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Two-photon excitation in fluorescence polarization receptor-ligand binding assay

Tirri, M E; Huttunen, R J; Toivonen, J; Härkönen, Pirkko LU ; Soini, J T and Hanninen, P E (2005) In Journal of Biomolecular Screening 10(4). p.314-319
Abstract
Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
two-photon excitation, fluorescence polarization, high-throughput screening, estrogen receptor, drug discovery
in
Journal of Biomolecular Screening
volume
10
issue
4
pages
314 - 319
publisher
SAGE Publications Inc.
external identifiers
  • scopus:20444425639
ISSN
1087-0571
DOI
10.1177/1087057104273334
language
English
LU publication?
yes
id
696fc8f7-d1a6-44a3-86c6-9d05b967b731 (old id 1133685)
date added to LUP
2008-06-17 14:18:54
date last changed
2017-01-01 05:06:15
@article{696fc8f7-d1a6-44a3-86c6-9d05b967b731,
  abstract     = {Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.},
  author       = {Tirri, M E and Huttunen, R J and Toivonen, J and Härkönen, Pirkko and Soini, J T and Hanninen, P E},
  issn         = {1087-0571},
  keyword      = {two-photon excitation,fluorescence polarization,high-throughput screening,estrogen receptor,drug discovery},
  language     = {eng},
  number       = {4},
  pages        = {314--319},
  publisher    = {SAGE Publications Inc.},
  series       = {Journal of Biomolecular Screening},
  title        = {Two-photon excitation in fluorescence polarization receptor-ligand binding assay},
  url          = {http://dx.doi.org/10.1177/1087057104273334},
  volume       = {10},
  year         = {2005},
}