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Genetic dissection of lymphopenia from autoimmunity by introgression of mutated Ian5 gene onto the F344 rat.

Moralejo, Daniel H; Park, Hyunhee A; Speros, Sara J; MacMurray, Armand J; Kwitek, Anne E; Jacob, Howard J; Lander, Eric S and Lernmark, Åke LU (2003) In Journal of Autoimmunity 21(4). p.315-324
Abstract
Peripheral T cell lymphopenia (lyp) in the BioBreeding (BB) rat is linked to a frameshift mutation in Ian5, a member of the Immune Associated Nucleotide (Ian) gene family on rat chromosome 4. This lymphopenia leads to type 1 (insulin-dependent) diabetes mellitus (T1DM) at rates up to 100% when combined with the BB rat MHC RT1 u/u genotype. In order, to better study the lymphopenia phenotype without possible confounding effects of diabetes or other autoimmune disease, we generated congenic F344.lyp rats by introgression of lyp on diabetes-resistant MHC RT1 lv1/lv1 F344 rats. Analysis of thymic CD4 and CD8 T lymphocytes revealed no difference in the percentage of CD4(-)CD8(+)and CD4(+)CD8(-)subsets in lyp/lyp compared to +/+ F344 rats. The... (More)
Peripheral T cell lymphopenia (lyp) in the BioBreeding (BB) rat is linked to a frameshift mutation in Ian5, a member of the Immune Associated Nucleotide (Ian) gene family on rat chromosome 4. This lymphopenia leads to type 1 (insulin-dependent) diabetes mellitus (T1DM) at rates up to 100% when combined with the BB rat MHC RT1 u/u genotype. In order, to better study the lymphopenia phenotype without possible confounding effects of diabetes or other autoimmune disease, we generated congenic F344.lyp rats by introgression of lyp on diabetes-resistant MHC RT1 lv1/lv1 F344 rats. Analysis of thymic CD4 and CD8 T lymphocytes revealed no difference in the percentage of CD4(-)CD8(+)and CD4(+)CD8(-)subsets in lyp/lyp compared to +/+ F344 rats. The same subsets was however dramatically reduced in blood (P=0.005), spleen (P=0.019) and mesenteric lymph nodes (MLN) (P<0.0001). Compared to F344 +/+ rats double positive CD4(+)CD8(+)T cells were increased only in lyp/lyp spleen (P=0.034) while double n (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Autoimmunity
volume
21
issue
4
pages
315 - 324
publisher
Elsevier
external identifiers
  • scopus:0242659141
ISSN
0896-8411
DOI
10.1016/S0896-8411(03)00138-0
language
English
LU publication?
yes
id
efa2e295-df21-4565-96e0-d7c2876aed76 (old id 1127389)
date added to LUP
2008-06-09 12:00:42
date last changed
2018-10-03 10:45:37
@article{efa2e295-df21-4565-96e0-d7c2876aed76,
  abstract     = {Peripheral T cell lymphopenia (lyp) in the BioBreeding (BB) rat is linked to a frameshift mutation in Ian5, a member of the Immune Associated Nucleotide (Ian) gene family on rat chromosome 4. This lymphopenia leads to type 1 (insulin-dependent) diabetes mellitus (T1DM) at rates up to 100% when combined with the BB rat MHC RT1 u/u genotype. In order, to better study the lymphopenia phenotype without possible confounding effects of diabetes or other autoimmune disease, we generated congenic F344.lyp rats by introgression of lyp on diabetes-resistant MHC RT1 lv1/lv1 F344 rats. Analysis of thymic CD4 and CD8 T lymphocytes revealed no difference in the percentage of CD4(-)CD8(+)and CD4(+)CD8(-)subsets in lyp/lyp compared to +/+ F344 rats. The same subsets was however dramatically reduced in blood (P=0.005), spleen (P=0.019) and mesenteric lymph nodes (MLN) (P&lt;0.0001). Compared to F344 +/+ rats double positive CD4(+)CD8(+)T cells were increased only in lyp/lyp spleen (P=0.034) while double n},
  author       = {Moralejo, Daniel H and Park, Hyunhee A and Speros, Sara J and MacMurray, Armand J and Kwitek, Anne E and Jacob, Howard J and Lander, Eric S and Lernmark, Åke},
  issn         = {0896-8411},
  language     = {eng},
  number       = {4},
  pages        = {315--324},
  publisher    = {Elsevier},
  series       = {Journal of Autoimmunity},
  title        = {Genetic dissection of lymphopenia from autoimmunity by introgression of mutated Ian5 gene onto the F344 rat.},
  url          = {http://dx.doi.org/10.1016/S0896-8411(03)00138-0},
  volume       = {21},
  year         = {2003},
}