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Comparison of the proximal promoter regions of the PAX3 and PAX7 genes

Möller, Emely LU ; Isaksson, Margareth LU ; Mandahl, Nils LU ; Mertens, Fredrik LU and Panagopoulos, Ioannis LU (2007) In Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00 178(2). p.114-119
Abstract

Translocations t(2;13)(q35;q14) and t(1;13)(p36;q14), which fuse PAX3 and PAX7, respectively, to FOXO1A, characterize alveolar rhabdomyosarcoma. Previous studies have suggested that the expression of PAX7-FOXO1A is copy-number dependent, but that of PAX3-FOXO1A is not, which may be due to a weaker PAX7 than PAX3 promoter. The aim of the present study was to compare the transcriptional activities of the PAX3 and PAX7 proximal promoter regions, using the dual-luciferase reporter assay with three vector systems in eight cell lines. The PAX3 promoter was found to have higher transcriptional activity than that of PAX7 irrespective of the vector system or cell line used. These findings are consistent with the idea that an amplification event... (More)

Translocations t(2;13)(q35;q14) and t(1;13)(p36;q14), which fuse PAX3 and PAX7, respectively, to FOXO1A, characterize alveolar rhabdomyosarcoma. Previous studies have suggested that the expression of PAX7-FOXO1A is copy-number dependent, but that of PAX3-FOXO1A is not, which may be due to a weaker PAX7 than PAX3 promoter. The aim of the present study was to compare the transcriptional activities of the PAX3 and PAX7 proximal promoter regions, using the dual-luciferase reporter assay with three vector systems in eight cell lines. The PAX3 promoter was found to have higher transcriptional activity than that of PAX7 irrespective of the vector system or cell line used. These findings are consistent with the idea that an amplification event is required for the PAX7-FOXO1A chimeric transcript to reach a critical expression level.

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keywords
3T3 Cells, Animals, Cell Line, Child, Chromosome Aberrations, DNA Primers, Gene Amplification, Genes, Reporter, HeLa Cells, Humans, Mice, PAX7 Transcription Factor, Paired Box Transcription Factors, Plasmids, Promoter Regions, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Rhabdomyosarcoma, Transcription, Genetic, Transfection, Translocation, Genetic, Journal Article, Research Support, Non-U.S. Gov't
in
Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00
volume
178
issue
2
pages
6 pages
publisher
Elsevier
external identifiers
  • pmid:17954266
  • wos:000250688800004
  • scopus:35348911885
ISSN
0165-4608
DOI
10.1016/j.cancergencyto.2007.06.006
language
English
LU publication?
yes
id
cef36d86-db86-4cae-80b2-d101625b0297 (old id 1138767)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17954266&dopt=Abstract
date added to LUP
2008-08-19 16:06:56
date last changed
2017-01-03 09:32:39
@article{cef36d86-db86-4cae-80b2-d101625b0297,
  abstract     = {<p>Translocations t(2;13)(q35;q14) and t(1;13)(p36;q14), which fuse PAX3 and PAX7, respectively, to FOXO1A, characterize alveolar rhabdomyosarcoma. Previous studies have suggested that the expression of PAX7-FOXO1A is copy-number dependent, but that of PAX3-FOXO1A is not, which may be due to a weaker PAX7 than PAX3 promoter. The aim of the present study was to compare the transcriptional activities of the PAX3 and PAX7 proximal promoter regions, using the dual-luciferase reporter assay with three vector systems in eight cell lines. The PAX3 promoter was found to have higher transcriptional activity than that of PAX7 irrespective of the vector system or cell line used. These findings are consistent with the idea that an amplification event is required for the PAX7-FOXO1A chimeric transcript to reach a critical expression level.</p>},
  author       = {Möller, Emely and Isaksson, Margareth and Mandahl, Nils and Mertens, Fredrik and Panagopoulos, Ioannis},
  issn         = {0165-4608},
  keyword      = {3T3 Cells,Animals,Cell Line,Child,Chromosome Aberrations,DNA Primers,Gene Amplification,Genes, Reporter,HeLa Cells,Humans,Mice,PAX7 Transcription Factor,Paired Box Transcription Factors,Plasmids,Promoter Regions, Genetic,Reverse Transcriptase Polymerase Chain Reaction,Rhabdomyosarcoma,Transcription, Genetic,Transfection,Translocation, Genetic,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {10},
  number       = {2},
  pages        = {114--119},
  publisher    = {Elsevier},
  series       = {Cancer Genetics and Cytogenetics1979-01-01+01:002011-01-01+01:00},
  title        = {Comparison of the proximal promoter regions of the PAX3 and PAX7 genes},
  url          = {http://dx.doi.org/10.1016/j.cancergencyto.2007.06.006},
  volume       = {178},
  year         = {2007},
}