Calcium addition to EDTA plasma eliminates falsely positive results in the RSR GADAb ELISA
Nilson, Emma LU ; Ekholm, Birgitte LU ; Rees Smith, B ; Törn, Carina LU and Hillman, Magnus LU
(2008)
In Clinica Chimica Acta
388(1-2).
p.130-134
- Abstract
- BACKGROUND: The RSR ELISAs for GADAb and IA-2Ab are now widely used but due to a considerable number of falsely positive GADAb results obtained with EDTA plasma, it is recommended that only serum samples are run in these two assays. However, as EDTA plasma is often the preferred sample type in Europe we have assessed the possibility that adding Ca(2+) to plasma would reduce the number of falsely positive results. METHODS: Antibody positive as well as antibody negative diabetic subjects were included in the study (n=80). Serum and EDTA plasma were collected from the same subjects at the same occasion. Samples were divided into three groups; sera, EDTA plasma and EDTA plasma+Ca(2+). ELISA kits were supplied by RSR((R)) Ltd and performed... (More)
- BACKGROUND: The RSR ELISAs for GADAb and IA-2Ab are now widely used but due to a considerable number of falsely positive GADAb results obtained with EDTA plasma, it is recommended that only serum samples are run in these two assays. However, as EDTA plasma is often the preferred sample type in Europe we have assessed the possibility that adding Ca(2+) to plasma would reduce the number of falsely positive results. METHODS: Antibody positive as well as antibody negative diabetic subjects were included in the study (n=80). Serum and EDTA plasma were collected from the same subjects at the same occasion. Samples were divided into three groups; sera, EDTA plasma and EDTA plasma+Ca(2+). ELISA kits were supplied by RSR((R)) Ltd and performed according to the manufacturers instructions. RESULTS: GADAb analyses with plasma showed a markedly increased prevalence of GADAb positive subjects (81%) compared to sera (36%), indicating falsely positive results with plasma. Addition of Ca(2+) reduced the number of GADAb positive results to the same number obtained with serum. IA-2Ab positivity was not different between sera and plasma. CONCLUSION: EDTA plasma can be used in the RSR GADAb and IA-2Ab ELISAs if Ca(2+) is added prior to assay. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1142381
- author
- Nilson, Emma
LU
; Ekholm, Birgitte
LU
; Rees Smith, B
; Törn, Carina
LU
and Hillman, Magnus
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Clinica Chimica Acta
- volume
- 388
- issue
- 1-2
- pages
- 130 - 134
- publisher
- Elsevier
- external identifiers
-
- pmid:18023278
- wos:000252770800021
- scopus:37249029951
- pmid:18023278
- ISSN
- 0009-8981
- DOI
- 10.1016/j.cca.2007.10.021
- language
- English
- LU publication?
- yes
- id
- d6c6a0db-6461-4abf-aa8b-829a7bd6cd47 (old id 1142381)
- date added to LUP
- 2016-04-01 12:15:07
- date last changed
- 2024-12-05 09:13:48
@article{d6c6a0db-6461-4abf-aa8b-829a7bd6cd47,
abstract = {{BACKGROUND: The RSR ELISAs for GADAb and IA-2Ab are now widely used but due to a considerable number of falsely positive GADAb results obtained with EDTA plasma, it is recommended that only serum samples are run in these two assays. However, as EDTA plasma is often the preferred sample type in Europe we have assessed the possibility that adding Ca(2+) to plasma would reduce the number of falsely positive results. METHODS: Antibody positive as well as antibody negative diabetic subjects were included in the study (n=80). Serum and EDTA plasma were collected from the same subjects at the same occasion. Samples were divided into three groups; sera, EDTA plasma and EDTA plasma+Ca(2+). ELISA kits were supplied by RSR((R)) Ltd and performed according to the manufacturers instructions. RESULTS: GADAb analyses with plasma showed a markedly increased prevalence of GADAb positive subjects (81%) compared to sera (36%), indicating falsely positive results with plasma. Addition of Ca(2+) reduced the number of GADAb positive results to the same number obtained with serum. IA-2Ab positivity was not different between sera and plasma. CONCLUSION: EDTA plasma can be used in the RSR GADAb and IA-2Ab ELISAs if Ca(2+) is added prior to assay.}},
author = {{Nilson, Emma and Ekholm, Birgitte and Rees Smith, B and Törn, Carina and Hillman, Magnus}},
issn = {{0009-8981}},
language = {{eng}},
number = {{1-2}},
pages = {{130--134}},
publisher = {{Elsevier}},
series = {{Clinica Chimica Acta}},
title = {{Calcium addition to EDTA plasma eliminates falsely positive results in the RSR GADAb ELISA}},
url = {{http://dx.doi.org/10.1016/j.cca.2007.10.021}},
doi = {{10.1016/j.cca.2007.10.021}},
volume = {{388}},
year = {{2008}},
}