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Rapid DNA library construction for functional genomic and metagenomic screening

Schmitz, Jonathan E; Daniel, Anu; Collin, Mattias LU ; Schuch, Raymond and Fischetti, Vincent A (2008) In Applied and Environmental Microbiology 74(5). p.1649-1652
Abstract
A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
74
issue
5
pages
1649 - 1652
publisher
American Society for Microbiology
external identifiers
  • pmid:18083885
  • scopus:40549144318
ISSN
0099-2240
DOI
10.1128/AEM.01864-07
language
English
LU publication?
no
id
107b3865-d187-43ea-9878-a055552ca567 (old id 1144726)
date added to LUP
2008-08-14 16:38:04
date last changed
2017-05-28 03:32:30
@article{107b3865-d187-43ea-9878-a055552ca567,
  abstract     = {A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.},
  author       = {Schmitz, Jonathan E and Daniel, Anu and Collin, Mattias and Schuch, Raymond and Fischetti, Vincent A},
  issn         = {0099-2240},
  language     = {eng},
  number       = {5},
  pages        = {1649--1652},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Rapid DNA library construction for functional genomic and metagenomic screening},
  url          = {http://dx.doi.org/10.1128/AEM.01864-07},
  volume       = {74},
  year         = {2008},
}