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Osteoblast recruitment from stem cells does not decrease by age at late adulthood

Leskela, Hannu-Ville; Risteli, Juha; Niskanen, Salla; Koivunen, Jussi; Ivaska, Kaisa LU and Lehenkari, Petri (2003) In Biochemical and Biophysical Research Communications 311(4). p.1008-1013
Abstract
This study was aimed to characterize the ability of human bone marrow mesenchymal stem cells (MSC) to differentiate into osteoblasts in vitro. Twenty-three women and 20 men at late adulthood (52-92 years of age) were selected for the study. MSCs were isolated and cultured in vitro and alkaline phosphatase (ALP) activity, secretion of amino-terminal propeptide of type I procollagen (PINP), type III procollagen (PIIINP) and osteocalcin were analyzed. Matrix mineralization was analyzed by the von Kossa staining and by calcium quantification. We found that the ALP and PINP levels compared with control increased to 2.8- and 2.9-fold, respectively, when cells were cultured for three weeks. ALP activity, PINP and calcium deposition in response to... (More)
This study was aimed to characterize the ability of human bone marrow mesenchymal stem cells (MSC) to differentiate into osteoblasts in vitro. Twenty-three women and 20 men at late adulthood (52-92 years of age) were selected for the study. MSCs were isolated and cultured in vitro and alkaline phosphatase (ALP) activity, secretion of amino-terminal propeptide of type I procollagen (PINP), type III procollagen (PIIINP) and osteocalcin were analyzed. Matrix mineralization was analyzed by the von Kossa staining and by calcium quantification. We found that the ALP and PINP levels compared with control increased to 2.8- and 2.9-fold, respectively, when cells were cultured for three weeks. ALP activity, PINP and calcium deposition in response to dexamethasone treatment increased by age in women and was unchanged in men. Overall our data suggests that the osteogenic potential of MSCs does not decrease by age in either women or men at late adulthood. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical and Biophysical Research Communications
volume
311
issue
4
pages
1008 - 1013
publisher
Elsevier
external identifiers
  • pmid:14623282
  • scopus:0242437917
ISSN
1090-2104
DOI
10.1016/j.bbrc.2003.10.095
language
English
LU publication?
yes
id
42fffe76-0ee1-429c-becd-7fb12f7e4d34 (old id 1127513)
date added to LUP
2008-06-09 08:27:38
date last changed
2017-08-06 04:22:47
@article{42fffe76-0ee1-429c-becd-7fb12f7e4d34,
  abstract     = {This study was aimed to characterize the ability of human bone marrow mesenchymal stem cells (MSC) to differentiate into osteoblasts in vitro. Twenty-three women and 20 men at late adulthood (52-92 years of age) were selected for the study. MSCs were isolated and cultured in vitro and alkaline phosphatase (ALP) activity, secretion of amino-terminal propeptide of type I procollagen (PINP), type III procollagen (PIIINP) and osteocalcin were analyzed. Matrix mineralization was analyzed by the von Kossa staining and by calcium quantification. We found that the ALP and PINP levels compared with control increased to 2.8- and 2.9-fold, respectively, when cells were cultured for three weeks. ALP activity, PINP and calcium deposition in response to dexamethasone treatment increased by age in women and was unchanged in men. Overall our data suggests that the osteogenic potential of MSCs does not decrease by age in either women or men at late adulthood.},
  author       = {Leskela, Hannu-Ville and Risteli, Juha and Niskanen, Salla and Koivunen, Jussi and Ivaska, Kaisa and Lehenkari, Petri},
  issn         = {1090-2104},
  language     = {eng},
  number       = {4},
  pages        = {1008--1013},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Osteoblast recruitment from stem cells does not decrease by age at late adulthood},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2003.10.095},
  volume       = {311},
  year         = {2003},
}