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The pentraxin serum amyloid P component is found in the male genital tract and attached to spermatozoa

Malm, Johan LU ; Sonesson, Annika LU ; Hellman, Jukka; Bjartell, Anders LU ; Frohm, Birgitta LU and Hillarp, Andreas LU (2008) In International Journal of Andrology 31. p.508-517
Abstract
Serum amyloid P component (SAP) belongs to the pentraxin family of proteins, members of which are characterized by radial pentameric structure and calcium-dependent ligand binding. SAP is present in all types of amyloidosis and has been shown to bind to several ligands, but the physiological function of this protein has not been fully elucidated. The present study identified and characterized SAP in human semen and immunolocalized it to the male reproductive tract. SAP was also detected in seminal plasma by immunoblotting and purification by affinity chromatography followed by mass spectrometry. According to electroimmunoassay, the concentration of SAP in semen is approximately 2 mg/L, and flow cytometry revealed SAP attached to the... (More)
Serum amyloid P component (SAP) belongs to the pentraxin family of proteins, members of which are characterized by radial pentameric structure and calcium-dependent ligand binding. SAP is present in all types of amyloidosis and has been shown to bind to several ligands, but the physiological function of this protein has not been fully elucidated. The present study identified and characterized SAP in human semen and immunolocalized it to the male reproductive tract. SAP was also detected in seminal plasma by immunoblotting and purification by affinity chromatography followed by mass spectrometry. According to electroimmunoassay, the concentration of SAP in semen is approximately 2 mg/L, and flow cytometry revealed SAP attached to the surface of spermatozoa. Moreover, immunohistochemistry showed positive staining of spermatozoa, subsets of epithelial cells, and the stroma of accessory male genital glands and testis. Presence of mRNA supports local production of SAP, as shown with reverse transcription polymerase chain reaction. We identified SAP in a new setting - the human male reproductive system. SAP was detected on ejaculated spermatozoa, in seminal plasma and in tissue sections from the male reproductive tract. Further functional studies are needed to explain the role of SAP in human reproduction. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
reproduction, semen, serum amyloid P component, spermatozoa, pentraxins
in
International Journal of Andrology
volume
31
pages
508 - 517
publisher
Wiley-Blackwell
external identifiers
  • pmid:17822421
  • wos:000259025400009
  • scopus:51349112136
ISSN
0105-6263
DOI
10.1111/j.1365-2605.2007.00800.x
language
English
LU publication?
yes
id
bf671c0b-f1d1-4f06-bc04-11e8eec2a95c (old id 1139985)
date added to LUP
2008-08-21 11:25:10
date last changed
2017-08-27 04:35:28
@article{bf671c0b-f1d1-4f06-bc04-11e8eec2a95c,
  abstract     = {Serum amyloid P component (SAP) belongs to the pentraxin family of proteins, members of which are characterized by radial pentameric structure and calcium-dependent ligand binding. SAP is present in all types of amyloidosis and has been shown to bind to several ligands, but the physiological function of this protein has not been fully elucidated. The present study identified and characterized SAP in human semen and immunolocalized it to the male reproductive tract. SAP was also detected in seminal plasma by immunoblotting and purification by affinity chromatography followed by mass spectrometry. According to electroimmunoassay, the concentration of SAP in semen is approximately 2 mg/L, and flow cytometry revealed SAP attached to the surface of spermatozoa. Moreover, immunohistochemistry showed positive staining of spermatozoa, subsets of epithelial cells, and the stroma of accessory male genital glands and testis. Presence of mRNA supports local production of SAP, as shown with reverse transcription polymerase chain reaction. We identified SAP in a new setting - the human male reproductive system. SAP was detected on ejaculated spermatozoa, in seminal plasma and in tissue sections from the male reproductive tract. Further functional studies are needed to explain the role of SAP in human reproduction.},
  author       = {Malm, Johan and Sonesson, Annika and Hellman, Jukka and Bjartell, Anders and Frohm, Birgitta and Hillarp, Andreas},
  issn         = {0105-6263},
  keyword      = {reproduction,semen,serum amyloid P component,spermatozoa,pentraxins},
  language     = {eng},
  pages        = {508--517},
  publisher    = {Wiley-Blackwell},
  series       = {International Journal of Andrology},
  title        = {The pentraxin serum amyloid P component is found in the male genital tract and attached to spermatozoa},
  url          = {http://dx.doi.org/10.1111/j.1365-2605.2007.00800.x},
  volume       = {31},
  year         = {2008},
}