Up-regulation of Toll-like receptors 2, 3 and 4 in allergic rhinitis
(2005) In Respiratory Research 6(100).- Abstract
- BACKGROUND: Toll-like receptors enable the host to recognize a large number of pathogen-associated molecular patterns such as bacterial lipopolysaccharide, viral RNA, CpG-containing DNA and flagellin. Toll-like receptors have also been shown to play a pivotal role in both innate and adaptive immune responses. The role of Toll-like receptors as a primary part of our microbe defense system has been shown in several studies, but their possible function as mediators in allergy and asthma remains to be established. The present study was designed to examine the expression of Toll-like receptors 2, 3 and 4 in the nasal mucosa of patients with intermittent allergic rhinitis, focusing on changes induced by exposure to pollen. METHODS: 27 healthy... (More)
- BACKGROUND: Toll-like receptors enable the host to recognize a large number of pathogen-associated molecular patterns such as bacterial lipopolysaccharide, viral RNA, CpG-containing DNA and flagellin. Toll-like receptors have also been shown to play a pivotal role in both innate and adaptive immune responses. The role of Toll-like receptors as a primary part of our microbe defense system has been shown in several studies, but their possible function as mediators in allergy and asthma remains to be established. The present study was designed to examine the expression of Toll-like receptors 2, 3 and 4 in the nasal mucosa of patients with intermittent allergic rhinitis, focusing on changes induced by exposure to pollen. METHODS: 27 healthy controls and 42 patients with seasonal allergic rhinitis volunteered for the study. Nasal biopsies were obtained before and during pollen season as well as before and after allergen challenge. The seasonal material was used for mRNA quantification of Toll-like receptors 2, 3 and 4 with real-time polymerase chain reaction, whereas specimens achieved in conjunction with allergen challenge were used for immunohistochemical localization and quantification of corresponding proteins. RESULTS: mRNA and protein representing Toll-like receptors 2, 3 and 4 could be demonstrated in all specimens. An increase in protein expression for all three receptors could be seen following allergen challenge, whereas a significant increase of mRNA only could be obtained for Toll-like receptor 3 during pollen season. CONCLUSION: The up-regulation of Toll-like receptors 2, 3 and 4 in the nasal mucosa of patients with symptomatic allergic rhinitis supports the idea of a role for Toll-like receptors in allergic airway inflammation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1132625
- author
- Fransson, Mattias LU ; Adner, Mikael LU ; Erjefält, Jonas LU ; Jansson, Lennart ; Uddman, Rolf LU and Cardell, Lars-Olaf LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Respiratory Research
- volume
- 6
- issue
- 100
- publisher
- BioMed Central (BMC)
- external identifiers
-
- pmid:16146574
- wos:000232280600002
- scopus:26244461464
- pmid:16146574
- ISSN
- 1465-9921
- DOI
- 10.1186/1465-9921-6-100
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Reconstructive Surgery (013240300), Clinical and Experimental Allergy Research (013243510), Airway Inflammation and Immunology (013212038)
- id
- 945ef6cc-44d0-4068-a2f3-44b6e97e0f41 (old id 1132625)
- date added to LUP
- 2016-04-01 11:53:49
- date last changed
- 2022-04-20 23:25:21
@article{945ef6cc-44d0-4068-a2f3-44b6e97e0f41,
abstract = {{BACKGROUND: Toll-like receptors enable the host to recognize a large number of pathogen-associated molecular patterns such as bacterial lipopolysaccharide, viral RNA, CpG-containing DNA and flagellin. Toll-like receptors have also been shown to play a pivotal role in both innate and adaptive immune responses. The role of Toll-like receptors as a primary part of our microbe defense system has been shown in several studies, but their possible function as mediators in allergy and asthma remains to be established. The present study was designed to examine the expression of Toll-like receptors 2, 3 and 4 in the nasal mucosa of patients with intermittent allergic rhinitis, focusing on changes induced by exposure to pollen. METHODS: 27 healthy controls and 42 patients with seasonal allergic rhinitis volunteered for the study. Nasal biopsies were obtained before and during pollen season as well as before and after allergen challenge. The seasonal material was used for mRNA quantification of Toll-like receptors 2, 3 and 4 with real-time polymerase chain reaction, whereas specimens achieved in conjunction with allergen challenge were used for immunohistochemical localization and quantification of corresponding proteins. RESULTS: mRNA and protein representing Toll-like receptors 2, 3 and 4 could be demonstrated in all specimens. An increase in protein expression for all three receptors could be seen following allergen challenge, whereas a significant increase of mRNA only could be obtained for Toll-like receptor 3 during pollen season. CONCLUSION: The up-regulation of Toll-like receptors 2, 3 and 4 in the nasal mucosa of patients with symptomatic allergic rhinitis supports the idea of a role for Toll-like receptors in allergic airway inflammation.}},
author = {{Fransson, Mattias and Adner, Mikael and Erjefält, Jonas and Jansson, Lennart and Uddman, Rolf and Cardell, Lars-Olaf}},
issn = {{1465-9921}},
language = {{eng}},
number = {{100}},
publisher = {{BioMed Central (BMC)}},
series = {{Respiratory Research}},
title = {{Up-regulation of Toll-like receptors 2, 3 and 4 in allergic rhinitis}},
url = {{http://dx.doi.org/10.1186/1465-9921-6-100}},
doi = {{10.1186/1465-9921-6-100}},
volume = {{6}},
year = {{2005}},
}