percentage of CD4(-)CD8(+)and CD4(+)CD8(-)subsets in lyp/lyp compared to +/+ F344 rats. The same subsets was however dramatically reduced in blood (P=0.005), spleen (P=0.019) and mesenteric lymph nodes (MLN) (P<0.0001). Compared to F344 +/+ rats double positive CD4(+)CD8(+)T cells were increased only in lyp/lyp spleen (P=0.034) while double n (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Autoimmunity
volume
21
issue
4
pages
315 - 324
publisher
Elsevier
external identifiers
  • scopus:0242659141
ISSN
0896-8411
DOI
10.1016/S0896-8411(03)00138-0
language
English
LU publication?
yes
id
efa2e295-df21-4565-96e0-d7c2876aed76 (old id 1127389)
date added to LUP
2016-04-01 11:41:31
date last changed
2022-03-05 05:03:01
@article{efa2e295-df21-4565-96e0-d7c2876aed76,
  abstract     = {{Peripheral T cell lymphopenia (lyp) in the BioBreeding (BB) rat is linked to a frameshift mutation in Ian5, a member of the Immune Associated Nucleotide (Ian) gene family on rat chromosome 4. This lymphopenia leads to type 1 (insulin-dependent) diabetes mellitus (T1DM) at rates up to 100% when combined with the BB rat MHC RT1 u/u genotype. In order, to better study the lymphopenia phenotype without possible confounding effects of diabetes or other autoimmune disease, we generated congenic F344.lyp rats by introgression of lyp on diabetes-resistant MHC RT1 lv1/lv1 F344 rats. Analysis of thymic CD4 and CD8 T lymphocytes revealed no difference in the percentage of CD4(-)CD8(+)and CD4(+)CD8(-)subsets in lyp/lyp compared to +/+ F344 rats. The same subsets was however dramatically reduced in blood (P=0.005), spleen (P=0.019) and mesenteric lymph nodes (MLN) (P&lt;0.0001). Compared to F344 +/+ rats double positive CD4(+)CD8(+)T cells were increased only in lyp/lyp spleen (P=0.034) while double n}},
  author       = {{Moralejo, Daniel H and Park, Hyunhee A and Speros, Sara J and MacMurray, Armand J and Kwitek, Anne E and Jacob, Howard J and Lander, Eric S and Lernmark, Åke}},
  issn         = {{0896-8411}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{315--324}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Autoimmunity}},
  title        = {{Genetic dissection of lymphopenia from autoimmunity by introgression of mutated Ian5 gene onto the F344 rat.}},
  url          = {{http://dx.doi.org/10.1016/S0896-8411(03)00138-0}},
  doi          = {{10.1016/S0896-8411(03)00138-0}},
  volume       = {{21}},
  year         = {{2003}},
}