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Exprese MMP-26 v endometrialnich explantech pod vlivem estradiolu a progesteronu

Pilka, Radovan LU ; Kudela, M; Hansson, Stefan LU and Casslén, Bertil LU (2004) In Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne 69(6). p.467-471
Abstract
OBJECTIVE: To examine possible estrogen dependent endometrial expression of MMP-26 in vitro. DESIGN: Experimental study. SETTING: Department of Obstetrics and Gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden. METHODS: We studied MMP-26 mRNA in 14 normal endometrial samples obtained from the proliferative phase of the menstrual cycle. Samples were cultured for five days either with estradiol alone or in combination with progesterone. Samples cultured with ethanol represented control groups. MMP-26 mRNA expression was examined in frozen samples using in situ hybridization. Immunohistochemistry was used to study the... (More)
OBJECTIVE: To examine possible estrogen dependent endometrial expression of MMP-26 in vitro. DESIGN: Experimental study. SETTING: Department of Obstetrics and Gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden. METHODS: We studied MMP-26 mRNA in 14 normal endometrial samples obtained from the proliferative phase of the menstrual cycle. Samples were cultured for five days either with estradiol alone or in combination with progesterone. Samples cultured with ethanol represented control groups. MMP-26 mRNA expression was examined in frozen samples using in situ hybridization. Immunohistochemistry was used to study the presence of estrogen and progesterone receptors in endometrial explants. RESULTS: MMP-26 mRNA expression was highest in fresh (non cultured) samples. Signal intensity decreased during the first two days of culture and was negligable in the following days. Nuclear intensity for estrogen and progesterone receptor was high after five days of culture. CONCLUSION: We did not find MMP-26 mRNA in vitro expression to be directly estrogen dependent. (Less)
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author
organization
alternative title
MMP-26 expression in endometrial explants treated with estradiol and progesterone
publishing date
type
Contribution to journal
publication status
published
subject
in
Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
volume
69
issue
6
pages
467 - 471
publisher
Ceska Lekarska Spolecnost J. E. Purkyne
external identifiers
  • pmid:15633416
  • scopus:9644255836
ISSN
1210-7832
language
Other
LU publication?
yes
id
71a86fd8-083d-41b0-98c5-44f23e660d7b (old id 1129633)
date added to LUP
2008-06-18 09:34:38
date last changed
2017-01-01 06:39:24
@article{71a86fd8-083d-41b0-98c5-44f23e660d7b,
  abstract     = {OBJECTIVE: To examine possible estrogen dependent endometrial expression of MMP-26 in vitro. DESIGN: Experimental study. SETTING: Department of Obstetrics and Gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden. METHODS: We studied MMP-26 mRNA in 14 normal endometrial samples obtained from the proliferative phase of the menstrual cycle. Samples were cultured for five days either with estradiol alone or in combination with progesterone. Samples cultured with ethanol represented control groups. MMP-26 mRNA expression was examined in frozen samples using in situ hybridization. Immunohistochemistry was used to study the presence of estrogen and progesterone receptors in endometrial explants. RESULTS: MMP-26 mRNA expression was highest in fresh (non cultured) samples. Signal intensity decreased during the first two days of culture and was negligable in the following days. Nuclear intensity for estrogen and progesterone receptor was high after five days of culture. CONCLUSION: We did not find MMP-26 mRNA in vitro expression to be directly estrogen dependent.},
  author       = {Pilka, Radovan and Kudela, M and Hansson, Stefan and Casslén, Bertil},
  issn         = {1210-7832},
  language     = {mis},
  number       = {6},
  pages        = {467--471},
  publisher    = {Ceska Lekarska Spolecnost J. E. Purkyne},
  series       = {Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne},
  title        = {Exprese MMP-26 v endometrialnich explantech pod vlivem estradiolu a progesteronu},
  volume       = {69},
  year         = {2004},
}