Advanced

Differential effects of left and right neuropathy on opioid gene expression in lumbar spinal cord

Kononenko, Olga; Mityakina, Irina; Galatenko, Vladimir; Watanabe, Hiroyuki LU ; Bazov, Igor; Gerashchenko, Anna; Sarkisyan, Daniil; Iatsyshyna, Anna; Yakovleva, Tatiana and Tonevitsky, Alex, et al. (2018) In Brain Research 1695. p.78-83
Abstract

The endogenous opioid system (EOS) controls the processing of nociceptive stimuli and is a pharmacological target for opioids. Alterations in expression of the EOS genes under neuropathic pain condition may account for low efficacy of opioid drugs. We here examined whether EOS expression patterns are altered in the lumbar spinal cord of the rats with spinal nerve ligation (SNL) as a neuropathic pain model. Effects of the left- and right-side SNL on expression of EOS genes in the ipsi- and contralateral spinal domains were analysed. The SNL-induced changes were complex and different between the genes; between the dorsal and ventral spinal domains; and between the left and right sides of the spinal cord. Prodynorphin (Pdyn) expression was... (More)

The endogenous opioid system (EOS) controls the processing of nociceptive stimuli and is a pharmacological target for opioids. Alterations in expression of the EOS genes under neuropathic pain condition may account for low efficacy of opioid drugs. We here examined whether EOS expression patterns are altered in the lumbar spinal cord of the rats with spinal nerve ligation (SNL) as a neuropathic pain model. Effects of the left- and right-side SNL on expression of EOS genes in the ipsi- and contralateral spinal domains were analysed. The SNL-induced changes were complex and different between the genes; between the dorsal and ventral spinal domains; and between the left and right sides of the spinal cord. Prodynorphin (Pdyn) expression was upregulated in the ipsilateral dorsal domains by each the left and right-side SNL, while changes in expression of μ-opioid receptor (Oprm1) and proenkephalin (Penk) genes were dependent on the SNL side. Changes in expression of the Pdyn and κ-opioid receptor (Oprk1) genes were coordinated between the ipsi- and contralateral sides. Withdrawal response thresholds, indicators of mechanical allodynia correlated negatively with Pdyn expression in the right ventral domain after right side SNL. These findings suggest multiple roles of the EOS gene products in spinal sensitization and changes in motor reflexes, which may differ between the left and right sides.

(Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Gene expression, Lateralization, Lumbar spinal cord, Neuropathic pain, Opioid system, Rat model
in
Brain Research
volume
1695
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:85048514369
ISSN
0006-8993
DOI
10.1016/j.brainres.2018.05.043
language
English
LU publication?
yes
id
1134fa22-df50-4a55-b084-941e39434ea5
date added to LUP
2018-06-27 16:00:02
date last changed
2019-02-20 11:20:59
@article{1134fa22-df50-4a55-b084-941e39434ea5,
  abstract     = {<p>The endogenous opioid system (EOS) controls the processing of nociceptive stimuli and is a pharmacological target for opioids. Alterations in expression of the EOS genes under neuropathic pain condition may account for low efficacy of opioid drugs. We here examined whether EOS expression patterns are altered in the lumbar spinal cord of the rats with spinal nerve ligation (SNL) as a neuropathic pain model. Effects of the left- and right-side SNL on expression of EOS genes in the ipsi- and contralateral spinal domains were analysed. The SNL-induced changes were complex and different between the genes; between the dorsal and ventral spinal domains; and between the left and right sides of the spinal cord. Prodynorphin (Pdyn) expression was upregulated in the ipsilateral dorsal domains by each the left and right-side SNL, while changes in expression of μ-opioid receptor (Oprm1) and proenkephalin (Penk) genes were dependent on the SNL side. Changes in expression of the Pdyn and κ-opioid receptor (Oprk1) genes were coordinated between the ipsi- and contralateral sides. Withdrawal response thresholds, indicators of mechanical allodynia correlated negatively with Pdyn expression in the right ventral domain after right side SNL. These findings suggest multiple roles of the EOS gene products in spinal sensitization and changes in motor reflexes, which may differ between the left and right sides.</p>},
  author       = {Kononenko, Olga and Mityakina, Irina and Galatenko, Vladimir and Watanabe, Hiroyuki and Bazov, Igor and Gerashchenko, Anna and Sarkisyan, Daniil and Iatsyshyna, Anna and Yakovleva, Tatiana and Tonevitsky, Alex and Marklund, Niklas and Ossipov, Michael H. and Bakalkin, Georgy},
  issn         = {0006-8993},
  keyword      = {Gene expression,Lateralization,Lumbar spinal cord,Neuropathic pain,Opioid system,Rat model},
  language     = {eng},
  month        = {09},
  pages        = {78--83},
  publisher    = {Elsevier},
  series       = {Brain Research},
  title        = {Differential effects of left and right neuropathy on opioid gene expression in lumbar spinal cord},
  url          = {http://dx.doi.org/10.1016/j.brainres.2018.05.043},
  volume       = {1695},
  year         = {2018},
}