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Plasma bikunin: half-life and tissue uptake

Kaczmarczyk, Aneta; Blom, Anna LU ; Alston-Smith, James; Sjoquist, Mats and Fries, Erik (2005) In Molecular and Cellular Biochemistry 271(1-2). p.61-67
Abstract
Bikunin is a chondroitin sulfate-containing plasma protein synthesized in the liver. In vitro, it has been shown to inhibit proteases and to have additional activities, but its biological function is still unclear. Here we have studied the dynamics of plasma bikunin in rats and mice. A half-life of 7 +/- 2 min was obtained from the time course of the decrease of the plasma level of bikunin following hepatectomy. Clearance experiments with intravenously injected radiolabeled bikunin with or without the chondroitin sulfate chain showed that the polysaccharide had little influence on the elimination rate of the protein. The uptake of bikunin by different tissues was studied using bikunin labeled with the residualizing agent 125I-tyramine... (More)
Bikunin is a chondroitin sulfate-containing plasma protein synthesized in the liver. In vitro, it has been shown to inhibit proteases and to have additional activities, but its biological function is still unclear. Here we have studied the dynamics of plasma bikunin in rats and mice. A half-life of 7 +/- 2 min was obtained from the time course of the decrease of the plasma level of bikunin following hepatectomy. Clearance experiments with intravenously injected radiolabeled bikunin with or without the chondroitin sulfate chain showed that the polysaccharide had little influence on the elimination rate of the protein. The uptake of bikunin by different tissues was studied using bikunin labeled with the residualizing agent 125I-tyramine cellobiose; 60 min after intravenous injection, 49% of the radioactivity was recovered in the kidneys and 6-11% in the liver, bones, skin, intestine and skeletal muscle. The uptake in the liver was analyzed by intravenous injection of radiolabeled bikunin followed by collagenase perfusion and dispersion of the liver cells. These experiments indicated that bikunin is first trapped extracellularly within the liver before being internalized by the cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bikunin, chondroitin sulfate, half-life, hepatectomy, liver perfusion, tissue distribution, tyramine cellobiose
in
Molecular and Cellular Biochemistry
volume
271
issue
1-2
pages
61 - 67
publisher
Springer
external identifiers
  • pmid:15881656
  • scopus:17244379212
ISSN
0300-8177
DOI
10.1007/s11010-005-5282-3
language
English
LU publication?
yes
id
aefb57c2-de36-4ec8-8241-bbb7315ce30b (old id 1132458)
date added to LUP
2008-06-27 13:49:26
date last changed
2017-01-15 04:09:33
@article{aefb57c2-de36-4ec8-8241-bbb7315ce30b,
  abstract     = {Bikunin is a chondroitin sulfate-containing plasma protein synthesized in the liver. In vitro, it has been shown to inhibit proteases and to have additional activities, but its biological function is still unclear. Here we have studied the dynamics of plasma bikunin in rats and mice. A half-life of 7 +/- 2 min was obtained from the time course of the decrease of the plasma level of bikunin following hepatectomy. Clearance experiments with intravenously injected radiolabeled bikunin with or without the chondroitin sulfate chain showed that the polysaccharide had little influence on the elimination rate of the protein. The uptake of bikunin by different tissues was studied using bikunin labeled with the residualizing agent 125I-tyramine cellobiose; 60 min after intravenous injection, 49% of the radioactivity was recovered in the kidneys and 6-11% in the liver, bones, skin, intestine and skeletal muscle. The uptake in the liver was analyzed by intravenous injection of radiolabeled bikunin followed by collagenase perfusion and dispersion of the liver cells. These experiments indicated that bikunin is first trapped extracellularly within the liver before being internalized by the cells.},
  author       = {Kaczmarczyk, Aneta and Blom, Anna and Alston-Smith, James and Sjoquist, Mats and Fries, Erik},
  issn         = {0300-8177},
  keyword      = {bikunin,chondroitin sulfate,half-life,hepatectomy,liver perfusion,tissue distribution,tyramine cellobiose},
  language     = {eng},
  number       = {1-2},
  pages        = {61--67},
  publisher    = {Springer},
  series       = {Molecular and Cellular Biochemistry},
  title        = {Plasma bikunin: half-life and tissue uptake},
  url          = {http://dx.doi.org/10.1007/s11010-005-5282-3},
  volume       = {271},
  year         = {2005},
}