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HAMLET; a novel tool to identify apoptotic pathways in tumor cells.

Düringer, Caroline LU ; Hallgren, Oskar LU ; Gustafsson, Lotta LU ; Pettersson, Jenny LU ; Mossberg, Anki LU ; Manolov, Taras LU and Svanborg, Catharina LU (2005) In Application of apoptosis to cancer treatment. p.223-245
Abstract
Tumor cells often carry mutations in genes that control cell survival, and become resistant to signals that trigger cell death. Yet, some cell death pathways remain intact in tumor cells. If identified, these pathways might be exploited to selectively remove tumor cells. HAMLET (human α-lactalbumin made lethal to tumor cells) is a protein-lipid complex derived from human milk that activates cell death programs in tumor cells but not in healthy differentiated cells. We use HAMLET as a tool to identify apoptosis and apoptosis-like cell death mechanisms in tumor cells and to understand if these mechanisms differ between tumor and healthy cells. HAMLET interacts with the cell surface, translocates into the cytoplasm and accumulates in cell... (More)
Tumor cells often carry mutations in genes that control cell survival, and become resistant to signals that trigger cell death. Yet, some cell death pathways remain intact in tumor cells. If identified, these pathways might be exploited to selectively remove tumor cells. HAMLET (human α-lactalbumin made lethal to tumor cells) is a protein-lipid complex derived from human milk that activates cell death programs in tumor cells but not in healthy differentiated cells. We use HAMLET as a tool to identify apoptosis and apoptosis-like cell death mechanisms in tumor cells and to understand if these mechanisms differ between tumor and healthy cells. HAMLET interacts with the cell surface, translocates into the cytoplasm and accumulates in cell nuclei, where it disrupts the chromatin. Recent in vivo studies have shown that HAMLET maintains the tumoricidal activity in glioblastoma, papilloma and bladder cancer models, with no significant side effects. The results suggest that HAMLET should be explored as a new therapeutic agent with selectivity for the tumor and with little toxicity for adjacent healthy tissue. Such therapies are a much-needed complement to conventional treatments, to reduce the side effects and improve the selectivity. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
HAMLET, lactalbumin, cancer, apoptosis, apoptosis-like, programmed cell death, alpha- histone, p53, bcl-2, caspase, glioblastoma, HPV
in
Application of apoptosis to cancer treatment.
editor
Mels, Sluyser and
pages
223 - 245
publisher
Springer
external identifiers
  • scopus:84900583644
ISBN
978-1-4020-3303-2
DOI
10.1007/1-4020-3302-8_10
language
English
LU publication?
yes
id
e8a5adeb-3e7e-435a-a85d-59eace874ee6 (old id 1133837)
date added to LUP
2008-06-23 16:50:33
date last changed
2017-01-01 08:02:47
@inbook{e8a5adeb-3e7e-435a-a85d-59eace874ee6,
  abstract     = {Tumor cells often carry mutations in genes that control cell survival, and become resistant to signals that trigger cell death. Yet, some cell death pathways remain intact in tumor cells. If identified, these pathways might be exploited to selectively remove tumor cells. HAMLET (human α-lactalbumin made lethal to tumor cells) is a protein-lipid complex derived from human milk that activates cell death programs in tumor cells but not in healthy differentiated cells. We use HAMLET as a tool to identify apoptosis and apoptosis-like cell death mechanisms in tumor cells and to understand if these mechanisms differ between tumor and healthy cells. HAMLET interacts with the cell surface, translocates into the cytoplasm and accumulates in cell nuclei, where it disrupts the chromatin. Recent in vivo studies have shown that HAMLET maintains the tumoricidal activity in glioblastoma, papilloma and bladder cancer models, with no significant side effects. The results suggest that HAMLET should be explored as a new therapeutic agent with selectivity for the tumor and with little toxicity for adjacent healthy tissue. Such therapies are a much-needed complement to conventional treatments, to reduce the side effects and improve the selectivity.},
  author       = {Düringer, Caroline and Hallgren, Oskar and Gustafsson, Lotta and Pettersson, Jenny and Mossberg, Anki and Manolov, Taras and Svanborg, Catharina},
  editor       = {Mels, Sluyser},
  isbn         = {978-1-4020-3303-2},
  keyword      = {HAMLET,lactalbumin,cancer,apoptosis,apoptosis-like,programmed cell death,alpha- histone,p53,bcl-2,caspase,glioblastoma,HPV},
  language     = {eng},
  pages        = {223--245},
  publisher    = {Springer},
  series       = {Application of apoptosis to cancer treatment.},
  title        = {HAMLET; a novel tool to identify apoptotic pathways in tumor cells.},
  url          = {http://dx.doi.org/10.1007/1-4020-3302-8_10},
  year         = {2005},
}