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Results of the first World Health Organization international collaborative study of detection of Human Papillomavirus DNA.

Quint, W G V ; Pagliusi, S ; Lelie, N ; de Villiers, E M ; Wheeler, C M ; Dillner, Joakim LU and Human Papillomavirus DNA international collaborative study group., World Health Organization Human Papillomavirus DNA international collaborative study group. (2006) In Journal of Clinical Microbiology 44(2). p.571-579
Abstract
Twenty-nine laboratories in 12 countries participated in a study to assess the performance of various human

papillomavirus (HPV) detection assays through the use of a recombinant HPV DNA standard reagent panel.

The panel was designed by a group of HPV experts, and samples were prepared and distributed by the World

Health Organization International Laboratory for Standards and Biologicals in The Netherlands. Each panel

consisted of 24 coded samples including a dilution series for HPV types 16 and 18, alone or in combination with

five other high-risk (HR) HPV types including HPV types 31, 33, 35, 45, and 52, the low-risk HPV type 6, and

a negative control. Qualitative assays were... (More)
Twenty-nine laboratories in 12 countries participated in a study to assess the performance of various human

papillomavirus (HPV) detection assays through the use of a recombinant HPV DNA standard reagent panel.

The panel was designed by a group of HPV experts, and samples were prepared and distributed by the World

Health Organization International Laboratory for Standards and Biologicals in The Netherlands. Each panel

consisted of 24 coded samples including a dilution series for HPV types 16 and 18, alone or in combination with

five other high-risk (HR) HPV types including HPV types 31, 33, 35, 45, and 52, the low-risk HPV type 6, and

a negative control. Qualitative assays were generally consistent across laboratories, and most invalid results

reflected a lack of HPV test sensitivity. The combined data sets had a proficiency for HPV 16 of 62.5% (15/24)

and for HPV 18 of 73.9% (17/23). HPV 31 was the least accurately detected by participating laboratories.

Approximately half of participating laboratories failed to detect high concentrations of HPV 31 and, to a lesser

extent, to detect HPV types 35, 52, and 6. The panel sample materials offer a source of renewable and

reproducible material that could be used in the future development of international standard reagents for

calibration of HPV DNA assays and kits. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Microbiology
volume
44
issue
2
pages
571 - 579
publisher
American Society for Microbiology
external identifiers
  • scopus:32344444715
ISSN
1098-660X
DOI
10.1128/JCM.44.2.571–579.2006
language
English
LU publication?
yes
id
14be53a5-4c61-43b1-bbae-836aecf1f67f (old id 1135939)
date added to LUP
2016-04-01 16:20:29
date last changed
2021-06-08 04:00:31
@article{14be53a5-4c61-43b1-bbae-836aecf1f67f,
  abstract     = {Twenty-nine laboratories in 12 countries participated in a study to assess the performance of various human<br/><br>
papillomavirus (HPV) detection assays through the use of a recombinant HPV DNA standard reagent panel.<br/><br>
The panel was designed by a group of HPV experts, and samples were prepared and distributed by the World<br/><br>
Health Organization International Laboratory for Standards and Biologicals in The Netherlands. Each panel<br/><br>
consisted of 24 coded samples including a dilution series for HPV types 16 and 18, alone or in combination with<br/><br>
five other high-risk (HR) HPV types including HPV types 31, 33, 35, 45, and 52, the low-risk HPV type 6, and<br/><br>
a negative control. Qualitative assays were generally consistent across laboratories, and most invalid results<br/><br>
reflected a lack of HPV test sensitivity. The combined data sets had a proficiency for HPV 16 of 62.5% (15/24)<br/><br>
and for HPV 18 of 73.9% (17/23). HPV 31 was the least accurately detected by participating laboratories.<br/><br>
Approximately half of participating laboratories failed to detect high concentrations of HPV 31 and, to a lesser<br/><br>
extent, to detect HPV types 35, 52, and 6. The panel sample materials offer a source of renewable and<br/><br>
reproducible material that could be used in the future development of international standard reagents for<br/><br>
calibration of HPV DNA assays and kits.},
  author       = {Quint, W G V and Pagliusi, S and Lelie, N and de Villiers, E M and Wheeler, C M and Dillner, Joakim and Human Papillomavirus DNA international collaborative study group., World Health Organization Human Papillomavirus DNA international collaborative study group.},
  issn         = {1098-660X},
  language     = {eng},
  number       = {2},
  pages        = {571--579},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Clinical Microbiology},
  title        = {Results of the first World Health Organization international collaborative study of detection of Human Papillomavirus DNA.},
  url          = {http://dx.doi.org/10.1128/JCM.44.2.571–579.2006},
  doi          = {10.1128/JCM.44.2.571–579.2006},
  volume       = {44},
  year         = {2006},
}