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Studies on correlation of antimutagenic and antiproliferative activities of Juglans regia L

Kaur, Kamaljit; Michael, Husheem; Arora, Saroj; Härkönen, Pirkko LU and Kumar, Subodh (2003) In Journal of Environmental Pathology and Toxicology 22(1). p.59-67
Abstract
We investigated the effect of water and acetone extract of Juglans regia L. to evaluate its antimutagenic and antiproliferative activities. The antimutagenic study using TA98 and TA100 tester strains of Salmonella revealed the water and acetone extracts to be more effective than the benzene and chloroform extracts in inhibiting the revertants induced by 2-aminoflourene (2AF) in TA100 tester strains. The most effective extracts in the Ames assay were further evaluated using the Lucifer luciferase assay and in time course studies for antiproliferative activities using the Hoechst staining to observe apoptotic cell deaths. The acetone extract showed a correlation of antimutagenic activities in the Ames assay with its antiproliferative effect... (More)
We investigated the effect of water and acetone extract of Juglans regia L. to evaluate its antimutagenic and antiproliferative activities. The antimutagenic study using TA98 and TA100 tester strains of Salmonella revealed the water and acetone extracts to be more effective than the benzene and chloroform extracts in inhibiting the revertants induced by 2-aminoflourene (2AF) in TA100 tester strains. The most effective extracts in the Ames assay were further evaluated using the Lucifer luciferase assay and in time course studies for antiproliferative activities using the Hoechst staining to observe apoptotic cell deaths. The acetone extract showed a correlation of antimutagenic activities in the Ames assay with its antiproliferative effect in different cell lines, while the water extract exerted its effect distinctly in each cell line. Further studies are still needed to evaluate the cytotoxicity in experiments carried out in vivo. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Environmental Pathology and Toxicology
volume
22
issue
1
pages
59 - 67
publisher
Begell House
external identifiers
  • scopus:0037240428
ISSN
2162-6537
DOI
10.1615/JEnvPathToxOncol.v22.i1.60
language
English
LU publication?
yes
id
411d6697-7658-44fc-ab7c-a18379721fa1 (old id 1127606)
date added to LUP
2008-06-05 14:11:52
date last changed
2018-10-03 11:43:08
@article{411d6697-7658-44fc-ab7c-a18379721fa1,
  abstract     = {We investigated the effect of water and acetone extract of Juglans regia L. to evaluate its antimutagenic and antiproliferative activities. The antimutagenic study using TA98 and TA100 tester strains of Salmonella revealed the water and acetone extracts to be more effective than the benzene and chloroform extracts in inhibiting the revertants induced by 2-aminoflourene (2AF) in TA100 tester strains. The most effective extracts in the Ames assay were further evaluated using the Lucifer luciferase assay and in time course studies for antiproliferative activities using the Hoechst staining to observe apoptotic cell deaths. The acetone extract showed a correlation of antimutagenic activities in the Ames assay with its antiproliferative effect in different cell lines, while the water extract exerted its effect distinctly in each cell line. Further studies are still needed to evaluate the cytotoxicity in experiments carried out in vivo.},
  author       = {Kaur, Kamaljit and Michael, Husheem and Arora, Saroj and Härkönen, Pirkko and Kumar, Subodh},
  issn         = {2162-6537},
  language     = {eng},
  number       = {1},
  pages        = {59--67},
  publisher    = {Begell House},
  series       = {Journal of Environmental Pathology and Toxicology},
  title        = {Studies on correlation of antimutagenic and antiproliferative activities of Juglans regia L},
  url          = {http://dx.doi.org/10.1615/JEnvPathToxOncol.v22.i1.60},
  volume       = {22},
  year         = {2003},
}