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BRCA1-mediated repression of select X chromosome genes

Jazaeri, Amir A; Chandramouli, Gadisetti VR; Aprelikova, Olga; Nuber, Ulrike LU ; Sotiriou, Christos; Liu, Edison T; Ropers, H Hilger; Yee, Cindy J; Boyd, Jeff and Barrett, J Carl (2004) In Journal of Translational Medicine 2(1).
Abstract
Recently BRCA1 has been implicated in the regulation of gene expression from the X chromosome. In this study the influence of BRCA1 on expression of X chromosome genes was investigated. Complementary DNA microarrays were used to compare the expression levels of X chromosome genes in 18 BRCA1-associated ovarian cancers to those of the 13 "BRCA1-like" and 14 "BRCA2-like" sporadic tumors (as defined by previously reported expression profiling). Significance was determined using parametric statistics with P < 0.005 as a cutoff. Forty of 178 total X-chromosome transcripts were differentially expressed between the BRCA1-associated tumors and sporadic cancers with a BRCA2-like molecular profile. Thirty of these 40 genes showed higher mean... (More)
Recently BRCA1 has been implicated in the regulation of gene expression from the X chromosome. In this study the influence of BRCA1 on expression of X chromosome genes was investigated. Complementary DNA microarrays were used to compare the expression levels of X chromosome genes in 18 BRCA1-associated ovarian cancers to those of the 13 "BRCA1-like" and 14 "BRCA2-like" sporadic tumors (as defined by previously reported expression profiling). Significance was determined using parametric statistics with P < 0.005 as a cutoff. Forty of 178 total X-chromosome transcripts were differentially expressed between the BRCA1-associated tumors and sporadic cancers with a BRCA2-like molecular profile. Thirty of these 40 genes showed higher mean expression in the BRCA1-associated samples including all 11 transcripts that mapped to Xp11. In contrast, four of 178 total X chromosome transcripts showed significant differential expression between BRCA1-associated and sporadic tumors with a BRCA1-like molecular profile. All four mapped to Xp11 and showed higher mean expression in BRCA1-associated tumors. Re-expression of BRCA1 in HCC1937 BRCA1-deficient breast cancer cell resulted in the repression of 21 transcripts. Eleven of the 21 (54.5%) transcripts mapped to Xp11. However, there was no significant overlap between these Xp11 genes and those found to be differentially expressed between BRCA1-associated and sporadic ovarian cancer samples. These results demonstrate that BRCA1 mediates the repression of several X chromosome genes, many of which map to the Xp11 locus. (Less)
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author
publishing date
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Contribution to journal
publication status
published
subject
in
Journal of Translational Medicine
volume
2
issue
1
publisher
BioMed Central
external identifiers
  • PMID:15383145
  • Scopus:11044236365
ISSN
1479-5876
DOI
10.1186/1479-5876-2-32
language
English
LU publication?
no
id
a42f31fc-50e9-494b-a1e8-5f6417ae4db9 (old id 1131012)
date added to LUP
2008-06-19 13:17:26
date last changed
2017-01-01 07:53:25
@article{a42f31fc-50e9-494b-a1e8-5f6417ae4db9,
  abstract     = {Recently BRCA1 has been implicated in the regulation of gene expression from the X chromosome. In this study the influence of BRCA1 on expression of X chromosome genes was investigated. Complementary DNA microarrays were used to compare the expression levels of X chromosome genes in 18 BRCA1-associated ovarian cancers to those of the 13 "BRCA1-like" and 14 "BRCA2-like" sporadic tumors (as defined by previously reported expression profiling). Significance was determined using parametric statistics with P &lt; 0.005 as a cutoff. Forty of 178 total X-chromosome transcripts were differentially expressed between the BRCA1-associated tumors and sporadic cancers with a BRCA2-like molecular profile. Thirty of these 40 genes showed higher mean expression in the BRCA1-associated samples including all 11 transcripts that mapped to Xp11. In contrast, four of 178 total X chromosome transcripts showed significant differential expression between BRCA1-associated and sporadic tumors with a BRCA1-like molecular profile. All four mapped to Xp11 and showed higher mean expression in BRCA1-associated tumors. Re-expression of BRCA1 in HCC1937 BRCA1-deficient breast cancer cell resulted in the repression of 21 transcripts. Eleven of the 21 (54.5%) transcripts mapped to Xp11. However, there was no significant overlap between these Xp11 genes and those found to be differentially expressed between BRCA1-associated and sporadic ovarian cancer samples. These results demonstrate that BRCA1 mediates the repression of several X chromosome genes, many of which map to the Xp11 locus.},
  articleno    = {32},
  author       = {Jazaeri, Amir A and Chandramouli, Gadisetti VR and Aprelikova, Olga and Nuber, Ulrike and Sotiriou, Christos and Liu, Edison T and Ropers, H Hilger and Yee, Cindy J and Boyd, Jeff and Barrett, J Carl},
  issn         = {1479-5876},
  language     = {eng},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {Journal of Translational Medicine},
  title        = {BRCA1-mediated repression of select X chromosome genes},
  url          = {http://dx.doi.org/10.1186/1479-5876-2-32},
  volume       = {2},
  year         = {2004},
}